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| Registros recuperados : 186 | |
| 31. |  | STURION, J. A.; IEDE, E. T. Influência da profundidade de semeadura, cobertura do canteiro e sombreamento na formação de mudas de Ocotea porosa (NEES) Liberato Barroso (Imbuia). Silvicultura, São Paulo, v. 8, n. 28, p. 513-516, 1983. Edição dos anais do 4º Congresso Florestal Brasileiro, 1982, Belo Horizonte.| Biblioteca(s): Embrapa Florestas. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
05/06/2020 |
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Data da última atualização: |
16/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SOARES, A. C.; SOARES, J. C.; PASCHOALIN, R. T.; RODRIGUES, V. C.; MELENDEZ, M. E.; REIS, R. M.; CARVALHO, A. L.; MATTOSO, L. H. C.; OLIVEIRA, O. N. |
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Afiliação: |
LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA. |
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Título: |
Immunosensors containing solution blow spun fibers of poly(lactic acid) to detect p53 biomarker. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Materials Science & Engineering C, v. 115, 2020. |
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Páginas: |
1-10 |
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ISSN: |
0928-4931 |
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DOI: |
https://doi.org/10.1016/j.msec.2020.111120 |
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Idioma: |
Inglês |
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Conteúdo: |
This paper reports on biosensors made with a matrix of polylactic acid (PLA) fibers, which are suitable for immobilization of the anti-p53 active layer for detection of p53 biomarker. The PLA fibers were produced with solution blow spinning, a method that is advantageous for its simplicity and possibility to tune the fiber properties. For the biosensors, the optimized time to deposit the fibers was 60 s, with which detection of p53 could be achieved with the limit of detection of 11 pg/mL using electrical impedance spectroscopy. This sensitivity is also sufficient to detect the p53 biomarker in patient samples, which was confirmed by distinguishing samples from cell lines with distinct p53 concentrations in a plot where the impedance spectra were visualized with the interactive document mapping (IDMAP) technique. The high sensitivity and selectivity of the biosensors may be attributed to the specific interaction between the active layer and p53 modeled with a Langmuir-Freundlich and Freundlich isotherms and inferred from the analysis of the vibrational bands at 1550, 1650 and 1757 cm−1 using polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). The successful immobilization of the active layer is evidence that the approach based on solution blown spun fibers may be replicated to other types of biosensors. |
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Palavras-Chave: |
Immunosensor; PLA fibers; Solution blow spinning. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02209naa a2200289 a 4500
001 2123069
005 2022-08-16
008 2020 bl uuuu u00u1 u #d
022 $a0928-4931
024 7 $ahttps://doi.org/10.1016/j.msec.2020.111120$2DOI
100 1 $aSOARES, A. C.
245 $aImmunosensors containing solution blow spun fibers of poly(lactic acid) to detect p53 biomarker.$h[electronic resource]
260 $c2020
300 $a1-10
520 $aThis paper reports on biosensors made with a matrix of polylactic acid (PLA) fibers, which are suitable for immobilization of the anti-p53 active layer for detection of p53 biomarker. The PLA fibers were produced with solution blow spinning, a method that is advantageous for its simplicity and possibility to tune the fiber properties. For the biosensors, the optimized time to deposit the fibers was 60 s, with which detection of p53 could be achieved with the limit of detection of 11 pg/mL using electrical impedance spectroscopy. This sensitivity is also sufficient to detect the p53 biomarker in patient samples, which was confirmed by distinguishing samples from cell lines with distinct p53 concentrations in a plot where the impedance spectra were visualized with the interactive document mapping (IDMAP) technique. The high sensitivity and selectivity of the biosensors may be attributed to the specific interaction between the active layer and p53 modeled with a Langmuir-Freundlich and Freundlich isotherms and inferred from the analysis of the vibrational bands at 1550, 1650 and 1757 cm−1 using polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). The successful immobilization of the active layer is evidence that the approach based on solution blown spun fibers may be replicated to other types of biosensors.
653 $aImmunosensor
653 $aPLA fibers
653 $aSolution blow spinning
700 1 $aSOARES, J. C.
700 1 $aPASCHOALIN, R. T.
700 1 $aRODRIGUES, V. C.
700 1 $aMELENDEZ, M. E.
700 1 $aREIS, R. M.
700 1 $aCARVALHO, A. L.
700 1 $aMATTOSO, L. H. C.
700 1 $aOLIVEIRA, O. N.
773 $tMaterials Science & Engineering C$gv. 115, 2020.
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