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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Norte / UEP-Parnaíba. |
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Data corrente: |
25/03/1996 |
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Data da última atualização: |
25/03/1996 |
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Autoria: |
ANGEL, F.; ARIAS, D. I.; TOHME, J.; IGLESIAS, C.; ROCA, W. M. |
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Título: |
Toward the construction of a molecular map of cassava (Manihot esculenta Crantz): comparison of restriction enzymes and probe sources in detecting RFLPs. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Journal of Biotechnology, v. 31, p. 103-113, 1993. |
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Idioma: |
Inglês |
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Conteúdo: |
The construction of a detailed genetic map of cassava (Manihot esculenta Crantz), classified as a tetraploid species, depends on the ability of cloned sequences to detect polymorphisms. As a firts step in developing this map, 200 cloned nuclear sequences generated with different restriction enzymes were hybridized to total digested DNA from eleven cultivated lines and one wild Manihot species, M. aesculifolia. Polymosphism was detected less frequently with both BamHI and EcoRI genomic probes than with PstI, HindIII and XbaI genomic probes. DNA digested with HpaII, DraI and TaqI displayd less polymosphism, whereas DNA digested with EcoRI and EcoRV displayed more polymosphism like that found in lettuce, rice and tomato (Landry et al., 1987; McCouch et al., 1988; Miller and Tanksley, 1990). Four-cutter restriction enzymes displayed less frequency of polymosphism when compared with six-cutter restriction enzymes. Polymosphism displayed by DraI was extremely low, indicating that regions rich in adenine and thymine may not be hot spots for mutation in cassava. Polymorphism detected between cultivated genetypes and M. aesculifolia was dramatically higher than that among cultivated genotypes. |
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Palavras-Chave: |
Enzima de restrição; Probe source; Restriction enzyme; RFLP; Sonda. |
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Thesagro: |
Mandioca. |
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Thesaurus Nal: |
cassava. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01936naa a2200253 a 4500
001 1076400
005 1996-03-25
008 1993 bl --- 0-- u #d
100 1 $aANGEL, F.
245 $aToward the construction of a molecular map of cassava (Manihot esculenta Crantz)$bcomparison of restriction enzymes and probe sources in detecting RFLPs.
260 $c1993
520 $aThe construction of a detailed genetic map of cassava (Manihot esculenta Crantz), classified as a tetraploid species, depends on the ability of cloned sequences to detect polymorphisms. As a firts step in developing this map, 200 cloned nuclear sequences generated with different restriction enzymes were hybridized to total digested DNA from eleven cultivated lines and one wild Manihot species, M. aesculifolia. Polymosphism was detected less frequently with both BamHI and EcoRI genomic probes than with PstI, HindIII and XbaI genomic probes. DNA digested with HpaII, DraI and TaqI displayd less polymosphism, whereas DNA digested with EcoRI and EcoRV displayed more polymosphism like that found in lettuce, rice and tomato (Landry et al., 1987; McCouch et al., 1988; Miller and Tanksley, 1990). Four-cutter restriction enzymes displayed less frequency of polymosphism when compared with six-cutter restriction enzymes. Polymosphism displayed by DraI was extremely low, indicating that regions rich in adenine and thymine may not be hot spots for mutation in cassava. Polymorphism detected between cultivated genetypes and M. aesculifolia was dramatically higher than that among cultivated genotypes.
650 $acassava
650 $aMandioca
653 $aEnzima de restrição
653 $aProbe source
653 $aRestriction enzyme
653 $aRFLP
653 $aSonda
700 1 $aARIAS, D. I.
700 1 $aTOHME, J.
700 1 $aIGLESIAS, C.
700 1 $aROCA, W. M.
773 $tJournal of Biotechnology$gv. 31, p. 103-113, 1993.
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Registro original: |
Embrapa Meio Norte / UEP-Parnaíba (CPAMN-UEPP) |
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| Registros recuperados : 7 | |
| 1. |  | PACHÓN, H.; BEEBE, S.; MARTÍNEZ, CÉSAR; GRENIER, C.; ATLIN, G.; SAN VICENTE, F.; GRUNEBER, W.; TOHME, J.; NUTTI, M. Biofortificación: programa global de nutrición en américa latina. In: REUNIÓN ANUAL , 59., 2014, Nicaragua. Programa cooperativo centroamericano para el mejoramiento de cultivos y animales. Programa. Nicaragua: INTA, 2014. p. 407. Ref. A956.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
|    |
| 2. | | MONCADA, P.; MARTINEZ, C. P.; BORRERO, J.; CHATEL, M.; GAUCH JR. H.; GUIMARÃES, E. P.; TOHME, J.; McCOUCH, S. R. Quantitative trait loci for yield and yield components in an Oryza sativa x Oryza rufipogon BC2F2 population evaluated in an upland environment. Theoretical and Applied Genetics, v. 102, n. 1, p. 41-52, 2001.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 3. | | DOMÍNGUEZ, M.; ORTIZ, D.; ARAGON, I. J.; CARVALHO, J. L. V. de; CEBALLOS, H.; BEEBE, S.; GRENIER, C.; TOHME, J.; NUTTI, M. Evaluación de micronutrientes en cultivos biofortificados. In: REUNIÓN ANUAL PROGRAMA COOPERATIVO CENTROAMERICANO PARA EL MEJORAMIENTO DE CULTIVOS Y ANIMALES - PCCMCA, 59., 2014, Managua, Nicaragua. Memoria de resmenes... Nicaragua, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 4. | | NUTTI, M. R.; BEEBE, S.; Grenier C.; SAN VICENTE, F.; GRUNEBERG, W.; CEBALLOS, H.; CARVALHO, J. L. V. de; TOHME, J.; GONZÁLEZ, C.; TALSMA, E. El progreso de acciones de biofortificacíon em America Latina y el Caribe. In: REUNIÓN ANUAL PROGRAMA COOPERATIVO CENTROAMERICANO PARA EL MEJORAMIENTO DE CULTIVOS Y ANIMALES, 62., 2017, El Salvador. Trabajos. El Salvador: [s.n.], 2017. 1 p. PCCMCA.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 5. | | NUTTI, M.; BEEBE, S.; GRENIER, C.; VICENTE, F. SAN; GRUNEBERG, W.; CEBALLOS, H.; CARVALHO, J. V. de; TOHME, J.; GONZÁLEZ, C.; TALSMA, E. Mejorando agricultura, mejorando nutrición. In: REUNIÓN ANUAL PROGRAMA COOPERATIVO CENTROAMERICANO PARA EL MEJORAMIENTO DE CULTIVOS Y ANIMALES, 61., 2016, Costa Rica. Trabajos. Costa Rica: Instituto de Ciencia y Tecnología Agrícola, 2016. 1 p. PCCMCA. LEG 47.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
|  |
| 6. | | PACHÓN, H.; BEEBE, S.; MARTÍNEZ, C.; GRENIER, C.; ATLIN, G.; SAN VICENTE, F.; GRUNEBERG, W.; CARVALHO, J. L. V. de; TOHME, J.; NUTTI, M. R.; GONZÁLEZ, C. Mejorando agricultura, mejorando nutrición. In: REUNIÓN ANUAL DE LA SOCIEDAD DEL PROGRAMA COOPERATIVO CENTRO AMERICANO PARA EL MEJORAMIENTO DE CULTIVOS Y ANIMALES, 60., 2015, Guatemala. Memoria de resúmenes...Ciudad de Guatemala, Guatemala, 2015. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 7. | | RAO, I.; PETERS, M.; CASTRO, A.; SCHULTZE-KRAFT, R.; WHITE, D.; FISHER, M.; MILE, J.; LASCANO, C.; BLÜMMEL, M.; BUNGENSTAB, D. J.; TAPASCO, J.; HYMAN, G.; BOLLIGER, A.; PAUL, B.; VAN DER HOEK, R.; MAASS, B.; TIEMANN, T.; CUCHILLO, M.; DOUXCHAMPS, S.; VILLANUEVA, C; RINCÓN, A.; AYARZA, M; ROSENSTOCK, T.; SUBBARAO, G.; ARANGO, J.; CARDOSO, J.; WORTHINGTON, M.; CHIRINDA, N.; NOTENBAERT, A.; JENET, A.; SCHMIDT, A.; VIVAS, N.; LEFROY, R.; FAHRNEY, K.; GUIMARÃES, E.; TOHME, J.; COOK, S.; HERRERO, M.; CHACÓN, M.; SEARCHINGER, T.; RUDEL, T. LivestockPlus - The sustainable intensification of forage-based agricultural systems to improve livelihoods and ecosystem services in the tropics. Tropical Grasslands - Forrajes Tropicales, v. 3, p. 59-82, 2015| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| Registros recuperados : 7 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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