Data/hora: 20/06/2024 16:08:04

Biblioteca(s): | Embrapa Florestas.
Data corrente: | 24/03/2015
Data da última atualização: | 24/03/2015
Autoria: | HERINGER, A. S.; STEINMACHER, D. A.; FRAGA, H. P. F.; VIEIRA, L. N.; REE, J. F.; GUERRA, M. P.
Título: | Global DNA methylation profiles of somatic embryos of peach palm (Bactris gasipaes Kunth) are influenced by cryoprotectants and droplet-vitrification cryopreservation.
Ano de publicação: | 2013
Fonte/Imprenta: | Plant Cell Tissue and Organ Culture, v. 114, p. 365-372, 2013.
Idioma: | Inglês
Conteúdo: | Regrowth capacity and genetic stability of plants recovered following cryopreservation are associated with changes in DNA epigenetics, particularly in DNA methylation levels. In this study, global DNA methylation profiles associated with frequency of regrowth of peach palm ( Bactris gasipaes ) somatic embryos following cryo- preservation using droplet-vitrification were investigated. Somatic embryo clusters (SEC) subjected to plant vitrifi- cation solution 3 (PVS3) for different durations (0, 60, 120, 180, and 240 min) were evaluated for regrowth capacity. The highest frequency of regrowth (52.4 %) was obtained when SEC were incubated in PVS3 for 120 min prior to droplet-vitrification cryopreservation. Global DNA meth- ylation profiles were influenced by both cryoprotectants and droplet-vitrification cryopreservation. Incubation of SEC in PVS3 for limited durations not only reduced fre- quency of regrowth, but also increased DNA methylations levels when compared with proliferating SEC grown in a temporary immersion system. Although SEC subjected to cryopreservation exhibited the highest DNA methylation variation, 120 min SEC incubation in a PVS3 solution resulted in the recovery of initial global methylation pro- files after 24 weeks of regrowth.
Palavras-Chave: | Espécie florestal; Pupunheira.
Thesagro: | Bactris Gasipaes; Criopreservação; Embriogénese; Espécie Nativa; Palmito; Pupunha.
Categoria do assunto: | --
Registro original: | Embrapa Florestas (CNPF)
Exemplares: | CNPF - (53555-1) - ADD - AP - DD -  -  -  - DIS