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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
18/04/2023 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VASQUEZ, D. D. N.; PINHEIRO, D. H.; TEIXEIRA, L. A.; MOREIRA-PINTO, C. E.; MACEDO, L. L. P. de; SALLES-FILHO, A. L. O.; SILVA, M. C. M. da; LOURENCO, I. T.; MORGANTE, C. V.; SILVA, L. P. da; SA, M. F. G. de. |
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Afiliação: |
DANIEL D. N. VASQUEZ, Catholic University of Brasília; DANIELE H. PINHEIRO; LAYS A. TEIXEIRA, Catholic University of Brasília; CLIDIA E. MOREIRA-PINTO; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; ALVARO L. O. SALLES-FILHO, Federal University of Paraná; MARIA CRISTINA MATTAR DA SILVA, Cenargen; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; LUCIANO PAULINO DA SILVA, Cenargen; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Simultaneous silencing of juvenile hormone metabolism genes through RNAi interrupts metamorphosis in the cotton boll weevil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Frontiers in Molecular Biosciences, v. 10, 2023. |
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DOI: |
https://doi.org/10.3389/fmolb.2023.1073721 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Leonardo L. P. Macedo; Maria C. M. Silva; Isabela T. Lourenço-Tessutti; Luciano P. Silva; Maria F. Grossi-de-Sa. |
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Conteúdo: |
The cotton boll weevil (CBW) (Anthonomus grandis) is one of the major insect pests of cotton in Brazil. Currently, CBW control is mainly achieved by insecticide application, which is costly and insufficient to ensure effective crop protection. RNA interference (RNAi) has been used in gene function analysis and the development of insect control methods. However, some insect species respond poorly to RNAi, limiting the widespread application of this approach. Therefore, nanoparticles have been explored as an option to increase RNAi efficiency in recalcitrant insects. Herein, we investigated the potential of chitosan?tripolyphosphate (CS-TPP) and polyethylenimine (PEI) nanoparticles as a dsRNA carrier system to improve RNAi efficiency in the CBW. Different formulations of the nanoparticles with dsRNAs targeting genes associated with juvenile hormone metabolism, such as juvenile hormone diol kinase (JHDK), juvenile hormone epoxide hydrolase (JHEH), and methyl farnesoate hydrolase (MFE), were tested. The formulations were delivered to CBW larvae through injection (0.05?2 ?g), and the expression of the target genes was evaluated using RT-qPCR. PEI nanoparticles increased targeted gene silencing compared with naked dsRNAs (up to 80%), whereas CS-TPP-dsRNA nanoparticles decreased gene silencing (0%?20%) or led to the same level of gene silencing as the naked dsRNAs (up to 50%). We next evaluated the effects of targeting a single gene orsimultaneously targeting two genes via the injection of naked dsRNAs or dsRNAs complexed with PEI (500 ng) on CBW survival and phenotypes. Overall, the gene expression analysis showed that the treatments with PEI targeting either a single gene or multiple genes induced greater gene silencing than naked dsRNA (~60%). In addition, the injection of dsJHEH/JHDK, either naked or complexed with PEI, significantly affected CBW survival (18% for PEI nanoparticles and 47% for naked dsRNA) and metamorphosis. Phenotypic alterations, such as uncompleted pupation or malformed pupae, suggested that JHEH and JHDK are involved in developmental regulation. Moreover, CBW larvae treated with dsJHEH/JHDK + PEI (1,000 ng/g) exhibited significantly lower survival rate (55%) than those that MenosThe cotton boll weevil (CBW) (Anthonomus grandis) is one of the major insect pests of cotton in Brazil. Currently, CBW control is mainly achieved by insecticide application, which is costly and insufficient to ensure effective crop protection. RNA interference (RNAi) has been used in gene function analysis and the development of insect control methods. However, some insect species respond poorly to RNAi, limiting the widespread application of this approach. Therefore, nanoparticles have been explored as an option to increase RNAi efficiency in recalcitrant insects. Herein, we investigated the potential of chitosan?tripolyphosphate (CS-TPP) and polyethylenimine (PEI) nanoparticles as a dsRNA carrier system to improve RNAi efficiency in the CBW. Different formulations of the nanoparticles with dsRNAs targeting genes associated with juvenile hormone metabolism, such as juvenile hormone diol kinase (JHDK), juvenile hormone epoxide hydrolase (JHEH), and methyl farnesoate hydrolase (MFE), were tested. The formulations were delivered to CBW larvae through injection (0.05?2 ?g), and the expression of the target genes was evaluated using RT-qPCR. PEI nanoparticles increased targeted gene silencing compared with naked dsRNAs (up to 80%), whereas CS-TPP-dsRNA nanoparticles decreased gene silencing (0%?20%) or led to the same level of gene silencing as the naked dsRNAs (up to 50%). We next evaluated the effects of targeting a single gene orsimultaneously targeting two genes via the inj... Mostrar Tudo |
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Palavras-Chave: |
Bicudo do algodoeiro; Juvenile diol kinase; Juvenile hormone epoxide hydrolase; Polyethylenimine; Pragas do algodoeiro. |
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Thesagro: |
Algodão; Anthonomus Grandis; Inseto; Praga. |
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Thesaurus Nal: |
Chitosan; Cotton; Insect control; Insect pests. |
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Categoria do assunto: |
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O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1153217/1/fmolb-10-1073721.pdf
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Marc: |
LEADER 03612naa a2200421 a 4500
001 2153271
005 2023-05-25
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fmolb.2023.1073721$2DOI
100 1 $aVASQUEZ, D. D. N.
245 $aSimultaneous silencing of juvenile hormone metabolism genes through RNAi interrupts metamorphosis in the cotton boll weevil.$h[electronic resource]
260 $c2023
500 $aNa publicação: Leonardo L. P. Macedo; Maria C. M. Silva; Isabela T. Lourenço-Tessutti; Luciano P. Silva; Maria F. Grossi-de-Sa.
520 $aThe cotton boll weevil (CBW) (Anthonomus grandis) is one of the major insect pests of cotton in Brazil. Currently, CBW control is mainly achieved by insecticide application, which is costly and insufficient to ensure effective crop protection. RNA interference (RNAi) has been used in gene function analysis and the development of insect control methods. However, some insect species respond poorly to RNAi, limiting the widespread application of this approach. Therefore, nanoparticles have been explored as an option to increase RNAi efficiency in recalcitrant insects. Herein, we investigated the potential of chitosan?tripolyphosphate (CS-TPP) and polyethylenimine (PEI) nanoparticles as a dsRNA carrier system to improve RNAi efficiency in the CBW. Different formulations of the nanoparticles with dsRNAs targeting genes associated with juvenile hormone metabolism, such as juvenile hormone diol kinase (JHDK), juvenile hormone epoxide hydrolase (JHEH), and methyl farnesoate hydrolase (MFE), were tested. The formulations were delivered to CBW larvae through injection (0.05?2 ?g), and the expression of the target genes was evaluated using RT-qPCR. PEI nanoparticles increased targeted gene silencing compared with naked dsRNAs (up to 80%), whereas CS-TPP-dsRNA nanoparticles decreased gene silencing (0%?20%) or led to the same level of gene silencing as the naked dsRNAs (up to 50%). We next evaluated the effects of targeting a single gene orsimultaneously targeting two genes via the injection of naked dsRNAs or dsRNAs complexed with PEI (500 ng) on CBW survival and phenotypes. Overall, the gene expression analysis showed that the treatments with PEI targeting either a single gene or multiple genes induced greater gene silencing than naked dsRNA (~60%). In addition, the injection of dsJHEH/JHDK, either naked or complexed with PEI, significantly affected CBW survival (18% for PEI nanoparticles and 47% for naked dsRNA) and metamorphosis. Phenotypic alterations, such as uncompleted pupation or malformed pupae, suggested that JHEH and JHDK are involved in developmental regulation. Moreover, CBW larvae treated with dsJHEH/JHDK + PEI (1,000 ng/g) exhibited significantly lower survival rate (55%) than those that
650 $aChitosan
650 $aCotton
650 $aInsect control
650 $aInsect pests
650 $aAlgodão
650 $aAnthonomus Grandis
650 $aInseto
650 $aPraga
653 $aBicudo do algodoeiro
653 $aJuvenile diol kinase
653 $aJuvenile hormone epoxide hydrolase
653 $aPolyethylenimine
653 $aPragas do algodoeiro
700 1 $aPINHEIRO, D. H.
700 1 $aTEIXEIRA, L. A.
700 1 $aMOREIRA-PINTO, C. E.
700 1 $aMACEDO, L. L. P. de
700 1 $aSALLES-FILHO, A. L. O.
700 1 $aSILVA, M. C. M. da
700 1 $aLOURENCO, I. T.
700 1 $aMORGANTE, C. V.
700 1 $aSILVA, L. P. da
700 1 $aSA, M. F. G. de
773 $tFrontiers in Molecular Biosciences$gv. 10, 2023.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
04/02/2009 |
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Data da última atualização: |
04/02/2009 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
WAMBACH, X. F.; LUDKE, M. do C. M. M.; COSTA, W. de M.; ARANDAS, J. K. G.; ALENCAR, J. D. T. de A.; LUDKE, J. V.; PORTO NETO, F. de F. |
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Afiliação: |
Xélen Faria Wambach, UFRPE; Maria do Carmo Mohaupt Marques Ludke, Universidade Federal Rural de Pernambuco; Waleska de Melo Costa, Universidade Federal Rural de Pernambuco; Janaina Kelli Gomes Arandas, UFRPE; Joana D'arc Timoteo de Alencar, UFRPE; Jorge Vitor Ludke, Embrapa Suínos e Aves; Fernando de Figueiredo Porto Neto, UFRPE. |
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Título: |
Composição nutricional de ingredientes alternativos para aves, suínos e peixes: farelo de sisal. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: JORNADA DE ENSINO, PESQUISA E EXTENSÃO DA UFRPE, 8., 2008, Recife. Anais. Recife: Universidade Federal de Pernambuco, 2008. |
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Idioma: |
Português |
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Notas: |
Projeto/Plano de Ação: 16.00.30004-00. Publicado em CD-ROM. JEPEX 2008. |
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Palavras-Chave: |
Alimentos alternativos; Ave; Farelo de sisal; Peixes; Suínos. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00873naa a2200253 a 4500
001 1444129
005 2009-02-04
008 2008 bl --- 0-- u #d
100 1 $aWAMBACH, X. F.
245 $aComposição nutricional de ingredientes alternativos para aves, suínos e peixes$bfarelo de sisal.
260 $c2008
500 $aProjeto/Plano de Ação: 16.00.30004-00. Publicado em CD-ROM. JEPEX 2008.
653 $aAlimentos alternativos
653 $aAve
653 $aFarelo de sisal
653 $aPeixes
653 $aSuínos
700 1 $aLUDKE, M. do C. M. M.
700 1 $aCOSTA, W. de M.
700 1 $aARANDAS, J. K. G.
700 1 $aALENCAR, J. D. T. de A.
700 1 $aLUDKE, J. V.
700 1 $aPORTO NETO, F. de F.
773 $tIn: JORNADA DE ENSINO, PESQUISA E EXTENSÃO DA UFRPE, 8., 2008, Recife. Anais. Recife: Universidade Federal de Pernambuco, 2008.
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Embrapa Suínos e Aves (CNPSA) |
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