Data/hora: 09/06/2024 11:43:20

Biblioteca(s): | Embrapa Meio Norte / UEP-Parnaíba.
Data corrente: | 25/03/1996
Data da última atualização: | 25/03/1996
Autoria: | ANGEL, F.; ARIAS, D. I.; TOHME, J.; IGLESIAS, C.; ROCA, W. M.
Título: | Toward the construction of a molecular map of cassava (Manihot esculenta Crantz): comparison of restriction enzymes and probe sources in detecting RFLPs.
Ano de publicação: | 1993
Fonte/Imprenta: | Journal of Biotechnology, v. 31, p. 103-113, 1993.
Idioma: | Inglês
Conteúdo: | The construction of a detailed genetic map of cassava (Manihot esculenta Crantz), classified as a tetraploid species, depends on the ability of cloned sequences to detect polymorphisms. As a firts step in developing this map, 200  cloned nuclear sequences generated with different restriction enzymes were hybridized to total digested DNA from eleven cultivated lines and one wild Manihot species, M. aesculifolia. Polymosphism was detected less frequently with both BamHI and EcoRI genomic probes than with PstI, HindIII and XbaI genomic probes. DNA digested with HpaII, DraI and TaqI displayd less polymosphism, whereas DNA digested with EcoRI and EcoRV displayed more polymosphism  like that found in lettuce, rice and tomato (Landry et al., 1987; McCouch et al., 1988; Miller and Tanksley, 1990). Four-cutter restriction enzymes displayed less frequency of polymosphism when compared with six-cutter restriction enzymes. Polymosphism displayed by DraI was extremely low, indicating that regions rich in adenine and thymine may not be hot spots for mutation in cassava. Polymorphism  detected between cultivated genetypes and M. aesculifolia  was dramatically higher than that among cultivated genotypes.
Palavras-Chave: | Enzima de restrição; Probe source; Restriction enzyme; RFLP; Sonda.
Thesagro: | Mandioca.
Thesaurus Nal: | cassava.
Categoria do assunto: | --
Registro original: | Embrapa Meio Norte / UEP-Parnaíba (CPAMN-UEPP)
Exemplares: | CPAMN-UEPP - (5416-1) - ADP - SP - -- - SP 079/1996 -  - 1996.00079 - DIS