Data/hora: 04/06/2024 16:49:09

Biblioteca(s): | Embrapa Arroz e Feijão.
Data corrente: | 20/11/2019
Data da última atualização: | 24/04/2020
Autoria: | PAIVA, B. A. R. de; WENDLAND, A.; TEIXEIRA, N. C.; FERREIRA, M. A. S. V.
Afiliação: | BRUNA ALICIA RAFAEL DE PAIVA, UNB; ADRIANE WENDLAND FERREIRA, CNPAF; NARA CRISTINA TEIXEIRA, bolsista CNPAF; MARISA A. S. V. FERREIRA, UNB.
Título: | Rapid detection of Xanthomonas citri pv. fuscans and Xanthomonas phaseoli pv. phaseoli in common bean by loop-mediated isothermal amplification.
Ano de publicação: | 2020
Fonte/Imprenta: | Plant Disease, v. 104, n. 1, p. 198-203, Jan. 2020.
ISSN: | 0191-2917
DOI: | 10.1094/PDIS-02-19-0325-RE
Idioma: | Inglês
Conteúdo: | A single loop-mediated isothermal amplification (LAMP) assay was developed for specific detection of both pathogens that cause bacterial blight in common bean, Xanthomonas phaseoli pv. phaseoli (Xpp) and Xanthomonas citri pv. fuscans (Xcf). The objective was to provide a simple, easy-to-use, specific, and sensitive method to investigate the presence of one or both pathogens in plant material and seeds for routine diagnosis. The detection limits for both pathogens were 10 CFU/ml for cell suspensions and 1 fg of DNA, whereas in conventional PCR, the primers detected up to 105 CFU/ml and 1 ng of DNA. Specificity was confirmed by testing DNA from bean leaves, other Xanthomonas species, common fungal and bacterial bean pathogens, and bacteria from the leaf microbiota. The method was tested with bean leaves inoculated with Xpp, and the pathogen could be detected from 4 h up to 15 days postinoculation, even before disease symptoms were visible. When the method was applied to bacterium detection (Xpp or Xcf) in seed lots from infected plants, the bacterium detection rate was 100% (24 of 24). The pathogens were detected in seeds incubated for just 1 h in saline solution (0.85%), reducing the time needed for bacterium detection. The LAMP assay could be useful as a tool in bean bacterial blight management. Rapid and sensitive detection of bacteria in bean seed lots would reduce the risks of planting highly contaminated seeds in environments favorable to blight multiplication.
Thesagro: | Bactéria; Doença de Planta; Feijão; Phaseolus Vulgaris; Xanthomonas Phaseoli.
Thesaurus Nal: | Bacterial diseases of plants; Beans; Fields; Loop-mediated isothermal amplification; Oilseeds; Pathogen identification; Plant diseases and disorders.
Categoria do assunto: | H Saúde e Patologia
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (35668-1) - UPC - AP - DD - 2020 -  - 2020 - DIS