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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Norte / UEP-Parnaíba. |
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Data corrente: |
08/02/1994 |
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Data da última atualização: |
08/02/1994 |
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Autoria: |
BERNATZKY, R.; TANKSLEY, S. D. |
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Título: |
Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences |
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Ano de publicação: |
1986 |
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Fonte/Imprenta: |
Genetics Society of America, v.112, p.887-898, apr. 1986. |
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Idioma: |
Inglês |
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Conteúdo: |
A linkage map in tomato has beeen developed based on isozyme and random cDNA clones derived from mRNA. Interspecific backcross and F2 populations of Lycopersicon esculentum and L. pennellii were employed in the linkage analysis. Allelic differences in cDNA markers were based on restriction fragment length polymorphisms detected through analysis. A total of 57 unique cDNA clones have been analyzed. The majority of cDNA markers correspond to single loci and are dispersed throughout the genome. Of those clones that hybridize to two or more loci, most show genetic independence (ie., they are unlinked). The combination of isozyme, cDNA and previously mapped DNA markers total112 loci. It is estimated that aproximately 92% of the genome can be monitoredduring segregation with these markers. Molecular maps, such as the one being construced in tomato, may allow genetic and breeding experiments that previously were not possible. |
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Palavras-Chave: |
Marcadores moleculares; Melhoramento genetico. |
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Thesagro: |
Tomate. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01435naa a2200169 a 4500
001 1074146
005 1994-02-08
008 1986 bl --- 0-- u #d
100 1 $aBERNATZKY, R.
245 $aToward a saturated linkage map in tomato based on isozymes and random cDNA sequences
260 $c1986
520 $aA linkage map in tomato has beeen developed based on isozyme and random cDNA clones derived from mRNA. Interspecific backcross and F2 populations of Lycopersicon esculentum and L. pennellii were employed in the linkage analysis. Allelic differences in cDNA markers were based on restriction fragment length polymorphisms detected through analysis. A total of 57 unique cDNA clones have been analyzed. The majority of cDNA markers correspond to single loci and are dispersed throughout the genome. Of those clones that hybridize to two or more loci, most show genetic independence (ie., they are unlinked). The combination of isozyme, cDNA and previously mapped DNA markers total112 loci. It is estimated that aproximately 92% of the genome can be monitoredduring segregation with these markers. Molecular maps, such as the one being construced in tomato, may allow genetic and breeding experiments that previously were not possible.
650 $aTomate
653 $aMarcadores moleculares
653 $aMelhoramento genetico
700 1 $aTANKSLEY, S. D.
773 $tGenetics Society of America$gv.112, p.887-898, apr. 1986.
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Registro original: |
Embrapa Meio Norte / UEP-Parnaíba (CPAMN-UEPP) |
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