Data/hora: 22/06/2024 16:49:15

Biblioteca(s): 	Embrapa Caprinos e Ovinos.	
Data corrente: 	06/10/2008	
Data da última atualização: 	24/08/2023	
Autoria: 	SUTRA, L.; MENDOLIA, C.; RAINARD, P.; POUTREL, B.	
Título: 	Encapsulation of Staphylococcus aureus isolates from mastitic milk: relationship between Capsular Polysaccharide Types 5 and 8 and colony morphology in serum-soft agar, clumping factor, teichoic acid, and protein A.	
Ano de publicação: 	1990	
Fonte/Imprenta: 	Journal of Clinical Microbiology, v. 28, n. 3, p. 447-451, Mar. 1990.	
DOI: 	https://doi.org/10.1128/jcm.28.3.447-451.1990	
Idioma: 	Inglês	
Conteúdo: 	Abstract: A total of 193 Staphylococcus aureus isolates from bovine, caprine, and ovine mastitis producing type 5 or 8 capsular polysaccharides were investigated for colony morphology in serum-soft agar and agglutinability by an anti-teichoic acid serum, after cultivation in modified staphylococcus medium no. 110. Also, 40 of these strains were cultivated in brain heart infusion and submitted to clumping factor and protein A detection tests. Considering capsular serotyping as a reference method, diffuse growth in serum-soft agar and inagglutinability by anti-teichoic acid serum identified, respectively, 57.5 and 45% of encapsulated strains cultivated in brain heart infusion and 85.5 and 77.2% of those cultivated in modified staphylococcus medium 110. Consequently, these indirect techniques underestimated encapsulation and were greatly influenced by culture conditions. Whatever the medium used, diffuse colony morphology in serum-soft agar was generally characterized by a masking of teichoic acid and protein A. By contrast, these surface antigens were detected in association with compact morphology; the presence of a thin or discontinuous capsular material could explain this result. Moreover, the masking of teichoic acid and the removal of capsular polysaccharide by washing in saline suggest that type 8 capsular polysaccharide is more abundant and labile than type 5.	
Palavras-Chave: 	Mastite; Staphylococcal Protein A.	
Thesagro: 	Staphylococcus Aureus.	
Thesaurus Nal: 	Bovine mastitis; Cattle diseases; Goat diseases; Immunology; Infection; Microbiology; Milk; Polysaccharides; Sheep diseases; Teichoic acids.	
Categoria do assunto: 	H Saúde e Patologia	
Registro original: 	Embrapa Caprinos e Ovinos (CNPC)	
Exemplares: 	CNPC - (21411-1) - ADD - AP - DD -  -  -  - DIS