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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/07/2013 |
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Data da última atualização: |
18/12/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CALEGARIO, R. F.; LOCALI, E. C.; STACH-MACHADO, D. R.; PERON, L. A.; CASERTA, R.; SALAROLI, R. B.; ASTUA, J. de F.; MACHADO, M. A.; KITAJIMA, E. W. |
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Afiliação: |
RENATA F. CALEGARIO, ESALQ; ELIANE C. LOCALI, APTA; DAGMAR R. STACH-MACHADO; LUÍS ANTÔNIO PERON; RAQUEL CASERTA, APTA; RENATO B. SALAROLI, ESALQ; JULIANA DE FREITAS ASTUA, CNPMF; MARCOS A. MACHADO, APTA; ELLIOT W. KITAJIMA, ESALQ. |
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Título: |
Polyclonal antibodies to the putative coat protein of Citrus leprosis virus C expressed in Escherichia coli: production and use in immunodiagnosis. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v.38 n.3 Brasília May/June 2013. |
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Idioma: |
Inglês |
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Notas: |
Epub May 14, 2013. |
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Conteúdo: |
This work reports the in vitro expression of Citrus leprosis virus C (CiLV-C) putative coat protein (p29) and the production of a polyclonal antibody to be used as a tool for serological diagnosis of citrus leprosis. The ORF2/RNA1, corresponding to p29, was cloned in pET28a and transformed into Escherichia coli cells (BL21). Expression of p29 was induced in vitro and the protein was purified and used for immunization of rabbits to produce the polyclonal antibody. The anti p29 serum was shown to be highly specific to CiLV-C detection by immunological methods (Western blot, PTA-ELISA, tissue blot and in situ immunolocalization), without cross reaction with healthy citrus plants or other cytoplasmic and nuclear viruses transmitted by Brevipalpus mites. These results demonstrate that the antibody against CiLV-C p29 protein is highly specific for CiLV-C detection. In situ immunogold labeling assays on thin sections of sweet orange leaf cells infected by CiLV-C demonstrated that short, bacilliform particles present within cisternae of the endoplasmic reticulum were specifically labeled, confirming their viral nature. The dense cytoplasmic viroplasm was also heavily labeled indicating that it represents a site of p29 accumulation. |
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Thesagro: |
Fruta citríca. |
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Thesaurus Nal: |
Citrus; Citrus leprosis virus C; Electron microscopy; Immunoassays. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02121naa a2200289 a 4500
001 1961973
005 2013-12-18
008 2013 bl uuuu u00u1 u #d
100 1 $aCALEGARIO, R. F.
245 $aPolyclonal antibodies to the putative coat protein of Citrus leprosis virus C expressed in Escherichia coli$bproduction and use in immunodiagnosis.
260 $c2013
500 $aEpub May 14, 2013.
520 $aThis work reports the in vitro expression of Citrus leprosis virus C (CiLV-C) putative coat protein (p29) and the production of a polyclonal antibody to be used as a tool for serological diagnosis of citrus leprosis. The ORF2/RNA1, corresponding to p29, was cloned in pET28a and transformed into Escherichia coli cells (BL21). Expression of p29 was induced in vitro and the protein was purified and used for immunization of rabbits to produce the polyclonal antibody. The anti p29 serum was shown to be highly specific to CiLV-C detection by immunological methods (Western blot, PTA-ELISA, tissue blot and in situ immunolocalization), without cross reaction with healthy citrus plants or other cytoplasmic and nuclear viruses transmitted by Brevipalpus mites. These results demonstrate that the antibody against CiLV-C p29 protein is highly specific for CiLV-C detection. In situ immunogold labeling assays on thin sections of sweet orange leaf cells infected by CiLV-C demonstrated that short, bacilliform particles present within cisternae of the endoplasmic reticulum were specifically labeled, confirming their viral nature. The dense cytoplasmic viroplasm was also heavily labeled indicating that it represents a site of p29 accumulation.
650 $aCitrus
650 $aCitrus leprosis virus C
650 $aElectron microscopy
650 $aImmunoassays
650 $aFruta citríca
700 1 $aLOCALI, E. C.
700 1 $aSTACH-MACHADO, D. R.
700 1 $aPERON, L. A.
700 1 $aCASERTA, R.
700 1 $aSALAROLI, R. B.
700 1 $aASTUA, J. de F.
700 1 $aMACHADO, M. A.
700 1 $aKITAJIMA, E. W.
773 $tTropical Plant Pathology$gv.38 n.3 Brasília May/June 2013.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 4 | |
| 1. |  | CALEGARIO, R. F.; KITAJIMA, E. W.; LOCALI-FABRIS, E. C.; SALAROLI, R. B.; FREITAS-ASTÚA, J.; STACH- MACHADO, D. R.; CASERTA, R.; MACHADO, M. A. In situ immunocytochemistry of citrus leaves infected with Citrus leprosis virus cytoplasmic type (CiLV-C). Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 2. | | CALEGARIO, R. F.; LOCALI, E. C.; STACH-MACHADO, D. R.; PERON, L. A.; CASERTA, R.; SALAROLI, R. B.; ASTUA, J. de F.; MACHADO, M. A.; KITAJIMA, E. W. Polyclonal antibodies to the putative coat protein of Citrus leprosis virus C expressed in Escherichia coli: production and use in immunodiagnosis. Tropical Plant Pathology, v.38 n.3 Brasília May/June 2013. Epub May 14, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 3. | | CALEGARIO, R. F.; LABATE, M. T. V.; PERONI, LUÍS A.; STACH-MACHADO, D. R.; ASTUA, J. de F.; LABATE, C. A.; MACHADO, M. A.; KITAJIMA, E. W. In vitro expression and antiserum production against the movement protein of Citrus leprosis virus C (CiLV-C). Tropical Plant Pathology, n. 2, v. 37, p. 255-265, March-April, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 4. | | MACHADO, M. A.; AMARAL, A. M. do; BASTIANEL, M.; BOSCARIOL-CAMARGO, R. L.; CARLOS, E. F.; DELLA COLETTA FILHO, H.; CRISTOFANI-YALY, M.; FREITAS-ASTÚA, J.; LOCALI-FABRIS, E. C.; NOVELLI, V. M.; PERONI, L. A.; SILVA-PINHATI, A. C.; SOUZA, A. A.; STACH-MACHADO, D. R.; TAKITA, M. A.; TARGON, M. L. P. N.; REIS, M. da S. Expressed citrus sequence (CitEST) database in Brazil: features and tools. In: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 16., 2008, San Diego. Final abstracts guide. San Diego: Illumina, 2008. W105| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 4 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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