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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
18/04/2000 |
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Data da última atualização: |
18/04/2000 |
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Autoria: |
ALVES, B. J. R.; REZENDE, C. de P.; RESENDE, A. S.; MACEDO, R.; TARRE, R. M.; URQUIAGA, S.; BODDEY, R. M. |
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Título: |
Estimation of N2 fixation in Desmodium ovalifolium from the relative ureide abundance of stem solutes: Comparison with the 15N-dilution and an in situ soil core technique. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Nutrient Cycling in Agroecosystems, v. 56, p. 177-193, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
Many, but not all, legumes of tropical origin, transport fixed N from the nodules to the shoot tissue in the form of ureides, and the mineral N absorbed from the soil is principally transported in the form of nitrate. The analysis of stem xylem sap, or hot-water extracts of stem tissue, for ureide and nitrate has been used successfully to quantify BNF contributions to several grain legumes and more recently to some shrub and forage legumes. The objective of this study was to investigate the application of this technique to the quantification of the contribution of BNF to the forage legume Desmodium ovalifolium by comparing the relative ureide abundance (RUA) of stem extracts of this plant with simultaneous estimates of BNF obtained using the 15N isotope dilution technique. The first experiment was performed in pots of soil, taken from a grazing study, amended with 15N-labelled organic matter at four different application rates. The ureide concentration in the stem extracts reflected the changes in BNF activity during plant growth and the RUA was closely correlated with the proportion of N derived from BNF as determined from the 15N technique (r2 = 0.86 and 0.88 for inoculated and non-inoculated and non-inoculated plants, respectively). The use of a calibration curve derived from a previous study where the same legume was fed increasing concentrations of 15N labelled nitrate in sand/vermiculite culture, resulted in an over-estimation of the BNF contribution which may have been due to a significant uptake of ammonium from this acidic soil. The second experiment was performed in field plots and a good agreement was found between the estimates of BNF derived from using the ureide and 15N dilution techniques at two harvests six months apart. The uptake of soil N by the D. ovalifolium and two forage grasses (Brachiaria humidicola and Panicum maximum) was estimated using an in situ soil core technique, and, while the uptake of N by the grasses was successfully estimated, this technique underestimated the N derived from the soil by the legume as determined by the ureide and 15N dilution techniques. MenosMany, but not all, legumes of tropical origin, transport fixed N from the nodules to the shoot tissue in the form of ureides, and the mineral N absorbed from the soil is principally transported in the form of nitrate. The analysis of stem xylem sap, or hot-water extracts of stem tissue, for ureide and nitrate has been used successfully to quantify BNF contributions to several grain legumes and more recently to some shrub and forage legumes. The objective of this study was to investigate the application of this technique to the quantification of the contribution of BNF to the forage legume Desmodium ovalifolium by comparing the relative ureide abundance (RUA) of stem extracts of this plant with simultaneous estimates of BNF obtained using the 15N isotope dilution technique. The first experiment was performed in pots of soil, taken from a grazing study, amended with 15N-labelled organic matter at four different application rates. The ureide concentration in the stem extracts reflected the changes in BNF activity during plant growth and the RUA was closely correlated with the proportion of N derived from BNF as determined from the 15N technique (r2 = 0.86 and 0.88 for inoculated and non-inoculated and non-inoculated plants, respectively). The use of a calibration curve derived from a previous study where the same legume was fed increasing concentrations of 15N labelled nitrate in sand/vermiculite culture, resulted in an over-estimation of the BNF contribution which may have bee... Mostrar Tudo |
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Palavras-Chave: |
BNF; FBN; Fixação biologica de nitrogênio; N; Nitrogen fixing bacteria. |
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Thesagro: |
Diluição Isotópica; Mineralização; Nitrogênio; Solo. |
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Thesaurus Nal: |
mineralization; nitrogen; soil; tracer techniques. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03144naa a2200349 a 4500
001 1621144
005 2000-04-18
008 2000 bl --- 0-- u #d
100 1 $aALVES, B. J. R.
245 $aEstimation of N2 fixation in Desmodium ovalifolium from the relative ureide abundance of stem solutes$bComparison with the 15N-dilution and an in situ soil core technique.
260 $c2000
520 $aMany, but not all, legumes of tropical origin, transport fixed N from the nodules to the shoot tissue in the form of ureides, and the mineral N absorbed from the soil is principally transported in the form of nitrate. The analysis of stem xylem sap, or hot-water extracts of stem tissue, for ureide and nitrate has been used successfully to quantify BNF contributions to several grain legumes and more recently to some shrub and forage legumes. The objective of this study was to investigate the application of this technique to the quantification of the contribution of BNF to the forage legume Desmodium ovalifolium by comparing the relative ureide abundance (RUA) of stem extracts of this plant with simultaneous estimates of BNF obtained using the 15N isotope dilution technique. The first experiment was performed in pots of soil, taken from a grazing study, amended with 15N-labelled organic matter at four different application rates. The ureide concentration in the stem extracts reflected the changes in BNF activity during plant growth and the RUA was closely correlated with the proportion of N derived from BNF as determined from the 15N technique (r2 = 0.86 and 0.88 for inoculated and non-inoculated and non-inoculated plants, respectively). The use of a calibration curve derived from a previous study where the same legume was fed increasing concentrations of 15N labelled nitrate in sand/vermiculite culture, resulted in an over-estimation of the BNF contribution which may have been due to a significant uptake of ammonium from this acidic soil. The second experiment was performed in field plots and a good agreement was found between the estimates of BNF derived from using the ureide and 15N dilution techniques at two harvests six months apart. The uptake of soil N by the D. ovalifolium and two forage grasses (Brachiaria humidicola and Panicum maximum) was estimated using an in situ soil core technique, and, while the uptake of N by the grasses was successfully estimated, this technique underestimated the N derived from the soil by the legume as determined by the ureide and 15N dilution techniques.
650 $amineralization
650 $anitrogen
650 $asoil
650 $atracer techniques
650 $aDiluição Isotópica
650 $aMineralização
650 $aNitrogênio
650 $aSolo
653 $aBNF
653 $aFBN
653 $aFixação biologica de nitrogênio
653 $aN
653 $aNitrogen fixing bacteria
700 1 $aREZENDE, C. de P.
700 1 $aRESENDE, A. S.
700 1 $aMACEDO, R.
700 1 $aTARRE, R. M.
700 1 $aURQUIAGA, S.
700 1 $aBODDEY, R. M.
773 $tNutrient Cycling in Agroecosystems$gv. 56, p. 177-193, 2000.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
09/11/1995 |
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Data da última atualização: |
30/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GODOY, R.; ABRAHÃO, J. T. M. |
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Afiliação: |
RODOLFO GODOY, CPPSE; JAIRO T. M. ABRAHÃO, USP-ESALQ. |
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Título: |
Testes de vigor em sementes de algodão (Gossypium hirsutum L.) deslintadas quimicamente. |
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Ano de publicação: |
1977 |
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Fonte/Imprenta: |
Anais ESALQ, v. 34, p. 247-265, 1977. |
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Idioma: |
Português |
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Conteúdo: |
Sementes deslintadas quimicamente de duas cultivares de algodão, armazenadas em camara seca e ambiente de laboratório, sem controle de temperatura a umidade de laboratório, sem controle de temperatura e umidade, foram submetidas a diversos testes de vigor e a um experimento de campo, com a finalidade de se procurar determinar os testes mais indicados para esse tipo de sementes. |
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Palavras-Chave: |
Gossypium hirsutum L; Sementes. |
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Thesagro: |
Algodão; Germinação; Teste de Vigor. |
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Thesaurus NAL: |
germination; seeds. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/42453/1/TesteVigorSementes.pdf
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Marc: |
LEADER 00984naa a2200217 a 4500
001 1042453
005 2022-11-30
008 1977 bl uuuu u00u1 u #d
100 1 $aGODOY, R.
245 $aTestes de vigor em sementes de algodão (Gossypium hirsutum L.) deslintadas quimicamente.$h[electronic resource]
260 $c1977
520 $aSementes deslintadas quimicamente de duas cultivares de algodão, armazenadas em camara seca e ambiente de laboratório, sem controle de temperatura a umidade de laboratório, sem controle de temperatura e umidade, foram submetidas a diversos testes de vigor e a um experimento de campo, com a finalidade de se procurar determinar os testes mais indicados para esse tipo de sementes.
650 $agermination
650 $aseeds
650 $aAlgodão
650 $aGerminação
650 $aTeste de Vigor
653 $aGossypium hirsutum L
653 $aSementes
700 1 $aABRAHÃO, J. T. M.
773 $tAnais ESALQ$gv. 34, p. 247-265, 1977.
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Embrapa Pecuária Sudeste (CPPSE) |
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