|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
19/10/2020 |
Data da última atualização: |
19/10/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SANTOS, V. M. B. dos; PINTO, P. H. N.; BALARO, M. F. A.; SANTOS, J. D. R.; TAIRA, A. R.; ESPIRITO SANTO, C. G. do; GONÇALVES, F. M.; FONSECA, J. F. da; BRANDÃO, F. Z. |
Afiliação: |
VANESSA M. B. DOS SANTOS, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; PEDRO HENRIQUE N. PINTO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; MARIO FELIPE A. BALARO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JULIANA D. R. SANTOS, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; AUGUSTO R. TAIRA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; CAROLINE G. DO ESPIRITO SANTO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; FERNANDA M. GONÇALVES, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC; FELIPE Z. BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil. |
Título: |
Use of oxytocin to attain cervical dilation for transcervical embryo transfer in sheep. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Reproduction in Domestic Animals, v. 55, n. 10, p. 1446-1454, Oct. 2020. |
DOI: |
https://doi.org/10.1111/rda.13795 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The aim of this work was to determine whether a cervical dilation protocol (CDP) composed of only oxytocin can be used to perform transcervical (non?surgical) embryo transfer in sheep (NSET) without affecting the viability of the corpus luteum (CL). Likewise, we evaluated whether a cervical transposing test with a Hegar dilator (CT Hegar test), performed at oestrous time, could be used to screen ewes for NSET (greater or lower chances to transpose the cervix). For that, oestrous and ovulation synchronization was performed in 25 Santa Inês ewes to induce the dioestrous condition. Animals went through the following CDP in a crossover design: E + OX, oestradiol benzoate (100 µg intravenously [IV]) and oxytocin (100 IU IV); OX, oxytocin (100 IU IV); and SAL, saline solution (IV). Using a Hegar dilator, cervical transposing attempts were performed at oestrous (D0) and dioestrous time (D8). The viability of the CL (morphology, luteal blood flow and progesterone values) was evaluated by ultrasonography (colour Doppler and B?mode) and by serum progesterone measurement from D7 to D13. The cervical transposing rate was lower for the SAL group (64%; 16/25; p < .05) and did not differ between the E + OX (88%; 22/25, p > .05) and OX (84%; 21/25, p > .05) groups. No treatment affected the CL viability. The CT Hegar test showed a high sensitivity (85.7%?93.3%), satisfactory accuracy (72%?84%), low false?negative rate (6.7%?14.6%), but high false?positive rate (46%?66.7%). In conclusion, a CDP protocol composed exclusively of oxytocin can lead to good cervical transposing rates and does not affect the viability of the CL. In addition, a screening test (CT Hegar) performed at oestrus can identify ewes for which cervical transposing will likely not occur at NSET. MenosAbstract: The aim of this work was to determine whether a cervical dilation protocol (CDP) composed of only oxytocin can be used to perform transcervical (non?surgical) embryo transfer in sheep (NSET) without affecting the viability of the corpus luteum (CL). Likewise, we evaluated whether a cervical transposing test with a Hegar dilator (CT Hegar test), performed at oestrous time, could be used to screen ewes for NSET (greater or lower chances to transpose the cervix). For that, oestrous and ovulation synchronization was performed in 25 Santa Inês ewes to induce the dioestrous condition. Animals went through the following CDP in a crossover design: E + OX, oestradiol benzoate (100 µg intravenously [IV]) and oxytocin (100 IU IV); OX, oxytocin (100 IU IV); and SAL, saline solution (IV). Using a Hegar dilator, cervical transposing attempts were performed at oestrous (D0) and dioestrous time (D8). The viability of the CL (morphology, luteal blood flow and progesterone values) was evaluated by ultrasonography (colour Doppler and B?mode) and by serum progesterone measurement from D7 to D13. The cervical transposing rate was lower for the SAL group (64%; 16/25; p < .05) and did not differ between the E + OX (88%; 22/25, p > .05) and OX (84%; 21/25, p > .05) groups. No treatment affected the CL viability. The CT Hegar test showed a high sensitivity (85.7%?93.3%), satisfactory accuracy (72%?84%), low false?negative rate (6.7%?14.6%), but high false?positive rate (46%?66.7%). In conc... Mostrar Tudo |
Palavras-Chave: |
Dilatation; Luteal phase. |
Thesagro: |
Ovelha; Ovino; Reprodução Animal; Transferência de Embrião. |
Thesaurus Nal: |
Animal reproduction; Embryo transfer; Oxytocin; Sheep. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02824naa a2200349 a 4500 001 2125631 005 2020-10-19 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1111/rda.13795$2DOI 100 1 $aSANTOS, V. M. B. dos 245 $aUse of oxytocin to attain cervical dilation for transcervical embryo transfer in sheep.$h[electronic resource] 260 $c2020 520 $aAbstract: The aim of this work was to determine whether a cervical dilation protocol (CDP) composed of only oxytocin can be used to perform transcervical (non?surgical) embryo transfer in sheep (NSET) without affecting the viability of the corpus luteum (CL). Likewise, we evaluated whether a cervical transposing test with a Hegar dilator (CT Hegar test), performed at oestrous time, could be used to screen ewes for NSET (greater or lower chances to transpose the cervix). For that, oestrous and ovulation synchronization was performed in 25 Santa Inês ewes to induce the dioestrous condition. Animals went through the following CDP in a crossover design: E + OX, oestradiol benzoate (100 µg intravenously [IV]) and oxytocin (100 IU IV); OX, oxytocin (100 IU IV); and SAL, saline solution (IV). Using a Hegar dilator, cervical transposing attempts were performed at oestrous (D0) and dioestrous time (D8). The viability of the CL (morphology, luteal blood flow and progesterone values) was evaluated by ultrasonography (colour Doppler and B?mode) and by serum progesterone measurement from D7 to D13. The cervical transposing rate was lower for the SAL group (64%; 16/25; p < .05) and did not differ between the E + OX (88%; 22/25, p > .05) and OX (84%; 21/25, p > .05) groups. No treatment affected the CL viability. The CT Hegar test showed a high sensitivity (85.7%?93.3%), satisfactory accuracy (72%?84%), low false?negative rate (6.7%?14.6%), but high false?positive rate (46%?66.7%). In conclusion, a CDP protocol composed exclusively of oxytocin can lead to good cervical transposing rates and does not affect the viability of the CL. In addition, a screening test (CT Hegar) performed at oestrus can identify ewes for which cervical transposing will likely not occur at NSET. 650 $aAnimal reproduction 650 $aEmbryo transfer 650 $aOxytocin 650 $aSheep 650 $aOvelha 650 $aOvino 650 $aReprodução Animal 650 $aTransferência de Embrião 653 $aDilatation 653 $aLuteal phase 700 1 $aPINTO, P. H. N. 700 1 $aBALARO, M. F. A. 700 1 $aSANTOS, J. D. R. 700 1 $aTAIRA, A. R. 700 1 $aESPIRITO SANTO, C. G. do 700 1 $aGONÇALVES, F. M. 700 1 $aFONSECA, J. F. da 700 1 $aBRANDÃO, F. Z. 773 $tReproduction in Domestic Animals$gv. 55, n. 10, p. 1446-1454, Oct. 2020.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
07/04/1995 |
Data da última atualização: |
07/04/1995 |
Autoria: |
MAGALHAES, J. C. A. J. de. |
Título: |
Efeito de niveis de calcario e de fosforo em duas variedades de trigo. |
Ano de publicação: |
1979 |
Fonte/Imprenta: |
Revista Brasileira de Ciencia do Solo, v.3, n.1, p.24-29, 1979. |
Idioma: |
Português |
Palavras-Chave: |
Wheats. |
Thesagro: |
Calcário; Cerrado; Fósforo; Trigo; Triticum Aestivum. |
Thesaurus NAL: |
limestone; phosphorus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00559naa a2200205 a 4500 001 1549861 005 1995-04-07 008 1979 bl --- 0-- u #d 100 1 $aMAGALHAES, J. C. A. J. de 245 $aEfeito de niveis de calcario e de fosforo em duas variedades de trigo. 260 $c1979 650 $alimestone 650 $aphosphorus 650 $aCalcário 650 $aCerrado 650 $aFósforo 650 $aTrigo 650 $aTriticum Aestivum 653 $aWheats 773 $tRevista Brasileira de Ciencia do Solo$gv.3, n.1, p.24-29, 1979.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Cerrados (CPAC) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|