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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
10/06/2022 |
Data da última atualização: |
11/07/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ZANELLA, L. B.; DEGENHARDT, J.; STEINER, N.; TOMASI, J.; KESTRING, D. R.; QUOIRIN, M. |
Afiliação: |
LAUDIANE BRUNA ZANELLA, Federal University of Parana; JULIANA DEGENHARDT GOLDBACH, CNPF; NEUSA STEINER, Federal University of Santa Catarina; JÉSSICA TOMASI, Federal University of Parana; DAIANE RIGONI, CNPF; MARGUERITE QUOIRIN, Federal University of Parana. |
Título: |
Initiation of somatic embryogenesis in Pinus caribaea var. hondurensis using mature female gametophytes as explants. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
South African Journal of Botany, v. 149, p. 124-133, 2022. |
DOI: |
https://doi.org/10.1016/j.sajb.2022.05.053 |
Idioma: |
Português |
Conteúdo: |
ABSTRACT: Due to the difficulties of harvesting immature cones in genetic breeding orchards, such as high costs and the considerable time required, this study aimed to evaluate the somatic embryogenesis (SE) initiation of Pinus caribaea var. hondurensis using the mature female gametophyte as the initial explant. The basic medium for embryogenesis initiation was composed of QL (Quoirin and Lepoivre, 1977) nutrients, sucrose, myo-inositol, glutamine, casein hydrolisate, thiamine, 2,4-D, BAP and agar. This medium was compared with DCR (Gupta and Durzan, 1985) and WV5 (Coke, 1996) media, supplemented or not with 0.1 g L?1 folic acid (FA). Afterwards, three concentrations of 2,4-D (10, 20 and 30 ?M) combined or not with BAP (2, 4 and 8 ?M) were tested. After 90 days the percentages of explants with embryogenic tissue (ET), embryo-like structures and oxidation were evaluated. We observed somatic tissue initiation` on mature female gametophytes. On QL medium supplemented with FA, the initiation was observed on up to 42% of female gametophytes. The combination of 10 ?M 2,4-D and 4 ?M BAP resulted in the highest initiation rate. The formation of ET was confirmed in all treatments by a histochemical test. Embryo-like structures developed on QL medium supplemented with FA, were first transferred to basic medium supplemented with 1.5 g L?1 AC for 30 days and finally to the same medium supplemented with 10% polyethylene glycol 3350 and 120 µM abscisic acid. The expression of SERK-1 and GER-1 genes was analyzed. SERK-1 was expressed only in the ET, while GER-1 was expressed in both the embryogenic and non-embryogenic tissues (non-ET). However, the somatic embryos studied through morphological and histochemical tests proved to be abnormal and did not develop further. MenosABSTRACT: Due to the difficulties of harvesting immature cones in genetic breeding orchards, such as high costs and the considerable time required, this study aimed to evaluate the somatic embryogenesis (SE) initiation of Pinus caribaea var. hondurensis using the mature female gametophyte as the initial explant. The basic medium for embryogenesis initiation was composed of QL (Quoirin and Lepoivre, 1977) nutrients, sucrose, myo-inositol, glutamine, casein hydrolisate, thiamine, 2,4-D, BAP and agar. This medium was compared with DCR (Gupta and Durzan, 1985) and WV5 (Coke, 1996) media, supplemented or not with 0.1 g L?1 folic acid (FA). Afterwards, three concentrations of 2,4-D (10, 20 and 30 ?M) combined or not with BAP (2, 4 and 8 ?M) were tested. After 90 days the percentages of explants with embryogenic tissue (ET), embryo-like structures and oxidation were evaluated. We observed somatic tissue initiation` on mature female gametophytes. On QL medium supplemented with FA, the initiation was observed on up to 42% of female gametophytes. The combination of 10 ?M 2,4-D and 4 ?M BAP resulted in the highest initiation rate. The formation of ET was confirmed in all treatments by a histochemical test. Embryo-like structures developed on QL medium supplemented with FA, were first transferred to basic medium supplemented with 1.5 g L?1 AC for 30 days and finally to the same medium supplemented with 10% polyethylene glycol 3350 and 120 µM abscisic acid. The expression of SERK-1 and G... Mostrar Tudo |
Palavras-Chave: |
Embryogenic tissues; GER-1; QL medium; SERK-1; Tecidos embriogênicos. |
Thesagro: |
Ácido Fólico. |
Thesaurus Nal: |
Folic acid; Pinus caribaea var. hondurensis. |
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
Marc: |
LEADER 02667naa a2200289 a 4500 001 2143947 005 2022-07-11 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.sajb.2022.05.053$2DOI 100 1 $aZANELLA, L. B. 245 $aInitiation of somatic embryogenesis in Pinus caribaea var. hondurensis using mature female gametophytes as explants.$h[electronic resource] 260 $c2022 520 $aABSTRACT: Due to the difficulties of harvesting immature cones in genetic breeding orchards, such as high costs and the considerable time required, this study aimed to evaluate the somatic embryogenesis (SE) initiation of Pinus caribaea var. hondurensis using the mature female gametophyte as the initial explant. The basic medium for embryogenesis initiation was composed of QL (Quoirin and Lepoivre, 1977) nutrients, sucrose, myo-inositol, glutamine, casein hydrolisate, thiamine, 2,4-D, BAP and agar. This medium was compared with DCR (Gupta and Durzan, 1985) and WV5 (Coke, 1996) media, supplemented or not with 0.1 g L?1 folic acid (FA). Afterwards, three concentrations of 2,4-D (10, 20 and 30 ?M) combined or not with BAP (2, 4 and 8 ?M) were tested. After 90 days the percentages of explants with embryogenic tissue (ET), embryo-like structures and oxidation were evaluated. We observed somatic tissue initiation` on mature female gametophytes. On QL medium supplemented with FA, the initiation was observed on up to 42% of female gametophytes. The combination of 10 ?M 2,4-D and 4 ?M BAP resulted in the highest initiation rate. The formation of ET was confirmed in all treatments by a histochemical test. Embryo-like structures developed on QL medium supplemented with FA, were first transferred to basic medium supplemented with 1.5 g L?1 AC for 30 days and finally to the same medium supplemented with 10% polyethylene glycol 3350 and 120 µM abscisic acid. The expression of SERK-1 and GER-1 genes was analyzed. SERK-1 was expressed only in the ET, while GER-1 was expressed in both the embryogenic and non-embryogenic tissues (non-ET). However, the somatic embryos studied through morphological and histochemical tests proved to be abnormal and did not develop further. 650 $aFolic acid 650 $aPinus caribaea var. hondurensis 650 $aÁcido Fólico 653 $aEmbryogenic tissues 653 $aGER-1 653 $aQL medium 653 $aSERK-1 653 $aTecidos embriogênicos 700 1 $aDEGENHARDT, J. 700 1 $aSTEINER, N. 700 1 $aTOMASI, J. 700 1 $aKESTRING, D. R. 700 1 $aQUOIRIN, M. 773 $tSouth African Journal of Botany$gv. 149, p. 124-133, 2022.
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Embrapa Florestas (CNPF) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
19/12/2018 |
Data da última atualização: |
19/12/2018 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SAMUEL-ROSA, A.; DALMOLIN, R. S. D.; GUBIANI, P. I.; OLIVEIRA, S. R. de M.; TEIXEIRA, W. G.; VIANA, J. H. M.; RIBEIRO, E.; TORNQUIST, C. G.; ANJOS, L. H. C. dos; SOUZA, J. J. E. L. de; OTTONI, M. V.; MEDEIROS, P. S. C. de; GRIS, D. J.; ROSIN, N. A.; BUENO, J. M. M.; SANTOS, H. G. dos; WEBER, E. J.; FLORES, C. A.; COSTA, E. M.; OLIVEIRA, R. P. de; FILIPPINI ALBA, J. M.; PEDROSO NETO, J. C.; PEDRON, F. de A.; CAVIGLIONE, J. H.; VALLADARES, G. S.; MIRANDA, C. S. S.; DEMATTÊ, J. A. M.; MARQUES JÚNIOR, J.; SIQUEIRA, D. S.; AQUINO, R. E. de; SILVERO, N. E. Q.; GENÚ, A. M.; BROETTO, T.; CANCIAN, L. C.; MIGUEL, P.; ZALAMENA, J.; DOTTO, A. C.; ALMEIDA, J. A. de; REICHERT.; CURCIO, G. R.; COLLIER, L. S.; CARVALHO JUNIOR, W. de; FONTANA, A.; OLIVEIRA, A. P. de; VOGELMANN, E. S.; MALLMANN, F. J. K.; VASQUES, G. de M.; LEPSCH, I. F.; FINK, J. R.; KER, J. C.; SILVA, L. S. da; FREITAS, P. L. de; BIELUCZYK, B.; TIECHER, T. |
Afiliação: |
ALESSANDRO SAMUEL-ROSA, UFSM; RICARDO SIMÃO DINIZ DALMOLIN, UFSM; PAULO IVONIR GUBIANI, UFSM; STANLEY ROBSON DE MEDEIROS OLIVEIRA, CNPTIA; WENCESLAU GERALDES TEIXEIRA, CNPS; JOAO HERBERT MOREIRA VIANA, CNPMS; ELOI RIBEIRO, ISRIC World Soil Information; CARLOS GUSTAVO TORNQUIST, UFRGS; LÚCIA HELENA CUNHA DOS ANJOS, UFRRJ; JOSÉ JOÃO LELIS LEAL DE SOUZA, UFRGN; MARTA VASCONCELOS OTTONI, Serviço Geológico do Brasil; PAULA SUÉLEN CORRÊA DE MEDEIROS, IBGE; DIEGO JOSÉ GRIS, UFSM; NÍCOLAS AUGUSTO ROSIN, UFSM; JEAN MICHEL MOURA BUENO, UFSM; HUMBERTO GONCALVES DOS SANTOS, CNPS; ELISEU JOSÉ WEBER, UFRGS; CARLOS ALBERTO FLORES, CPACT; ELIAS MENDES COSTA, UFRRJ; RONALDO PEREIRA DE OLIVEIRA, CNPS; JOSE MARIA FILIPPINI ALBA, CPACT; JOÃO CHRISÓSTOMO PEDROSO NETO, Epamig; FABRÍCIO DE ARAÚJO PEDRON, UFSM; JOÃO HENRIQUE CAVIGLIONE, Iapar; GUSTAVO SOUZA VALLADARES, UFPI; CARMEM SUEZE SILVA MIRANDA, Univasf; JOSÉ ALEXANDRE MELO DEMATTÊ, USP; JOSÉ MARQUES JÚNIOR, Unesp; DIEGO SILVA SIQUEIRA, Unesp; RENATO ELEOTERIO DE AQUINO, Unesp; NELIDA ELIZABET QUIÑONEZ SILVERO, Unesp; ALINE MARQUES GENÚ, UNICENTRO; TIAGO BROETTO, Catena Planejamento Territorial; LUCIANO CAMPOS CANCIAN, UFSM; PABLO MIGUEL, UFPel; JOVANI ZALAMENA, UFSC; ANDRÉ CARNIELETTO DOTTO, USP; JAIME ANTONIO DE ALMEIDA, Udesc; JOSÉ MIGUEL REICHERT, UFSM; GUSTAVO RIBAS CURCIO, CNPF; LEONARDO SANTOS COLLIER, UFG; WALDIR DE CARVALHO JUNIOR, CNPS; ADEMIR FONTANA, CNPS; ALINE PACOBAHYBA DE OLIVEIRA, CNPS; EDUARDO SALDANHA VOGELMANN, FURG; FÁBIO JOEL KOCHEM MALLMANN, Universidade Regional Integrada do Alto Uruguai e das Missões; GUSTAVO DE MATTOS VASQUES, CNPS; IGO FERNANDO LEPSCH, USP; JESSÉ RODRIGO FINK, IFPR; JOÃO CARLOS KER, UFV; LEANDRO SOUZA DA SILVA, UFSM; PEDRO LUIZ DE FREITAS, CNPS; WANDERLEI BIELUCZYK, USP; TALES TIECHER, UFRGS. |
Título: |
Bringing together Brazilian soil scientists to share soil data. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
In: WORLD CONGRESS OF SOIL SCIENCE, 21., 2018, Rio de Janeiro. Soil science: beyond food and fuel: abstracts. Viçosa, MG: SBCS, 2018. |
Páginas: |
Não paginado. |
Idioma: |
Inglês |
Notas: |
WCSS 2018. |
Palavras-Chave: |
Anthropic soils; Árvore de decisão; Data mining techniques; Decision tree; Estoque de carbono; Mineração de dados; Soil management system. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02438naa a2200853 a 4500 001 2102011 005 2018-12-19 008 2018 bl --- 0-- u #d 100 1 $aSAMUEL-ROSA, A. 245 $aBringing together Brazilian soil scientists to share soil data.$h[electronic resource] 260 $c2018 300 $aNão paginado. 500 $aWCSS 2018. 653 $aAnthropic soils 653 $aÁrvore de decisão 653 $aData mining techniques 653 $aDecision tree 653 $aEstoque de carbono 653 $aMineração de dados 653 $aSoil management system 700 1 $aDALMOLIN, R. S. D. 700 1 $aGUBIANI, P. I. 700 1 $aOLIVEIRA, S. R. de M. 700 1 $aTEIXEIRA, W. G. 700 1 $aVIANA, J. H. M. 700 1 $aRIBEIRO, E. 700 1 $aTORNQUIST, C. G. 700 1 $aANJOS, L. H. C. dos 700 1 $aSOUZA, J. J. E. L. de 700 1 $aOTTONI, M. V. 700 1 $aMEDEIROS, P. S. C. de 700 1 $aGRIS, D. J. 700 1 $aROSIN, N. A. 700 1 $aBUENO, J. M. M. 700 1 $aSANTOS, H. G. dos 700 1 $aWEBER, E. J. 700 1 $aFLORES, C. A. 700 1 $aCOSTA, E. M. 700 1 $aOLIVEIRA, R. P. de 700 1 $aFILIPPINI ALBA, J. M. 700 1 $aPEDROSO NETO, J. C. 700 1 $aPEDRON, F. de A. 700 1 $aCAVIGLIONE, J. H. 700 1 $aVALLADARES, G. S. 700 1 $aMIRANDA, C. S. S. 700 1 $aDEMATTÊ, J. A. M. 700 1 $aMARQUES JÚNIOR, J. 700 1 $aSIQUEIRA, D. S. 700 1 $aAQUINO, R. E. de 700 1 $aSILVERO, N. E. Q. 700 1 $aGENÚ, A. M. 700 1 $aBROETTO, T. 700 1 $aCANCIAN, L. C. 700 1 $aMIGUEL, P. 700 1 $aZALAMENA, J. 700 1 $aDOTTO, A. C. 700 1 $aALMEIDA, J. A. de 700 1 $aREICHERT. 700 1 $aCURCIO, G. R. 700 1 $aCOLLIER, L. S. 700 1 $aCARVALHO JUNIOR, W. de 700 1 $aFONTANA, A. 700 1 $aOLIVEIRA, A. P. de 700 1 $aVOGELMANN, E. S. 700 1 $aMALLMANN, F. J. K. 700 1 $aVASQUES, G. de M. 700 1 $aLEPSCH, I. F. 700 1 $aFINK, J. R. 700 1 $aKER, J. C. 700 1 $aSILVA, L. S. da 700 1 $aFREITAS, P. L. de 700 1 $aBIELUCZYK, B. 700 1 $aTIECHER, T. 773 $tIn: WORLD CONGRESS OF SOIL SCIENCE, 21., 2018, Rio de Janeiro. Soil science: beyond food and fuel: abstracts. Viçosa, MG: SBCS, 2018.
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