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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
07/02/2017 |
Data da última atualização: |
30/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GUIMARÃES-CESTARO, L.; SERRÃO, J. E.; MESSAGE, D.; MARTINS, M. F.; TEIXEIRA, É. W. |
Afiliação: |
Lubiane Guimarães-Cestaro, UFV; José Eduardo Serrão, UFV; Dejair Message, UFERSA; MARTA FONSECA MARTINS, CNPGL; Érica Weinstein Teixeira, APTA-SAA. |
Título: |
Simultaneous detection of Nosema spp., Ascosphaera apis and Paenibacillus larvae in honey bee products. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Journal of Hymenoptera Research, n. 49, p. 43-50, 2016. |
Idioma: |
Inglês |
Conteúdo: |
Abstract Honey bees are responsible for pollinating many native and cultivated plant species. These insects can be affected by many pathogens, including fungi and bacteria, both of which can form spores that are easily dispersed within the colony by means of the stored products, among other routes. The objective of this study was to develop a method to detect spores of the honey bee pathogens Nosema apis, Nosema ceranae, Ascosphaera apis and Paenibacillus larvae in samples of honey, bee pollen and royal jelly. The method was standardized for each product individually, and then analyzed by monoplex and multiplex PCR, which showed the same detection thresholds: 1.25 spores/mL of honey for N. ceranae; 7.5 spores/mL of honey for A. apis; and 0.4 spore/mL of honey for P. larvae, respectively. The standardized technique was effective and rapid for the detection of these pathogens in bee products and can be used for the establishment of official methods of sanitary control of bee products, considering the growing national and international trade of these products and the movement or migration of colonies between regions. |
Palavras-Chave: |
Bee pathogens; Detection of spores. |
Thesagro: |
Apis Mellifera. |
Thesaurus Nal: |
honey; pollen; royal jelly. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154930/1/Cnpgl-2016-JHymenopRes-Simultaneous.pdf
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Marc: |
LEADER 01838naa a2200241 a 4500 001 2062982 005 2023-01-30 008 2016 bl uuuu u00u1 u #d 100 1 $aGUIMARÃES-CESTARO, L. 245 $aSimultaneous detection of Nosema spp., Ascosphaera apis and Paenibacillus larvae in honey bee products.$h[electronic resource] 260 $c2016 520 $aAbstract Honey bees are responsible for pollinating many native and cultivated plant species. These insects can be affected by many pathogens, including fungi and bacteria, both of which can form spores that are easily dispersed within the colony by means of the stored products, among other routes. The objective of this study was to develop a method to detect spores of the honey bee pathogens Nosema apis, Nosema ceranae, Ascosphaera apis and Paenibacillus larvae in samples of honey, bee pollen and royal jelly. The method was standardized for each product individually, and then analyzed by monoplex and multiplex PCR, which showed the same detection thresholds: 1.25 spores/mL of honey for N. ceranae; 7.5 spores/mL of honey for A. apis; and 0.4 spore/mL of honey for P. larvae, respectively. The standardized technique was effective and rapid for the detection of these pathogens in bee products and can be used for the establishment of official methods of sanitary control of bee products, considering the growing national and international trade of these products and the movement or migration of colonies between regions. 650 $ahoney 650 $apollen 650 $aroyal jelly 650 $aApis Mellifera 653 $aBee pathogens 653 $aDetection of spores 700 1 $aSERRÃO, J. E. 700 1 $aMESSAGE, D. 700 1 $aMARTINS, M. F. 700 1 $aTEIXEIRA, É. W. 773 $tJournal of Hymenoptera Research$gn. 49, p. 43-50, 2016.
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Embrapa Gado de Leite (CNPGL) |
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Registro Completo
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
24/09/2014 |
Data da última atualização: |
16/12/2014 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
PILON, L.; SPRICIGO, P. C.; BRITTO, D.; ASSIS, O. B. G. de; CALBO, A. G.; FERRAUDO, A. S.; FERREIRA, M. D. |
Afiliação: |
LUCIMEIRE PILON, CNPH; POLIANA CRISTINA SPRICIGO, UNIVERSIDADE FEDERAL DE SÃO CARLOS; DOUGLAS DE BRITTO; ODILIO BENEDITO GARRIDO DE ASSIS, CNPDIA; ADONAI GIMENEZ CALBO, CNPDIA; ANTONIO SERGIO FERRAUDO, UNIVERSIDADE DE SÃO PAULO; MARCOS DAVID FERREIRA, CNPDIA. |
Título: |
Effects of antibrowning solution and chitosan-based edible coating on the quality of fresh-cut apple. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
International Journal of Postharvest Technology and Innovation , [S. l.] v. 207-208, p. 151-164, 2013. |
ISSN: |
1744-7550 |
Idioma: |
Inglês |
Palavras-Chave: |
Apple. |
Thesaurus NAL: |
chitosan; citric acid; firmness. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/109031/1/Pilon-et-al-2013.pdf
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Marc: |
LEADER 00743naa a2200241 a 4500 001 1995812 005 2014-12-16 008 2013 bl uuuu u00u1 u #d 022 $a1744-7550 100 1 $aPILON, L. 245 $aEffects of antibrowning solution and chitosan-based edible coating on the quality of fresh-cut apple.$h[electronic resource] 260 $c2013 650 $achitosan 650 $acitric acid 650 $afirmness 653 $aApple 700 1 $aSPRICIGO, P. C. 700 1 $aBRITTO, D. 700 1 $aASSIS, O. B. G. de 700 1 $aCALBO, A. G. 700 1 $aFERRAUDO, A. S. 700 1 $aFERREIRA, M. D. 773 $tInternational Journal of Postharvest Technology and Innovation , [S. l.]$gv. 207-208, p. 151-164, 2013.
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