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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
17/11/2004 |
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Data da última atualização: |
06/05/2022 |
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Autoria: |
CASTRO, C. S. P. de; SOUZADE, J. R.; BLOCH JUNIOR, C. |
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Título: |
The binding of zinc (II) to a double-stranded oligodeoxyribonucleotide; a voltammetric study. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Biophysical Chemistry, v.112, n. 1, p. 59-67, 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
Binding of zinc to a 19 mer double-stranded oligodeoxyribonucleotide was investigated by anodic stripping voltammetry and cyclic voltammetry in order to understand the roles of zinc in DNA cleavage catalyzed by mung bean nuclease. These methods rely on the direct monitoring of zinc oxidation current in the absence and in the presence of the oligo. Zinc titration curves with the ds-oligodeoxyribonucleotide were obtained in concentrations ranging from 3.62×10-9 to 3.62×10-8 M and 4.06×10-10 to 5.25×10-9 M. The acquired data were used to determine the dissociation constant, stoichiometry and zinc binding sites of the complex and to understand the specific changes of ds-oligodeoxyribonucleotide secondary structure by zinc binding. The oxidation-reduction process of zinc was also investigated by cyclic voltammetry through I (oxidation current) versus v1/2 (square root of scan rate) curves in the absence and in the presence of the double-stranded oligodeoxyribonucleotide. |
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Palavras-Chave: |
Metodos eletroquímicos; Voltametria. |
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Thesagro: |
DNA; Zinco. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/177880/1/ID-24577.pdf
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Marc: |
LEADER 01555naa a2200193 a 4500
001 1185340
005 2022-05-06
008 2004 bl uuuu u00u1 u #d
100 1 $aCASTRO, C. S. P. de
245 $aThe binding of zinc (II) to a double-stranded oligodeoxyribonucleotide; a voltammetric study.$h[electronic resource]
260 $c2004
520 $aBinding of zinc to a 19 mer double-stranded oligodeoxyribonucleotide was investigated by anodic stripping voltammetry and cyclic voltammetry in order to understand the roles of zinc in DNA cleavage catalyzed by mung bean nuclease. These methods rely on the direct monitoring of zinc oxidation current in the absence and in the presence of the oligo. Zinc titration curves with the ds-oligodeoxyribonucleotide were obtained in concentrations ranging from 3.62×10-9 to 3.62×10-8 M and 4.06×10-10 to 5.25×10-9 M. The acquired data were used to determine the dissociation constant, stoichiometry and zinc binding sites of the complex and to understand the specific changes of ds-oligodeoxyribonucleotide secondary structure by zinc binding. The oxidation-reduction process of zinc was also investigated by cyclic voltammetry through I (oxidation current) versus v1/2 (square root of scan rate) curves in the absence and in the presence of the double-stranded oligodeoxyribonucleotide.
650 $aDNA
650 $aZinco
653 $aMetodos eletroquímicos
653 $aVoltametria
700 1 $aSOUZADE, J. R.
700 1 $aBLOCH JUNIOR, C.
773 $tBiophysical Chemistry$gv.112, n. 1, p. 59-67, 2004.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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