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Registro Completo |
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
15/08/2016 |
Data da última atualização: |
15/08/2016 |
Autoria: |
SPANAMBERG, A.; FERREIRO, L.; MACHADO, G.; FRAGA, C. F.; ARAUJO, R. |
Afiliação: |
ANDRÉIA SPANAMBERG, PPGCV/UFRGS; LAERTE FERREIRO, UFRGS; GUSTAVO MACHADO, PPGCV/UFRGS; CIBELE FLORIANO FRAGA, PPGCV/UFRGS; RICARDO ARAUJO, IPATIMUP/Universidade do Porto. |
Título: |
Identification and characterization of Aspergillus fumigatus isolates from broilers. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v.36, n. 7, p. 591-594, jul. 2016. |
Idioma: |
Inglês |
Conteúdo: |
Aspergillosis is one of the main causes of mortality in birds. The pulmonary system is most frequently affected, with lesions observed in the air sacs and lungs of a wide variety of bird species. The aim of this study was to confirm by molecular methods the identification and the genetic diversity of Aspergillus fumigatus isolates of lung?s samples from healthy broilers (Galus galus domesticus). Forty-four (9.5%) isolates of lung?s samples were confirmed as A. fumigatus by polymerase chain reaction (PCR) multiplex (amplification of ?-tub and rodA gene fragments). Microsatellite typing for A. fumigatus was used to analyse all avian isolates. Among them, 40 genotypes (90.9%) were observed only one time. The results showed a high variability and multiple genotypes of de A. fumigatus collected from lung?s samples of broilers. |
Palavras-Chave: |
Genotipagem; Identificação molecular; Molecular identification; Multiplex PCR; PCR multiplex. |
Thesagro: |
Aspergillus Fumigatus; Frango de corte. |
Thesaurus Nal: |
Broiler chickens; Genotyping. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/146359/1/Identification-and-characterization.pdf
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Marc: |
LEADER 01639naa a2200277 a 4500 001 2050857 005 2016-08-15 008 2016 bl uuuu u00u1 u #d 100 1 $aSPANAMBERG, A. 245 $aIdentification and characterization of Aspergillus fumigatus isolates from broilers. 260 $c2016 520 $aAspergillosis is one of the main causes of mortality in birds. The pulmonary system is most frequently affected, with lesions observed in the air sacs and lungs of a wide variety of bird species. The aim of this study was to confirm by molecular methods the identification and the genetic diversity of Aspergillus fumigatus isolates of lung?s samples from healthy broilers (Galus galus domesticus). Forty-four (9.5%) isolates of lung?s samples were confirmed as A. fumigatus by polymerase chain reaction (PCR) multiplex (amplification of ?-tub and rodA gene fragments). Microsatellite typing for A. fumigatus was used to analyse all avian isolates. Among them, 40 genotypes (90.9%) were observed only one time. The results showed a high variability and multiple genotypes of de A. fumigatus collected from lung?s samples of broilers. 650 $aBroiler chickens 650 $aGenotyping 650 $aAspergillus Fumigatus 650 $aFrango de corte 653 $aGenotipagem 653 $aIdentificação molecular 653 $aMolecular identification 653 $aMultiplex PCR 653 $aPCR multiplex 700 1 $aFERREIRO, L. 700 1 $aMACHADO, G. 700 1 $aFRAGA, C. F. 700 1 $aARAUJO, R. 773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv.36, n. 7, p. 591-594, jul. 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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Registros recuperados : 2 | |
1. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SPANAMBERG, A.; FERREIRO, L.; MACHADO, G.; FRAGA, C. F.; ARAUJO, R. Identification and characterization of Aspergillus fumigatus isolates from broilers. Pesquisa Veterinária Brasileira, Brasília, DF, v.36, n. 7, p. 591-594, jul. 2016.Biblioteca(s): Embrapa Unidades Centrais. |
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2. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | CASAGRANDE, R. A.; MACHADO, G.; GUERRA, P. R.; CASTRO, L. A.; SPANAMBERG, A.; SILVA, S. C.; CARDOSO, M. R. de I.; DRIEMEIER, D. Caracterização anatomopatológica e bacteriológica em frangos de corte condenados totalmente por colibacilose sob Serviço de Inspeção Federal. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 9, p. 949-957, setembro. 2017. Título em inglês: Pathological and bacteriological characterization on broilers totally condemned due to colibacillosis under the control of the Federal Inspection Service.Biblioteca(s): Embrapa Unidades Centrais. |
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