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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
20/07/2009 |
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Data da última atualização: |
11/11/2010 |
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Autoria: |
STANGARLIN, J. R.; PASCHOLATI, S. F.; FRANZENER, G. |
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Afiliação: |
JOSÉ RENATO STANGARLIN, UNIOESTE; SÉRGIO F. PASCHOLATI, ESALQ/USP; GILMAR FRANZENER, UNIOESTE. |
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Título: |
Phenols, B-1,3 glucanase, chitinase and phenylalanine ammonia-lyase activities in infection sites of Exserohilum turcicum in maize genotypes. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Summa Phytopathologica, Botucatu, v. 31, n. 3, p. 261-267, July/Sept. 2005. |
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Idioma: |
Inglês |
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Conteúdo: |
Resistant and susceptible maize inbreds were inoculated with the pathogen Exserohilum turcicum to compare biochemical responses in both interactions. Total phenolic compounds, and the activities of ?-1,3 glucanase, chitinase and phenylalanine ammonia-lyase were measured after inoculation with the pathogen. The production of fungitoxic compounds was evaluated by thin-layer chromatography using tissue samples. Fungal biomass inside infected tissues was determined by using ergosterol quantification as a parameter. The results showed that in the resistant maize inbred line, the infection site (called "primary infection site") was represented by chlorotic spots, with restricted colonization of the xylem vessel and mesophyll cells around it, accompanied by increases in the concentration of phenolic compounds and high ?-1,3 glucanase and chitinase activities. In the susceptible inbred, however, two types of infection sites were characterized. In the first, the primary infection site was represented by chlorotic spots, with moderate ?-1,3 glucanase and chitinase activities and restricted colonization by the fungus. In the second, necrotic lesions (secondary infection site) were seen away from the penetration area and, increases in the content of phenolic compounds, moderate activity of ?-1,3 glucanase and chitinase, and strong colonization ofmesophyll tissue: by the pathogen were detected. These results provide evidence for different patterns of lesion development in resistant and susceptible maize inbred lines following E. turcicum infection. MenosResistant and susceptible maize inbreds were inoculated with the pathogen Exserohilum turcicum to compare biochemical responses in both interactions. Total phenolic compounds, and the activities of ?-1,3 glucanase, chitinase and phenylalanine ammonia-lyase were measured after inoculation with the pathogen. The production of fungitoxic compounds was evaluated by thin-layer chromatography using tissue samples. Fungal biomass inside infected tissues was determined by using ergosterol quantification as a parameter. The results showed that in the resistant maize inbred line, the infection site (called "primary infection site") was represented by chlorotic spots, with restricted colonization of the xylem vessel and mesophyll cells around it, accompanied by increases in the concentration of phenolic compounds and high ?-1,3 glucanase and chitinase activities. In the susceptible inbred, however, two types of infection sites were characterized. In the first, the primary infection site was represented by chlorotic spots, with moderate ?-1,3 glucanase and chitinase activities and restricted colonization by the fungus. In the second, necrotic lesions (secondary infection site) were seen away from the penetration area and, increases in the content of phenolic compounds, moderate activity of ?-1,3 glucanase and chitinase, and strong colonization ofmesophyll tissue: by the pathogen were detected. These results provide evidence for different patterns of lesion development in resistant and s... Mostrar Tudo |
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Palavras-Chave: |
Atividade fungitóxica; Excerohilum turcicum; Híbridos de milho; Sítios de infecção. |
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Thesagro: |
Zea Mays. |
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Categoria do assunto: |
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Marc: |
LEADER 02243naa a2200205 a 4500
001 1083208
005 2010-11-11
008 2005 bl uuuu u00u1 u #d
100 1 $aSTANGARLIN, J. R.
245 $aPhenols, B-1,3 glucanase, chitinase and phenylalanine ammonia-lyase activities in infection sites of Exserohilum turcicum in maize genotypes.
260 $c2005
520 $aResistant and susceptible maize inbreds were inoculated with the pathogen Exserohilum turcicum to compare biochemical responses in both interactions. Total phenolic compounds, and the activities of ?-1,3 glucanase, chitinase and phenylalanine ammonia-lyase were measured after inoculation with the pathogen. The production of fungitoxic compounds was evaluated by thin-layer chromatography using tissue samples. Fungal biomass inside infected tissues was determined by using ergosterol quantification as a parameter. The results showed that in the resistant maize inbred line, the infection site (called "primary infection site") was represented by chlorotic spots, with restricted colonization of the xylem vessel and mesophyll cells around it, accompanied by increases in the concentration of phenolic compounds and high ?-1,3 glucanase and chitinase activities. In the susceptible inbred, however, two types of infection sites were characterized. In the first, the primary infection site was represented by chlorotic spots, with moderate ?-1,3 glucanase and chitinase activities and restricted colonization by the fungus. In the second, necrotic lesions (secondary infection site) were seen away from the penetration area and, increases in the content of phenolic compounds, moderate activity of ?-1,3 glucanase and chitinase, and strong colonization ofmesophyll tissue: by the pathogen were detected. These results provide evidence for different patterns of lesion development in resistant and susceptible maize inbred lines following E. turcicum infection.
650 $aZea Mays
653 $aAtividade fungitóxica
653 $aExcerohilum turcicum
653 $aHíbridos de milho
653 $aSítios de infecção
700 1 $aPASCHOLATI, S. F.
700 1 $aFRANZENER, G.
773 $tSumma Phytopathologica, Botucatu$gv. 31, n. 3, p. 261-267, July/Sept. 2005.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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