|
|
Registros recuperados : 44 | |
6. | | TAVARES, W. de S.; GOUVEIA, N. L.; FREITAS, S. de; CRUZ, I. Identificação e flutuação populacional de Crisopideos (Neuroptera, Chrysopidae) associados à cultura orgânica de Crotolaria juncea (Leguminosae). In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: resumos... Viçosa: UFV, 2008. 1 CD-ROM. Biblioteca(s): Embrapa Milho e Sorgo. |
| |
8. | | MOREIRA, C. de O.; TAVARES, W. de S.; FONSECA, F. G.; CRUZ, I. Mortalidade de Spodoptera frugiperda (Lepidoptera, Noctuidae) e seletividade de Eriopis connexa (Coleoptera, Coccinellidae) com óleo de nim, extrato pirolenhoso e um inseticida químico sintético. In: CONGRESSO DE EXTENSÃO DA UFLA, 4.; FÓRUM REGIONAL DE EXTENSÃO, 1., 2009, Lavras. Anais. Lavras: UFLA, 2009. 1 CD-ROM. Biblioteca(s): Embrapa Milho e Sorgo. |
| |
10. | | QUEIROZ, D. L. de; TAVARES, W. de S.; ARAUJO, C. R. de; BURCKHARDT, D. New country, Brazilian states and host records of the eucalypt shoot psyllid Blastopsylla occidentalis Pesquisa Florestal Brasileira, Colombo, v. 38, e201701533, 2018. 4 p. Novos registros de países, estados brasileiros e hospedeiros para o psilídeo das ponteiras do eucalipto Blastopsylla occidentalis.
Pesquisa Florestal Brasileira, Colombo, v. 38, e201701533, 2018. 4 p.
Pesquisa Florestal Brasileira, v.... Biblioteca(s): Embrapa Agrobiologia. |
| |
11. | | TAVARES, W. de S.; FONSECA, F. G.; PETACCI, F.; FREITAS, S. S. de; CRUZ, I. Efeito em curto-prazo de produtos botânicos sobre Spodoptera frugiperda (Smith) (Lep., Noctuidae) e inimigos naturais. In: CONGRESSO DE EXTENSÃO DA UFLA, 4.; FÓRUM REGIONAL DE EXTENSÃO, 1., 2009, Lavras. Anais. Lavras: UFLA, 2009. 1 CD-ROM. Biblioteca(s): Embrapa Milho e Sorgo. |
| |
12. | | FONSECA, F. G.; TAVARES, W. de S.; PETACCI, F.; FREITAS, S. S. de; CRUZ, I. Efeito deletério e deterrente de extratos de plantas sobre Spodoptera frugiperda Smith (Lepidoptera, Noctuidae). In: CONGRESSO DE EXTENSÃO DA UFLA, 4.; FÓRUM REGIONAL DE EXTENSÃO, 1., 2009, Lavras. Anais. Lavras: UFLA, 2009. 1 CD-ROM. Biblioteca(s): Embrapa Milho e Sorgo. |
| |
13. | | MOREIRA, C. de O.; TAVARES, W. de S.; FONSECA, F. G.; ASSIS JÚNIOR, S. L. de; CRUZ, I. Aspectos econômicos para construção de uma biofábrica de Trichogramma visando controlar Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) no milho. In: CONGRESSO DE EXTENSÃO DA UFLA, 4.; FÓRUM REGIONAL DE EXTENSÃO, 1., 2009, Lavras. Anais. Lavras: UFLA, 2009. 1 CD-ROM. Biblioteca(s): Embrapa Milho e Sorgo. |
| |
14. | | TAVARES, W. de S.; ZANUNCIO, J. C.; SILVA, R. B. da; PETACCI, F.; CRUZ, I.; SERRÃO, J. E. Extracts and fractions of cerrado plants are selective for the parasitoid Palmistichus elaeisis Delvare & La Salle, 1993 (Hymenoptera: eulophidae). In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. 1 CD-ROM. Biblioteca(s): Embrapa Milho e Sorgo. |
| |
15. | | SILVA, R. B.; CRUZ, I.; FIGUEIREDO, M. de L. C.; TAVARES, W. de S.; FERREIRA, C. F.; REDOAN, A. C. Fecundidade e fertilidade de Eriopis connexa (Germar) (Coleoptera: Coccinellidae) com ovos de Diatraea saccharalis Fabr. (Lepidoptera: Crambidae).; In: CONGRESSO NACIONAL DE MILHO E SORGO, 28.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA DO CARTUCHO, 4., 2010, Goiânia. Potencialidades, desafios e sustentabilidade: resumos expandidos... Sete Lagoas: ABMS, 2010. 1 CD-ROM. Biblioteca(s): Embrapa Milho e Sorgo. |
| |
16. | | TAVARES, W. de S.; PEREIRA, A. I. de A.; MIELKE, O. H. H.; SERRÃO, J. E.; ZANUNCIO, J. C. Mechanitis polymnia casabranca and Ithomia lichyi lichyi (Lepidoptera: Nymphalidae) damaging tree of Solanum granuloso-leprosum (Solanaceae). Cerne, Lavras, v. 20, n. 1, p. 165-171, jan./mar. 2014. Biblioteca(s): Embrapa Florestas. |
| |
17. | | BARROS, L. C. de; TAVARES, W. de S.; BARROS, I. de R.; RIBEIRO, P. E. de A. Integração das tecnologias sociais Barraginhas e Lago de Múltiplo Uso. In: SIMPÓSIO BRASILEIRO DE AGROPECUÁRIA SUSTENTÁVEL, 2., 2010, Viçosa, MG. Anais... Viçosa, MG: Os Editores, 2010. p. 215-219. Biblioteca(s): Embrapa Milho e Sorgo. |
| |
18. | | BARROS, L. C. de; TAVARES, W. de S.; BARROS, I. de R.; RIBEIRO, P. E. de A. Integração das tecnologias sociais Barraginhas e Lago de Múltiplo Uso. In: BALSADI, O. V.; CRUZ, M. C. da; VERNE, M. C.; PEREIRA, V. da F.; SICOLI, A. H. (Ed.). Transferência de tecnologia e construção do conhecimento. Brasília, DF: Embrapa, 2013. p. 243-248. Biblioteca(s): Embrapa Milho e Sorgo. |
| |
20. | | TAVARES, W. de S.; ZANUNCIO, J. C.; SILVA, R. B. da; PETACCI, F.; CRUZ, I.; SERRÃO, J. E. Selectivity of botanical extracts of the cerrado on Tenebrio molitor (Coleoptera: tenebrionidae) and Anticarsia gemmatalis (Lepidoptera: noctuidae). In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. 1 CD-ROM. Biblioteca(s): Embrapa Milho e Sorgo. |
| |
Registros recuperados : 44 | |
|
|
Registro Completo
Biblioteca(s): |
Embrapa Solos. |
Data corrente: |
16/11/2018 |
Data da última atualização: |
11/11/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
ALESSI, A. M.; BIRD, S. M.; OATES, N. C.; LI, Y.; DOWLE, A. A.; NOVOTNY, E. H.; AZEVEDO, E. R. de; BENNETT, J. P.; POLIKARPOV, I.; YOUNG, J. P. W.; MCQUEEN-MASON, S. J.; BRUCE, N. C. |
Afiliação: |
ANNA M. ALESSI, UNIVERSITY OF YORK; SUSANNAH M. BIRD, UNIVERSITY OF YORK; NICOLA C. OATES, UNIVERSITY OF YORK; YI LI, UNIVERSITY OF YORK; ADAM A. DOWLE, UNIVERSITY OF YORK; ETELVINO HENRIQUE NOVOTNY, CNPS; EDUARDO R. DE AZEVEDO, USP; JOSEPH P. BENNETT, UNIVERSITY OF YORK; IGOR POLIKARPOV, USP; J. PETER W. YOUNG, UNIVERSITY OF YORK; SIMON J. MCQUEEN-MASON, UNIVERSITY OF YORK; NEIL C. BRUCE, UNIVERSITY OF YORK. |
Título: |
Defining functional diversity for lignocellulose degradation in a microbial community using multi-omics studies. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Biotechnology for Biofuels, v. 11, article 166, 2018. |
DOI: |
https://doi.org/10.1186/s13068-018-1164-2 |
Idioma: |
Inglês |
Conteúdo: |
Background: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacaulis, Leadbetterella and Truepera. The eukaryotic members of the community were enriched in peritrich ciliates from genus Telotrochidium that thrived in the liquid cultures compared to fungal species that were present in low abundance. A targeted metasecretome approach combined with metatranscriptomics analysis, identified 1127 proteins and showed the presence of numerous carbohydrate-active enzymes extracted from the biomassbound fractions and from the culture supernatant. This revealed a wide array of hydrolytic cellulases, hemicellulases and carbohydrate-binding modules involved in lignocellulose degradation. The expression of these activities correlated to the changes in the biomass composition observed by FTIR and ssNMR measurements. Conclusions: A combination of mass spectrometry-based proteomics coupled with metatranscriptomics has enabled the identification of a large number of lignocellulose degrading enzymes that can now be further explored for the development of improved enzyme cocktails for the treatment of plant-based feedstocks. In addition to the expected carbohydrate-active enzymes, our studies reveal a large number of unknown proteins, some of which may play a crucial role in community-based lignocellulose degradation. MenosBackground: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacau... Mostrar Tudo |
Palavras-Chave: |
CAZy; Metasecretome. |
Thesaurus NAL: |
Lignocellulose. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/186133/1/2018-044.pdf
|
Marc: |
LEADER 03644naa a2200301 a 4500 001 2099501 005 2021-11-11 008 2018 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1186/s13068-018-1164-2$2DOI 100 1 $aALESSI, A. M. 245 $aDefining functional diversity for lignocellulose degradation in a microbial community using multi-omics studies.$h[electronic resource] 260 $c2018 520 $aBackground: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacaulis, Leadbetterella and Truepera. The eukaryotic members of the community were enriched in peritrich ciliates from genus Telotrochidium that thrived in the liquid cultures compared to fungal species that were present in low abundance. A targeted metasecretome approach combined with metatranscriptomics analysis, identified 1127 proteins and showed the presence of numerous carbohydrate-active enzymes extracted from the biomassbound fractions and from the culture supernatant. This revealed a wide array of hydrolytic cellulases, hemicellulases and carbohydrate-binding modules involved in lignocellulose degradation. The expression of these activities correlated to the changes in the biomass composition observed by FTIR and ssNMR measurements. Conclusions: A combination of mass spectrometry-based proteomics coupled with metatranscriptomics has enabled the identification of a large number of lignocellulose degrading enzymes that can now be further explored for the development of improved enzyme cocktails for the treatment of plant-based feedstocks. In addition to the expected carbohydrate-active enzymes, our studies reveal a large number of unknown proteins, some of which may play a crucial role in community-based lignocellulose degradation. 650 $aLignocellulose 653 $aCAZy 653 $aMetasecretome 700 1 $aBIRD, S. M. 700 1 $aOATES, N. C. 700 1 $aLI, Y. 700 1 $aDOWLE, A. A. 700 1 $aNOVOTNY, E. H. 700 1 $aAZEVEDO, E. R. de 700 1 $aBENNETT, J. P. 700 1 $aPOLIKARPOV, I. 700 1 $aYOUNG, J. P. W. 700 1 $aMCQUEEN-MASON, S. J. 700 1 $aBRUCE, N. C. 773 $tBiotechnology for Biofuels$gv. 11, article 166, 2018.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Solos (CNPS) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|