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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
21/03/2022 |
Data da última atualização: |
22/03/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GALLENTI, R.; HUSSEIN, H. E.; ALZAN, H. F.; SUAREZ, C. E.; UETI, M.; ASURMENDI, S.; BENITEZ, D.; ARAUJO, F. R.; ROLLS, P.; SIBEKO-MATJILA, K.; SCHNITTGER, L.; FLORIN-CHRISTENSEN, M. |
Afiliação: |
ROMINA GALLENTI, Instituto Nacional de Tecnología Agropecuaria-Consejo Nacional de Investigaciones Científicas y Técnicas (INTA-CONICET), Argentina.; HALA E. HUSSEIN, Washington State University, USA.; HEBA F. ALZAN, Washington State University, USA.; CARLOS E. SUAREZ, Washington State University, USA.; MASSARO UETI, Washington State University, USA.; SEBASTIÁN ASURMENDI, Instituto Nacional de Tecnología Agropecuaria-Consejo Nacional de Investigaciones Científicas y Técnicas (INTA-CONICET), Argentina.; DANIEL BENITEZ, Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina.; FLABIO RIBEIRO DE ARAUJO, CNPGC; PETER ROLLS, Department of Agriculture & Fisheries, Tick Fever Centre, Australia.; KGOMOTSO SIBEKO-MATJILA, University of Pretoria, South Africa.; LEONHARD SCHNITTGER, Instituto Nacional de Tecnología Agropecuaria-Consejo Nacional de Investigaciones Científicas y Técnicas (INTA-CONICET), Argentina.; MÓNICA FLORIN-CHRISTENSEN, Instituto Nacional de Tecnología Agropecuaria-Consejo Nacional de Investigaciones Científicas y Técnicas (INTA-CONICET), Argentina. |
Título: |
Unraveling the complexity of the Rhomboid Serine Protease 4 Family of Babesia bovis using bioinformatics and experimental studies. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Pathogens, v. 11, n. 344, 2022. |
Idioma: |
Inglês |
Conteúdo: |
Babesia bovis, a tick-transmitted apicomplexan protozoon, infects cattle in tropical and subtropical regions around the world. In the apicomplexans Toxoplasma gondii and Plasmodium falciparum, rhomboid serine protease 4 (ROM4) fulfills an essential role in host cell invasion. We thus investigated B. bovis ROM4 coding genes; their genomic organization; their expression in in vitro cultured asexual (AS) and sexual stages (SS); and strain polymorphisms. B. bovis contains five rom4 paralogous genes in chromosome 2, which we have named rom4.1, 4.2, 4.3, 4.4 and 4.5. There are moderate degrees of sequence identity between them, except for rom4.3 and 4.4, which are almost identical. RT-qPCR analysis showed that rom4.1 and rom4.3/4.4, respectively, display 18-fold and 218-fold significantly higher (p < 0.01) levels of transcription in SS than in AS, suggesting a role in gametogenesis-related processes. In contrast, transcription of rom4.4 and 4.5 differed non-significantly between the stages. ROM4 polymorphisms among geographic isolates were essentially restricted to the number of tandem repeats of a 29-amino acid sequence in ROM4.5. This sequence repeat is highly conserved and predicted as antigenic. B. bovis ROMs likely participate in relevant host?pathogen interactions and are possibly useful targets for the development of new control strategies against this pathogen. |
Palavras-Chave: |
Inter-strain polymorphism; Rhomboid serine proteases. |
Thesagro: |
Babesia Bovis. |
Thesaurus Nal: |
Babesiosis; Gene expression; Tick-borne diseases. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/232800/1/2022-Babesia-ROM4.pdf
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Marc: |
LEADER 02337naa a2200325 a 4500 001 2141112 005 2022-03-22 008 2022 bl uuuu u00u1 u #d 100 1 $aGALLENTI, R. 245 $aUnraveling the complexity of the Rhomboid Serine Protease 4 Family of Babesia bovis using bioinformatics and experimental studies.$h[electronic resource] 260 $c2022 520 $aBabesia bovis, a tick-transmitted apicomplexan protozoon, infects cattle in tropical and subtropical regions around the world. In the apicomplexans Toxoplasma gondii and Plasmodium falciparum, rhomboid serine protease 4 (ROM4) fulfills an essential role in host cell invasion. We thus investigated B. bovis ROM4 coding genes; their genomic organization; their expression in in vitro cultured asexual (AS) and sexual stages (SS); and strain polymorphisms. B. bovis contains five rom4 paralogous genes in chromosome 2, which we have named rom4.1, 4.2, 4.3, 4.4 and 4.5. There are moderate degrees of sequence identity between them, except for rom4.3 and 4.4, which are almost identical. RT-qPCR analysis showed that rom4.1 and rom4.3/4.4, respectively, display 18-fold and 218-fold significantly higher (p < 0.01) levels of transcription in SS than in AS, suggesting a role in gametogenesis-related processes. In contrast, transcription of rom4.4 and 4.5 differed non-significantly between the stages. ROM4 polymorphisms among geographic isolates were essentially restricted to the number of tandem repeats of a 29-amino acid sequence in ROM4.5. This sequence repeat is highly conserved and predicted as antigenic. B. bovis ROMs likely participate in relevant host?pathogen interactions and are possibly useful targets for the development of new control strategies against this pathogen. 650 $aBabesiosis 650 $aGene expression 650 $aTick-borne diseases 650 $aBabesia Bovis 653 $aInter-strain polymorphism 653 $aRhomboid serine proteases 700 1 $aHUSSEIN, H. E. 700 1 $aALZAN, H. F. 700 1 $aSUAREZ, C. E. 700 1 $aUETI, M. 700 1 $aASURMENDI, S. 700 1 $aBENITEZ, D. 700 1 $aARAUJO, F. R. 700 1 $aROLLS, P. 700 1 $aSIBEKO-MATJILA, K. 700 1 $aSCHNITTGER, L. 700 1 $aFLORIN-CHRISTENSEN, M. 773 $tPathogens$gv. 11, n. 344, 2022.
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Embrapa Gado de Corte (CNPGC) |
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Registros recuperados : 4 | |
1. | | MADRUGA, C. R.; SUAREZ, C. E.; MCELWAIN, T. F. Identificacao de antigenos de merozoitos de isolado mexicano de Babesia bovis e Babesia bigemina por soro imune de isolados do Brasil. In:SEMINARIO DO COLEGIO BRASILEIRO DE PARASITOLOGIA VETERINARIA, 7.; SIMPOSIO SOBRE A MOSCA-DOS-CHIFRES, 2., 1991, Sao Paulo. Revista Brasileira de Parasitologia Veterinaria, Sao Paulo, v.1, n.0, p.29, 1991. Resumo 1-21. CNPGC.Biblioteca(s): Embrapa Gado de Corte. |
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2. | | MADRUGA, C. R.; SUAREZ, C. E.; KESSLER, R. H.; SCHENK, M. A. M.; MIGUITA, M. Analise antigenica da Babesia bigemina : comparacao entre isolados brasileiro; argentino e mexicano selvagens com cepa vacional utilizada no Brasil. In: SEMINARIO BRASILEIRO DE PRASITOLOGIA VETERINARIA, 9., 1995, Campo Grande. Anais. Revista Brasileira de Parasitologia Veterinaria, Sao Paulo, v.4, n.2, p.189, ago., 1995. Suplemento, 1. CNPGC. Resumo.Biblioteca(s): Embrapa Gado de Corte. |
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3. | | MADRUGA, C. R.; SUAREZ, C. E.; SCHENK, M. A. M.; KESSLER, R. H.; MIGUITA, M. Analise antigenica dos isolados virulentos, atenuado de Anaplasma maginale e A. centrale. In: SEMINARIO BRASILEIRO DE PRASITOLOGIA VETERINARIA, 9., 1995, Campo Grande. Anais. Revista Brasileira de Parasitologia Veterinaria, Sao Paulo, v.4, n.2, p.190, ago., 1995. Suplemento, 1. CNPGC. Resumo.Biblioteca(s): Embrapa Gado de Corte. |
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4. | | GALLENTI, R.; HUSSEIN, H. E.; ALZAN, H. F.; SUAREZ, C. E.; UETI, M.; ASURMENDI, S.; BENITEZ, D.; ARAUJO, F. R.; ROLLS, P.; SIBEKO-MATJILA, K.; SCHNITTGER, L.; FLORIN-CHRISTENSEN, M. Unraveling the complexity of the Rhomboid Serine Protease 4 Family of Babesia bovis using bioinformatics and experimental studies. Pathogens, v. 11, n. 344, 2022.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
Biblioteca(s): Embrapa Gado de Corte. |
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Registros recuperados : 4 | |
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