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| Registros recuperados : 44 | |
| 21. |  | ZANUNCIO, J. C.; TAVARES, W. de S.; RAMALHO, F. de S.; LEITE, G. L. D.; SERRÃO, J. E. Sarsina violascens spatial and temporal distributions affected by native getetation strips in eucalyptus plantationsl Pesquisa Agropecuária Brasileira, Brasília, DF, v. 51, n. 6, p. 703-709, jun. 2016. Título em português: Distribuição espacial e temporal de Sarsina violascens influenciada por faixas de vegetação nativa em plantações de eucalipto.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 22. | | NASCIMENTO, C. E. de S.; TABARELLI, M.; SILVA, C. A. D. da; LEAL, I. R.; TAVARES, W. de S.; SERRÃO, J. E.; ZANUNCIO, J. C. The introduced tree Prosopis juliflora is a serious threat to native species of the Brazilian Caatinga vegetation. Science of the Total Environment, v. 481, p. 108-113, 2014.| Biblioteca(s): Embrapa Semiárido. |
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| 23. | | SILVA, R. B. da; CRUZ, I.; FIGUEIREDO, M. de L. C.; TAVARES, W. de S.; FERREIRA, C. F.; REDOAN, A. C. Aspectos reprodutivos de Eriopis connexa (Germar) (Coleoptera: Coccinellidae) com ninfas de Schizaphis graminum (Rondani) (Hemiptera: Aphididae). In: CONGRESSO NACIONAL DE MILHO E SORGO, 28.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA DO CARTUCHO, 4., 2010, Goiânia. Potencialidades, desafios e sustentabilidade: resumos expandidos... Sete Lagoas: ABMS, 2010. 1 CD-ROM.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 24. | | ZANUNCIO, J. C.; MATOS NETO, F. da C.; TAVARES, W. de S.; CRUZ, I.; LEITE, G. L. D.; SERRÃO, J. E. Functional and numerical responses and reproduction of Campoletis flavicincta parasitizing Spodoptera frugiperda caterpillars. Acta Scientiarum. Agronomy, Maringá, v. 35, n. 4, p. 419-426, out./dez. 2013.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 25. | | CRUZ, R. A.; ZANUNCIO, J. C.; LACERDA, M. C.; WILCKEN, C. F.; FERNANDES, F. L.; TAVARES, W. de S.; SOARES, M. A.; SEDIYAMA, C. S. Side-effects of pesticides on the generalist endoparasitoid Palmistichus elaeisis (Hymenoptera: Eulophidae). Scientific Reports, v. 7, n. 10064, Aug. 2017.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 26. | | TAVARES, W. DE S.; WILCKEN, C. F.; RAMALHO, F. de S.; SOARES, M. A.; FERNANDES, F. L.; SERRÃO, J. E.; ZANUNCIO, J. C. Discovery of the first Aximopsis (Hymenoptera: Eurytomidae) parasitoid of pidoptera in Brazil and notes on its biology. Florida Entomologist, v. 98, n. 4, p. 1077-1080, 2015.| Biblioteca(s): Embrapa Algodão. |
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| 27. | | SILVA, R. B. da; CRUZ, I.; FIGUEIREDO, M. de L. C.; TAVARES, W. de S.; SERRÃO, J. E.; ZANUNCIO, J. C. Development and reproduction of Olla v-nigrum (Coleoptera: Coccinellidae) fed Anagasta kuehniella (Lepidoptera: Pyralidae) eggs supplemented with an artificial diet. Florida Entomologist, Gainesville, v. 96, n. 3, p. 850-858, Sept. 2013.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 28. | | SILVA, R. B. da; CRUZ, I.; FIGUEIREDO, M. de L. C.; TAVARES, W. de S.; REDOAN, A. C.; ZANUNCIO, J. C. Development of Eriopis connexa (Germar) (Coleoptera: coccinellidae) with eggs of Spodoptera frugiperda (J. E. Smith) (Lepidoptera: noctuidae). In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. 1 CD-ROM.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 29. | | SOUZA JÚNIOR, A. A. de; GRAZZIOTTI, G. H.; PETACCI, F.; FREITAS, S. de S.; PARENTE, L. M. L.; LIÃO, L. M.; TAVARES, W. de S; CRUZ, I. Atividades inseticida e repelente de ar-turmerona isolada de rizomas de Curcuma longa L. In: REUNIÃO ANUAL SOCIEDADE BRASILEIRA DE QUÍMICA, 34., 2011, Florianópolis. A química para um mundo melhor. Florianópolis: SBQ, 2011.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 30. | | MASSON, M. V.; TAVARES, W. de S.; ALVES, J. M.; FERREIRA-FILHO, P. J.; BARBOSA, L. R.; WILCKEN, C. F.; ZANUNCIO, J. C. Bioecological aspects of the common black field cricket, Gryllus assimilis(Orthoptera: Gryllidae) in the laboratory and in Eucalyptus (Myrtaceae) plantations. Journal of Orthoptera Research, v. 29, n. 1, p. 83-89, 2020.| Biblioteca(s): Embrapa Florestas. |
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| 31. | | SILVA, R. B. da; CRUZ, I.; FIGUEIREDO, M. de L. C.; TAVARES, W. de S.; REDOAN, A. C.; ZANUNCIO, J. C. Biological aspects of Eriopis connexa (Germar) (Coleoptera: Coccinellidae) with different insect pest F the maize and sorghum agroecosystems. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. 1 CD-ROM.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 32. | | PEREIRA, A. I. de A.; CANTUÁRIO, F. S. de; SOUSA, P. V. de; TAVARES, W. de S.; MEDEIROS, M. G. H. de; MELO, W. F. de. Especificidade de Disonycha (Chrysomelidade) em plantas de caruru e genótipos de batata-doce. Horticultura Brasileira, Brasília, DF, v. 29, n. 2, jul. 2011. p. 1100-1107. CD-ROM. Suplemento. Trabalho apresentado no 51. Congresso Brasileiro de Olericultura, Viçosa, MG.| Biblioteca(s): Embrapa Hortaliças. |
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| 33. | | TAVARES, W. de S.; MASSON, M. V.; FERREIRA-FILHO, P. J.; BARBOSA, L. R.; SILVA, W. M.; WILCKEN, C. F.; ZANUNCIO, J. C. Parasitism of Lepidopteran Defoliators of Urban Plants by Palmistichus elaeisis (Hymenoptera: Eulophidae). Florida Entomologist, v. 101, n. 3, p. 453-457. Sept. 2018.| Biblioteca(s): Embrapa Florestas. |
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| 34. | | SILVA, R. B. da; CRUZ, I.; FIGUEIREDO, M. de L. C.; REDOAN, A. C. M.; SHIMBORI, E. M.; TAVARES, W. de S.; DIAS, A. M. P. M. Natural enemies recovered from Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) larvae infesting the cartridge, ear and stem of corn plants under conventional and organic farming systems in Brazil. Anais da Academia Brasileira de Ciências, v. 95, n. 4, e20200042, 2023.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 35. | | GUERRA, A. M. N. de M.; PEREIRA, A. I. A.; SILVA, C. A. D. da; TAVARES, W. de S.; RAMALHO, F. de S.; ZANUNCIO, J. C. Registro e biologia de halysidota pearsoni (lepidoptera: arctiidae) em plantas de gossypium hirsutum (malvaceae) em Viçosa, Minas Gerais, Brasil. In: CONGRESSO BRASILEIRO DO ALGODÃO, 7., 2009, Foz do Iguaçu. Sustentabilidade da cotonicultura brasileira e expansão dos mercados: anais. Campina Grande: Embrapa Algodão, 2009. 1 CD-ROM| Biblioteca(s): Embrapa Algodão. |
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| 36. | | GUERRA, A. M. N. de M.; PEREIRA, A. I. A.; COSTA, M. A.; SILVEIRA, R. D.; TAVARES, W. de S.; CRUZ, I. Seletividade do predador eriopis connexa (coleoptera: coccinelidae) e de sua presa, spodoptera frugiperda (lepidoptera: noctuidae), avaliada através do uso de produtos naturais. In: CONGRESSO BRASILEIRO DO ALGODÃO, 7., 2009, Foz do Iguaçu. Sustentabilidade da cotonicultura brasileira e expansão dos mercados: anais. Campina Grande: Embrapa Algodão, 2009. 1 CD-ROM| Biblioteca(s): Embrapa Algodão. |
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| 37. | | GUERRA, A. M. N. de M.; PEREIRA, A. I. A.; COSTA, M. A.; SILVEIRA, R. D.; TAVARES, W. de S.; CRUZ, I. Seletividade do predador Eriopis connexa (Coleoptera: coccinelidae) e de sua presa, Spodoptera frugiperda (Lepidoptera: noctuidae), avaliada através do uso de produtos naturais. In: CONGRESSO BRASILEIRO DO ALGODÃO, 7., 2009, Foz do Iguaçu. Sustentabilidade da cotonicultura brasileira e expansão dos mercados: anais. Campina Grande: Embrapa Algodão, 2009. 1 CD-ROM.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 38. | | MASSON, M. V.; TAVARES, W. de S.; LOPES, F. de A.; SOUZA, A. R. de; FERREIRA-FILHO, P. J.; BARBOSA, L. R.; WILCKEN, C. F.; ZANUNCIO, J. C. Selitrichodes neseri (Hymenoptera: Eulophidae) recovered from Leptocybe invasa (Hymenoptera: Eulophidae) galls after initial release on Eucalyptus (Myrtaceae) in Brazil, and data on its biology. Florida Entomologist, v. 100, n. 3, p. 589-593, Sept. 2017.| Biblioteca(s): Embrapa Florestas. |
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| 39. | | SILVA, R. B.; CRUZ, I.; FIGUEIREDO, M. de L. C.; TAVARES, W. de S.; FERREIRA, C. F.; SOUZA, L. P. S. P. Potencial reprodutivo de Hippodamia convergens Guérin- Méneville (Coleoptera: Coccinellidae) com Schizaphis graminum (Rondani) (Hemiptera: Aphididae). In: CONGRESSO NACIONAL DE MILHO E SORGO, 28.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA DO CARTUCHO, 4., 2010, Goiânia. Potencialidades, desafios e sustentabilidade: resumos expandidos... Sete Lagoas: ABMS, 2010. 1 CD-ROM.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 40. | | TAVARES, W. de S.; CRUZ, I.; PETACCI, F.; ASSIS JÚNIOR, S. L. de; FREITAS, S. de S.; ZANUNCIO, J. C.; SERRÃO, J. E. Potential use of Asteraceae extracts to control Spodoptera frugiperda (Lepidoptera: Noctuidae) and selectivity to their parasitoids Trichogramma pretiosum (Hymenoptera: Trichogrammatidae) and Telenomus remus (Hymenoptera: Scelionidae). Industrial Crops and Products, v. 30, p. 384-388, 2009.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 44 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
21/02/2019 |
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Data da última atualização: |
13/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SILVA, C. G. da; MARTINS, C. F.; CUMPA, H. C. B.; FONSECA NETO, A. M. da; CARDOSO, T. C.; FRANCO, M. M.; MENDONÇA, A. dos S.; LEME, L. de O.; BORGES, J. R. J.; MALAQUIAS, J. V.; BAO, S. N. |
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Afiliação: |
CAROLINA GONZALES DA SILVA, UNB; CARLOS FREDERICO MARTINS, CPAC; HEIDI CHRISTINA BESSLER CUMPA, CPAC; ALVARO MORAES DA FONSECA NETO, CPAC; TEREZA CRISTINA CARDOSO, UNESP; MAURICIO MACHAIM FRANCO, Cenargen; ANELISE DOS SANTOS MENDONÇA, UNIVERSIDADE DE UBERLÂNDIA; LIGIANE DE OLIVEIRA LEME, Cenargen; JOSÉ RENATO JUNQUEIRA BORGES, UNB; JUACI VITORIA MALAQUIAS, CPAC; SONIA NAIR BAO, UNB. |
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Título: |
Use of trichostatin A alters the expression of HDAC3 and KAT2 and improves in vitro development of bovine embryos cloned using less methylated mesenchymal stem cells. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Reproduction in Domestic Animals, v. 54, n. 2, p. 289-299, February 2019. |
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DOI: |
10.1111/rda.13360 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The aim of this work was to investigate the methylation and hydroxymethylation status of mesenchymal stem cells (MSC) from amniotic fluid (MSC-AF), adipose tissue (MSC-AT) and fibroblasts (FIB-control) and to verify the effect of trichostatin A (TSA) on gene expression and development of cloned bovine embryos produced using these cells. Characterization of MSC from two animals (BOV1 and BOV2) was performed by flow cytometry, immunophenotyping and analysis of cellular differentiation genes expression. The cells were used in the nuclear transfer in the absence or presence of 50 nM TSA for 20 hr in embryo culture. Expression of HDAC1, HDAC3 and KAT2A genes was measured in embryos by qRT-PCR. Methylation results showed difference between animals, with MSC from BOV2 demonstrating lower methylation rate than BOV1. Meanwhile, MSC-AF were less hydroxymethylated for both animals. MSC-AF from BOV2 produced 44.92 ± 8.88% of blastocysts when embryos were exposed to TSA and similar to embryo rate of MSC-AT also treated with TSA (37.96 ± 15.80%). However, when methylation was lower in FIB compared to MSC, as found in BOV1, the use of TSA was not sufficient to increase embryo production. MSC-AF embryos expressed less HDAC3 when treated with TSA, and expression of KAT2A was higher in embryos produced with all MSC and treated with TSA than embryos produced with FIB. The use of MSC less methylated and more hydroxymethylated in combination with embryo incubation with TSA can induce lower expression of HDAC3 and higher expression of KAT2A in the embryos and consequently improve bovine embryo production. MenosAbstract: The aim of this work was to investigate the methylation and hydroxymethylation status of mesenchymal stem cells (MSC) from amniotic fluid (MSC-AF), adipose tissue (MSC-AT) and fibroblasts (FIB-control) and to verify the effect of trichostatin A (TSA) on gene expression and development of cloned bovine embryos produced using these cells. Characterization of MSC from two animals (BOV1 and BOV2) was performed by flow cytometry, immunophenotyping and analysis of cellular differentiation genes expression. The cells were used in the nuclear transfer in the absence or presence of 50 nM TSA for 20 hr in embryo culture. Expression of HDAC1, HDAC3 and KAT2A genes was measured in embryos by qRT-PCR. Methylation results showed difference between animals, with MSC from BOV2 demonstrating lower methylation rate than BOV1. Meanwhile, MSC-AF were less hydroxymethylated for both animals. MSC-AF from BOV2 produced 44.92 ± 8.88% of blastocysts when embryos were exposed to TSA and similar to embryo rate of MSC-AT also treated with TSA (37.96 ± 15.80%). However, when methylation was lower in FIB compared to MSC, as found in BOV1, the use of TSA was not sufficient to increase embryo production. MSC-AF embryos expressed less HDAC3 when treated with TSA, and expression of KAT2A was higher in embryos produced with all MSC and treated with TSA than embryos produced with FIB. The use of MSC less methylated and more hydroxymethylated in combination with embryo incubation with TSA can induce l... Mostrar Tudo |
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Palavras-Chave: |
Acetilação de histona; Célula adiposa; Célula amniótica. |
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Thesagro: |
Bovino; Clonagem; DNA; Melhoramento Genético Animal; Metilação. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
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008 2019 bl uuuu u00u1 u #d
024 7 $a10.1111/rda.13360$2DOI
100 1 $aSILVA, C. G. da
245 $aUse of trichostatin A alters the expression of HDAC3 and KAT2 and improves in vitro development of bovine embryos cloned using less methylated mesenchymal stem cells.$h[electronic resource]
260 $c2019
520 $aAbstract: The aim of this work was to investigate the methylation and hydroxymethylation status of mesenchymal stem cells (MSC) from amniotic fluid (MSC-AF), adipose tissue (MSC-AT) and fibroblasts (FIB-control) and to verify the effect of trichostatin A (TSA) on gene expression and development of cloned bovine embryos produced using these cells. Characterization of MSC from two animals (BOV1 and BOV2) was performed by flow cytometry, immunophenotyping and analysis of cellular differentiation genes expression. The cells were used in the nuclear transfer in the absence or presence of 50 nM TSA for 20 hr in embryo culture. Expression of HDAC1, HDAC3 and KAT2A genes was measured in embryos by qRT-PCR. Methylation results showed difference between animals, with MSC from BOV2 demonstrating lower methylation rate than BOV1. Meanwhile, MSC-AF were less hydroxymethylated for both animals. MSC-AF from BOV2 produced 44.92 ± 8.88% of blastocysts when embryos were exposed to TSA and similar to embryo rate of MSC-AT also treated with TSA (37.96 ± 15.80%). However, when methylation was lower in FIB compared to MSC, as found in BOV1, the use of TSA was not sufficient to increase embryo production. MSC-AF embryos expressed less HDAC3 when treated with TSA, and expression of KAT2A was higher in embryos produced with all MSC and treated with TSA than embryos produced with FIB. The use of MSC less methylated and more hydroxymethylated in combination with embryo incubation with TSA can induce lower expression of HDAC3 and higher expression of KAT2A in the embryos and consequently improve bovine embryo production.
650 $aBovino
650 $aClonagem
650 $aDNA
650 $aMelhoramento Genético Animal
650 $aMetilação
653 $aAcetilação de histona
653 $aCélula adiposa
653 $aCélula amniótica
700 1 $aMARTINS, C. F.
700 1 $aCUMPA, H. C. B.
700 1 $aFONSECA NETO, A. M. da
700 1 $aCARDOSO, T. C.
700 1 $aFRANCO, M. M.
700 1 $aMENDONÇA, A. dos S.
700 1 $aLEME, L. de O.
700 1 $aBORGES, J. R. J.
700 1 $aMALAQUIAS, J. V.
700 1 $aBAO, S. N.
773 $tReproduction in Domestic Animals$gv. 54, n. 2, p. 289-299, February 2019.
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