Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
10/06/1999 |
Data da última atualização: |
10/06/1999 |
Autoria: |
PEDROSA, F. O.; TEIXEIRA, K. R. dos S.; MACHADO, I. M. P.; STEFFENS, M. B. R.; KLASSEN, G.; BENELLI, E. M.; MACHADO, H. B.; FUNAYAMA, S.; RIGO, L. U.; ISHIDA, M. L.; YATES, M. G.; SOUZA, E. M. |
Título: |
Structural organization and regulation of the Nif genes of Herbaspirillum seropedicae. |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
Soil Biology and Biochemistry, Oxford, v. 29, n. 5/6, p. 843-846, 1997. |
Idioma: |
Inglês |
Conteúdo: |
The promoter regions of nifA and nifH genes of Herbaspirillum seropedicae have been isolated and investigated, and the nifA promoter has been characterized in detail. Both regions contain a -24/-12 type promoter and nifA-binding sites; the nifA promoter also has an NtrC-binding site. The nifA expression is activated by NtrC and repressed by ammonium ions but not by oxygen. When the NtrC-binding site was deleted nifA expression was NifA-dependent. The native NifA protein of H. seropedicae was unable to activate nifH expression in Escherichia coli whereas a truncated protein, lacking the N-terminal domain was able to activate nifH expression in the absence of oxygen and presence of iron, indicating that the N-terminal domain regulates NifA activity. The truncated protein also activates nifH in Azospirillum brasilense in the presence or absence of ammonium ions. This suggests that the N-terminal domain senses the nitrogen status of the cell. |
Palavras-Chave: |
BNF; FBN; Fixação biologica de nitrogênio; Nitrogen fixing bacteria. |
Thesagro: |
Gene; Proteína. |
Thesaurus Nal: |
genes; proteins. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01903naa a2200349 a 4500 001 1621090 005 1999-06-10 008 1997 bl uuuu u00u1 u #d 100 1 $aPEDROSA, F. O. 245 $aStructural organization and regulation of the Nif genes of Herbaspirillum seropedicae. 260 $c1997 520 $aThe promoter regions of nifA and nifH genes of Herbaspirillum seropedicae have been isolated and investigated, and the nifA promoter has been characterized in detail. Both regions contain a -24/-12 type promoter and nifA-binding sites; the nifA promoter also has an NtrC-binding site. The nifA expression is activated by NtrC and repressed by ammonium ions but not by oxygen. When the NtrC-binding site was deleted nifA expression was NifA-dependent. The native NifA protein of H. seropedicae was unable to activate nifH expression in Escherichia coli whereas a truncated protein, lacking the N-terminal domain was able to activate nifH expression in the absence of oxygen and presence of iron, indicating that the N-terminal domain regulates NifA activity. The truncated protein also activates nifH in Azospirillum brasilense in the presence or absence of ammonium ions. This suggests that the N-terminal domain senses the nitrogen status of the cell. 650 $agenes 650 $aproteins 650 $aGene 650 $aProteína 653 $aBNF 653 $aFBN 653 $aFixação biologica de nitrogênio 653 $aNitrogen fixing bacteria 700 1 $aTEIXEIRA, K. R. dos S. 700 1 $aMACHADO, I. M. P. 700 1 $aSTEFFENS, M. B. R. 700 1 $aKLASSEN, G. 700 1 $aBENELLI, E. M. 700 1 $aMACHADO, H. B. 700 1 $aFUNAYAMA, S. 700 1 $aRIGO, L. U. 700 1 $aISHIDA, M. L. 700 1 $aYATES, M. G. 700 1 $aSOUZA, E. M. 773 $tSoil Biology and Biochemistry, Oxford$gv. 29, n. 5/6, p. 843-846, 1997.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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