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Registro Completo |
Biblioteca(s): |
Embrapa Agricultura Digital. |
Data corrente: |
29/09/2014 |
Data da última atualização: |
29/09/2014 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
TORRES, T. Z. |
Afiliação: |
TERCIA ZAVAGLIA TORRES, CNPTIA. |
Título: |
Recrutamento e seleção de pessoas. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
In: TORRES, T. Z.; MAZIERO, M. das D. S.; VEDOVELLO, J. G. Gestão de pessoas: tecnologias e processos. Campo Limpo Paulista: Faccamp, 2014. |
Páginas: |
p. 49-70 |
Idioma: |
Português |
Conteúdo: |
Os processos no cenário empresarial conteporâneo. Mercado de trabalho e necessidades empresaraiis. Recrutamento: conceitos e técnicas. Processo de seleção de pessoal. Processos de recrutamento e seleção por competências. |
Palavras-Chave: |
Desempenho organizacional; Seleção de pessoal; Talento humano. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00763naa a2200169 a 4500 001 1996009 005 2014-09-29 008 2014 bl uuuu u00u1 u #d 100 1 $aTORRES, T. Z. 245 $aRecrutamento e seleção de pessoas. 260 $c2014 300 $ap. 49-70 520 $aOs processos no cenário empresarial conteporâneo. Mercado de trabalho e necessidades empresaraiis. Recrutamento: conceitos e técnicas. Processo de seleção de pessoal. Processos de recrutamento e seleção por competências. 653 $aDesempenho organizacional 653 $aSeleção de pessoal 653 $aTalento humano 773 $tIn: TORRES, T. Z.; MAZIERO, M. das D. S.; VEDOVELLO, J. G. Gestão de pessoas: tecnologias e processos. Campo Limpo Paulista: Faccamp, 2014.
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Embrapa Agricultura Digital (CNPTIA) |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
29/12/2014 |
Data da última atualização: |
30/12/2014 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
POLIZEL-PODANOSQUI, A. M.; CARVALHO, M. C. C. G. de; ROCHA, C. S.; DARBEN, L. M.; LOPES-CAITAR, V. S.; AOYAOGI, L. N.; MARCELINO-GUIMARÃES, F. C. |
Afiliação: |
CNPSO - estagiário; UENP; CNPSO - estagiário; UENP; CNPSO - estagiário; CNPSO - estagiário; FRANCISMAR CORREA MARCELINO-GUIMARÃES, CNPSO. |
Título: |
Temporal gene expression analysis of effector candidates of Phakopsora pachyrhizi across the infection cycle. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
In: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 16., 2014, Rhodes. [Abstracts...]. Atenas: International Society of Molecular Plant-Microbe Interactions, 2014. |
Idioma: |
Inglês |
Conteúdo: |
Phakopsora pachyrhizi is the causer of the Asian soybean rust, the most aggressive foliar disease that attacks the soybean plants in cultivated areas. To design effective breeding strategies for durable disease resistance it is important to understand the molecular basis of this plant-pathogen interaction. In particular, the characterization of the secreted effector proteins can help the breeding programs. Our recent analysis of the P. pachyrhizi secretome has revealed the presence of potential effectors genes. In this work, the temporal expression of 58 effector candidate genes were characterized from spores, germinated spores and infected soybean leaves at 0, 6, 12, 24, 36, 48, 72, 96, 192 and 240 hours after inoculation (hai) using RT-qPCR. Expression analysis showed differential temporal gene expression of these candidate effectors. Clustering analysis revealed three main clusters with coordinated expression of candidates across the infection cycle. The first has candidates expressed in specific steps of infection and almost sequences have the common motifs Y/F/WxC frequently found in haustoria-producing rust fungi effectors. The second cluster has candidates expressed during initial steps of infection that matches germ tube and appressorium formation (0 – 24 hai) and almost sequences have ?3 cysteine residues, other common features of the effectors. Third cluster has candidates expressed only in contact with the host, suggesting that these candidates are host-induced. The sequences in this cluster have the CFEM domain associated with pathogenicity. Functional analyses are in progress with more promising candidates effectors to validate the potential effectors from soybean rust. MenosPhakopsora pachyrhizi is the causer of the Asian soybean rust, the most aggressive foliar disease that attacks the soybean plants in cultivated areas. To design effective breeding strategies for durable disease resistance it is important to understand the molecular basis of this plant-pathogen interaction. In particular, the characterization of the secreted effector proteins can help the breeding programs. Our recent analysis of the P. pachyrhizi secretome has revealed the presence of potential effectors genes. In this work, the temporal expression of 58 effector candidate genes were characterized from spores, germinated spores and infected soybean leaves at 0, 6, 12, 24, 36, 48, 72, 96, 192 and 240 hours after inoculation (hai) using RT-qPCR. Expression analysis showed differential temporal gene expression of these candidate effectors. Clustering analysis revealed three main clusters with coordinated expression of candidates across the infection cycle. The first has candidates expressed in specific steps of infection and almost sequences have the common motifs Y/F/WxC frequently found in haustoria-producing rust fungi effectors. The second cluster has candidates expressed during initial steps of infection that matches germ tube and appressorium formation (0 – 24 hai) and almost sequences have ?3 cysteine residues, other common features of the effectors. Third cluster has candidates expressed only in contact with the host, suggesting that these candidates are host-induced. T... Mostrar Tudo |
Palavras-Chave: |
Ferrugem asiática da soja. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/114606/1/Temporal-gene-expression-analysis-of-effector-candidates-of-Phakopsora-pachyrhizi-across-the-infection-cycle.pdf
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Marc: |
LEADER 02492nam a2200193 a 4500 001 2003884 005 2014-12-30 008 2014 bl uuuu u00u1 u #d 100 1 $aPOLIZEL-PODANOSQUI, A. M. 245 $aTemporal gene expression analysis of effector candidates of Phakopsora pachyrhizi across the infection cycle.$h[electronic resource] 260 $aIn: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 16., 2014, Rhodes. [Abstracts...]. Atenas: International Society of Molecular Plant-Microbe Interactions$c2014 520 $aPhakopsora pachyrhizi is the causer of the Asian soybean rust, the most aggressive foliar disease that attacks the soybean plants in cultivated areas. To design effective breeding strategies for durable disease resistance it is important to understand the molecular basis of this plant-pathogen interaction. In particular, the characterization of the secreted effector proteins can help the breeding programs. Our recent analysis of the P. pachyrhizi secretome has revealed the presence of potential effectors genes. In this work, the temporal expression of 58 effector candidate genes were characterized from spores, germinated spores and infected soybean leaves at 0, 6, 12, 24, 36, 48, 72, 96, 192 and 240 hours after inoculation (hai) using RT-qPCR. Expression analysis showed differential temporal gene expression of these candidate effectors. Clustering analysis revealed three main clusters with coordinated expression of candidates across the infection cycle. The first has candidates expressed in specific steps of infection and almost sequences have the common motifs Y/F/WxC frequently found in haustoria-producing rust fungi effectors. The second cluster has candidates expressed during initial steps of infection that matches germ tube and appressorium formation (0 – 24 hai) and almost sequences have ?3 cysteine residues, other common features of the effectors. Third cluster has candidates expressed only in contact with the host, suggesting that these candidates are host-induced. The sequences in this cluster have the CFEM domain associated with pathogenicity. Functional analyses are in progress with more promising candidates effectors to validate the potential effectors from soybean rust. 653 $aFerrugem asiática da soja 700 1 $aCARVALHO, M. C. C. G. de 700 1 $aROCHA, C. S. 700 1 $aDARBEN, L. M. 700 1 $aLOPES-CAITAR, V. S. 700 1 $aAOYAOGI, L. N. 700 1 $aMARCELINO-GUIMARÃES, F. C.
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