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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
17/10/2012 |
Data da última atualização: |
16/09/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SCHERER, R.; RYBKA, A. C. P.; BALLUS, C. A.; MEINHART, A. D.; TEIXEIRA FILHO, J.; GODOY, H. T. |
Afiliação: |
RODRIGO SCHERER; ANA CECILIA POLONI RYBKA, CPATSA; CRISTIANO AUGUSTO BALLUS; ADRIANA DILLENBURG MEINHART; JOSÉ TEIXEIRA FILHO; HELENA TEIXEIRA GODOY. |
Título: |
Validation of a HPLC method for simultaneous determination of main organic acids in fruits and juices. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Food Chemistry, v. 135, p. 150-154, 2012. |
Idioma: |
Inglês |
Conteúdo: |
A liquid chromatographic method for fast and simultaneous determination of tartaric, malic, ascorbic and citric acids was validated for further application to fruits and juices. Moreover, the organic acids content of commercial samples of fruits and juices were evaluated, as well as the ascorbic acid stability during the storage. Determination of organic acids was carried out using a liquid chromatograph coupled to a diode array detector, with reversed phase (C18 column) and isocratic elution with 0.01 mol L1 KH2PO4 (pH = 2.60) mobile phase. The validation parameters showed efficiency, adequate linearity, relative standard deviation values between 0.4% and 2.3% (n = 10) for repeatability and from 1.2% to 5.0% (n = 18) for reproducibility, limits of detection (LD) were between 0.03 and 3.31 lg mL1 and quantification (LQ) were between 0.10 and 11.03 lg mL1, recovery rates were between 82% and 110%, for two levels. In addition, the method is fast (10 min) and generates low and non-toxic residues. The values found for vitamin C were about 10 times above the values declared at the package. Ready to drink juices have a composition similar to the fruit, concerning to organic acids, except for the powder juice, in which only ascorbic and citric acids were found, for all tastes. After opening the package, a decrease of 14.0% and 27.0% in ascorbic acid content was observed for orange powder and ready to drink juices, respectively. |
Palavras-Chave: |
Ácidos orgânicos; Cromatografia líquida; Estabilidade de ácido ascórbico; HPLC; Sucos de frutas; Validação do método. |
Thesagro: |
Fruta; Pós-Colheita; Processamento; Suco. |
Thesaurus Nal: |
Fruits; Juices. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/68195/1/Artigo-Ana-Cecilia-2012-1.pdf
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Marc: |
LEADER 02355naa a2200325 a 4500 001 1936986 005 2022-09-16 008 2012 bl uuuu u00u1 u #d 100 1 $aSCHERER, R. 245 $aValidation of a HPLC method for simultaneous determination of main organic acids in fruits and juices.$h[electronic resource] 260 $c2012 520 $aA liquid chromatographic method for fast and simultaneous determination of tartaric, malic, ascorbic and citric acids was validated for further application to fruits and juices. Moreover, the organic acids content of commercial samples of fruits and juices were evaluated, as well as the ascorbic acid stability during the storage. Determination of organic acids was carried out using a liquid chromatograph coupled to a diode array detector, with reversed phase (C18 column) and isocratic elution with 0.01 mol L1 KH2PO4 (pH = 2.60) mobile phase. The validation parameters showed efficiency, adequate linearity, relative standard deviation values between 0.4% and 2.3% (n = 10) for repeatability and from 1.2% to 5.0% (n = 18) for reproducibility, limits of detection (LD) were between 0.03 and 3.31 lg mL1 and quantification (LQ) were between 0.10 and 11.03 lg mL1, recovery rates were between 82% and 110%, for two levels. In addition, the method is fast (10 min) and generates low and non-toxic residues. The values found for vitamin C were about 10 times above the values declared at the package. Ready to drink juices have a composition similar to the fruit, concerning to organic acids, except for the powder juice, in which only ascorbic and citric acids were found, for all tastes. After opening the package, a decrease of 14.0% and 27.0% in ascorbic acid content was observed for orange powder and ready to drink juices, respectively. 650 $aFruits 650 $aJuices 650 $aFruta 650 $aPós-Colheita 650 $aProcessamento 650 $aSuco 653 $aÁcidos orgânicos 653 $aCromatografia líquida 653 $aEstabilidade de ácido ascórbico 653 $aHPLC 653 $aSucos de frutas 653 $aValidação do método 700 1 $aRYBKA, A. C. P. 700 1 $aBALLUS, C. A. 700 1 $aMEINHART, A. D. 700 1 $aTEIXEIRA FILHO, J. 700 1 $aGODOY, H. T. 773 $tFood Chemistry$gv. 135, p. 150-154, 2012.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Rondônia. |
Data corrente: |
29/06/2023 |
Data da última atualização: |
18/07/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
SILVA, S. A.; MONDADORI, R. G.; NOLETO, G. S.; BARBOSA, I. P.; GONÇALVES, R. L.; GASPERIN, B. G.; ROVANI, M. T.; PAZ, E. F.; GOMES, L. S.; PFEIFER, L. F. M. |
Afiliação: |
SAMIRA A. SILVA, UNIVERSIDADE FEDERAL DE PELOTAS; RAFAEL G. MONDADORI, UNIVERSIDADE FEDERAL DE PELOTAS; GABRIELLY S. NOLETO, UNIVERSIDADE FEDERAL DE RONDÔNIA; INGRID P. BARBOSA, UNIVERSIDADE FEDERAL DO ACRE; REUEL L. GONÇALVES, BIOGENESIS BAGO SAÚDE ANIMAL; BERNARDO G. GASPERIN, UNIVERSIDADE FEDERAL DE PELOTAS; MONIQUE T. ROVANI, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL; EANES F. PAZ, ASSITÊNCIA TÉCNICA E EXTENSÃO RURAL; LEONARDO S. GOMES, Embrapa Rondônia; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO. |
Título: |
GnRH34 with or without estradiol cypionate in timed AI in Bos indicus beef cows. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Theriogenology, v. 209, p. 134-140, 2023. |
ISSN: |
0093-691X |
DOI: |
https://doi.org/10.1016/j.theriogenology.2023.06.026 |
Idioma: |
Inglês |
Conteúdo: |
Two experiments were performed to evaluate the effects of GnRH treatment on the fertility of suckled Nelore beef cows treated with an estradiol/progesterone (E2/P4)-based protocol for timed artificial insemination (TAI). Experiment 1 focused on determining the effects of estradiol cypionate (EC) on ovulation in TAI cows treated with GnRH 34 h after removal of the intravaginal P4 device (IPD). Suckled cows (n ¼ 26) were treated with 2 mg estradiol benzoate (EB) and IPD containing 1 g P4. After 8 days, IPDs were removed, and all cows were treated with 150 mg of d-cloprostenol (prostaglandin F2 alpha analog) and 300 IU of equine chorionic gonadotropin (eCG), then separated into two treatment groups consisting of cows who received 1) saline 0.9% i.m. (GnRH34 group) or 2) 0.6 mg i.m. of EC (EC-GnRH34 group). On day 9 (05:00 p.m.), all cows were given GnRH (10.5 mg of buserelin acetate) i.m. No differences were observed between the groups (P > 0.05) in the time of ovulation after IPD removal or in the proportion of cows ovulating. Experiment 2 focused on determining the effects of GnRH34 along with or in the absence of EC on day 8 on pregnancy per AI (P/AI) in postpartum beef cows. Cows (n ¼ 981) were treated similarly to those in Experiment 1, but an additional group, the EC-GnRH48 group, was included, in which cows received EC on day 8 whereas those that did not show estrus received GnRH at TAI. Thus, in this experiment, groups consisted of GnRH34 (n ¼ 322), EC-GnRH34 (n ¼ 335), and EC-GnRH48 (n ¼ 324). A higher rate of estrus expression was observed in cows treated with EC following IPD removal (EC-GnRH34: 69%, EC-GnRH48: 64.8%) than in cows in the GnRH34 group (45.6%). No difference in P/AI was observed between the treatment groups (P ¼ 0.45), but P/AI in cows in the EC-GnRH34 group (64.2%) tended to be greater (P ¼ 0.1) than in cows in the GnRH34 group (58%). In summary, although ovulation synchrony did not differ among the groups, P/AI in cows treated with EC and GnRH 34 h after IPD removal tended to be greater than in cows treated solely with GnRH; this was most likely due to a shorter proestrus/estrus period, considering the lower proportion of cows that displayed estrus in the GnRH34 group. Finally, given that P/AI did not differ between the EC-GnRH34 and EC-GnRH48 groups, our results suggest that, for cows not displaying estrus, administration of EC at the time of IPD removal followed by treatment with GnRH 48 h afterward represents the most cost-efficient TAI strategy for South American Zebu-based beef operations. MenosTwo experiments were performed to evaluate the effects of GnRH treatment on the fertility of suckled Nelore beef cows treated with an estradiol/progesterone (E2/P4)-based protocol for timed artificial insemination (TAI). Experiment 1 focused on determining the effects of estradiol cypionate (EC) on ovulation in TAI cows treated with GnRH 34 h after removal of the intravaginal P4 device (IPD). Suckled cows (n ¼ 26) were treated with 2 mg estradiol benzoate (EB) and IPD containing 1 g P4. After 8 days, IPDs were removed, and all cows were treated with 150 mg of d-cloprostenol (prostaglandin F2 alpha analog) and 300 IU of equine chorionic gonadotropin (eCG), then separated into two treatment groups consisting of cows who received 1) saline 0.9% i.m. (GnRH34 group) or 2) 0.6 mg i.m. of EC (EC-GnRH34 group). On day 9 (05:00 p.m.), all cows were given GnRH (10.5 mg of buserelin acetate) i.m. No differences were observed between the groups (P > 0.05) in the time of ovulation after IPD removal or in the proportion of cows ovulating. Experiment 2 focused on determining the effects of GnRH34 along with or in the absence of EC on day 8 on pregnancy per AI (P/AI) in postpartum beef cows. Cows (n ¼ 981) were treated similarly to those in Experiment 1, but an additional group, the EC-GnRH48 group, was included, in which cows received EC on day 8 whereas those that did not show estrus received GnRH at TAI. Thus, in this experiment, groups consisted of GnRH34 (n ¼ 322), EC-GnRH34 (n ¼ 335),... Mostrar Tudo |
Palavras-Chave: |
Amazônia Ocidental; Embrapa Rondôna; Western Amazon. |
Thesagro: |
Bos Indicus; Campo Experimental; Estradiol; Fertilidade Animal; Gado Nelore; Hormônio; Ovulação; Prenhez; Reprodução Animal; Vaca de Corte. |
Thesaurus NAL: |
Animal fertility; Animal reproduction; Beef cows; Demonstration farms; Hormones; Nellore; Ovulation; Pregnancy. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1154705/1/cpafro-18957.pdf
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Marc: |
LEADER 03947naa a2200505 a 4500 001 2154705 005 2023-07-18 008 2023 bl uuuu u00u1 u #d 022 $a0093-691X 024 7 $ahttps://doi.org/10.1016/j.theriogenology.2023.06.026$2DOI 100 1 $aSILVA, S. A. 245 $aGnRH34 with or without estradiol cypionate in timed AI in Bos indicus beef cows.$h[electronic resource] 260 $c2023 520 $aTwo experiments were performed to evaluate the effects of GnRH treatment on the fertility of suckled Nelore beef cows treated with an estradiol/progesterone (E2/P4)-based protocol for timed artificial insemination (TAI). Experiment 1 focused on determining the effects of estradiol cypionate (EC) on ovulation in TAI cows treated with GnRH 34 h after removal of the intravaginal P4 device (IPD). Suckled cows (n ¼ 26) were treated with 2 mg estradiol benzoate (EB) and IPD containing 1 g P4. After 8 days, IPDs were removed, and all cows were treated with 150 mg of d-cloprostenol (prostaglandin F2 alpha analog) and 300 IU of equine chorionic gonadotropin (eCG), then separated into two treatment groups consisting of cows who received 1) saline 0.9% i.m. (GnRH34 group) or 2) 0.6 mg i.m. of EC (EC-GnRH34 group). On day 9 (05:00 p.m.), all cows were given GnRH (10.5 mg of buserelin acetate) i.m. No differences were observed between the groups (P > 0.05) in the time of ovulation after IPD removal or in the proportion of cows ovulating. Experiment 2 focused on determining the effects of GnRH34 along with or in the absence of EC on day 8 on pregnancy per AI (P/AI) in postpartum beef cows. Cows (n ¼ 981) were treated similarly to those in Experiment 1, but an additional group, the EC-GnRH48 group, was included, in which cows received EC on day 8 whereas those that did not show estrus received GnRH at TAI. Thus, in this experiment, groups consisted of GnRH34 (n ¼ 322), EC-GnRH34 (n ¼ 335), and EC-GnRH48 (n ¼ 324). A higher rate of estrus expression was observed in cows treated with EC following IPD removal (EC-GnRH34: 69%, EC-GnRH48: 64.8%) than in cows in the GnRH34 group (45.6%). No difference in P/AI was observed between the treatment groups (P ¼ 0.45), but P/AI in cows in the EC-GnRH34 group (64.2%) tended to be greater (P ¼ 0.1) than in cows in the GnRH34 group (58%). In summary, although ovulation synchrony did not differ among the groups, P/AI in cows treated with EC and GnRH 34 h after IPD removal tended to be greater than in cows treated solely with GnRH; this was most likely due to a shorter proestrus/estrus period, considering the lower proportion of cows that displayed estrus in the GnRH34 group. Finally, given that P/AI did not differ between the EC-GnRH34 and EC-GnRH48 groups, our results suggest that, for cows not displaying estrus, administration of EC at the time of IPD removal followed by treatment with GnRH 48 h afterward represents the most cost-efficient TAI strategy for South American Zebu-based beef operations. 650 $aAnimal fertility 650 $aAnimal reproduction 650 $aBeef cows 650 $aDemonstration farms 650 $aHormones 650 $aNellore 650 $aOvulation 650 $aPregnancy 650 $aBos Indicus 650 $aCampo Experimental 650 $aEstradiol 650 $aFertilidade Animal 650 $aGado Nelore 650 $aHormônio 650 $aOvulação 650 $aPrenhez 650 $aReprodução Animal 650 $aVaca de Corte 653 $aAmazônia Ocidental 653 $aEmbrapa Rondôna 653 $aWestern Amazon 700 1 $aMONDADORI, R. G. 700 1 $aNOLETO, G. S. 700 1 $aBARBOSA, I. P. 700 1 $aGONÇALVES, R. L. 700 1 $aGASPERIN, B. G. 700 1 $aROVANI, M. T. 700 1 $aPAZ, E. F. 700 1 $aGOMES, L. S. 700 1 $aPFEIFER, L. F. M. 773 $tTheriogenology$gv. 209, p. 134-140, 2023.
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