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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
03/08/2020 |
Data da última atualização: |
03/08/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BRAIR, V. L.; MAIA, A. L. R. S.; CORREIA, L. F. L.; BARBOSA, N. O.; SANTOS, J. D. R.; BRANDÃO, F. Z.; FONSECA, J. F. da; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G. |
Afiliação: |
VIVIANE L. BRAIR, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; ANA LUCIA R. S. MAIA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; LUCAS FRANCISCO L. CORREIA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; NATHALIA O. BARBOSA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JULIANA D. R. SANTOS, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; FELIPE Z. BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC; RIBRIO IVAN T. P. BATISTA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JOANNA M. G. SOUZA-FABJAN, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil. |
Título: |
Gene expression patterns of in vivo-derived sheep blastocysts is more affected by vitrification than slow freezing technique. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Cryobiology, v. 95, p. 110-115, 2020. |
DOI: |
https://doi.org/10.1016/j.cryobiol.2020.05.009 |
Idioma: |
Inglês |
Conteúdo: |
Transfer of fresh sheep embryos frequently results in higher pregnancy rate compared to cryopreserved ones, possibly due to a failure in the communication between the cryopreserved embryo and the endometrium during pre-implantation and pregnancy establishment. Thus, this study assessed the effect of sheep embryo cryopres-ervation (slow freezing or vitrification) on embryo survival rate and expression of genes related to trophectoderm differentiation (CDX2), pluripotency maintenance (NANOG), cell proliferation (TGFB1), mitochondrial activity (NRF1) and apoptosis (BAX and BCL2). Superovulation (n ¼32 ewes) was performed and embryos were transcervically collected. One hundred good quality (Grade I and II) embryos were allocated into three groups: fresh embryos (CTL; n ¼15), slow freezing (SF; n ¼42) or vitrification (VT; n ¼43). After thawing/warming, three pools of five blastocysts per group were used for RT-qPCR; the remaining 55 embryos were cultured in vitro in SOFaa medium at 38.5 �C and 5% CO2 (SF: n ¼27 and VT: n ¼28). Survival rate of SF and VT were, respectively, 29.6% (8/27) and 14.2% (4/28) at 24 h; and 48.1% (13/27) and 32.1% (9/28) at 48 h (P >0.05). Only CDX2 was affected (up-regulated, P <0.05) in both groups compared to CTL. The BAX transcript was upregulated in VT, compared to SF group. The VT increased (P <0.05) the expression of all genes, except for NANOG and NRF1, when compared to the CTL. In conclusion, although in vitro survival was similar between techniques, VT led to increased changes in blastocyst gene expression compared to CTL and SF. MenosTransfer of fresh sheep embryos frequently results in higher pregnancy rate compared to cryopreserved ones, possibly due to a failure in the communication between the cryopreserved embryo and the endometrium during pre-implantation and pregnancy establishment. Thus, this study assessed the effect of sheep embryo cryopres-ervation (slow freezing or vitrification) on embryo survival rate and expression of genes related to trophectoderm differentiation (CDX2), pluripotency maintenance (NANOG), cell proliferation (TGFB1), mitochondrial activity (NRF1) and apoptosis (BAX and BCL2). Superovulation (n ¼32 ewes) was performed and embryos were transcervically collected. One hundred good quality (Grade I and II) embryos were allocated into three groups: fresh embryos (CTL; n ¼15), slow freezing (SF; n ¼42) or vitrification (VT; n ¼43). After thawing/warming, three pools of five blastocysts per group were used for RT-qPCR; the remaining 55 embryos were cultured in vitro in SOFaa medium at 38.5 �C and 5% CO2 (SF: n ¼27 and VT: n ¼28). Survival rate of SF and VT were, respectively, 29.6% (8/27) and 14.2% (4/28) at 24 h; and 48.1% (13/27) and 32.1% (9/28) at 48 h (P >0.05). Only CDX2 was affected (up-regulated, P <0.05) in both groups compared to CTL. The BAX transcript was upregulated in VT, compared to SF group. The VT increased (P <0.05) the expression of all genes, except for NANOG and NRF1, when compared to the CTL. In conclusion, although in vitro survival was similar between... Mostrar Tudo |
Palavras-Chave: |
Apoptosis regulator; Embryo cryopreservation; Embryonic metabolism; Reproductive biotechnologies. |
Thesaurus Nal: |
Embryo implantation; Embryo transfer; Ewes. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02602naa a2200313 a 4500 001 2124160 005 2020-08-03 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.cryobiol.2020.05.009$2DOI 100 1 $aBRAIR, V. L. 245 $aGene expression patterns of in vivo-derived sheep blastocysts is more affected by vitrification than slow freezing technique.$h[electronic resource] 260 $c2020 520 $aTransfer of fresh sheep embryos frequently results in higher pregnancy rate compared to cryopreserved ones, possibly due to a failure in the communication between the cryopreserved embryo and the endometrium during pre-implantation and pregnancy establishment. Thus, this study assessed the effect of sheep embryo cryopres-ervation (slow freezing or vitrification) on embryo survival rate and expression of genes related to trophectoderm differentiation (CDX2), pluripotency maintenance (NANOG), cell proliferation (TGFB1), mitochondrial activity (NRF1) and apoptosis (BAX and BCL2). Superovulation (n ¼32 ewes) was performed and embryos were transcervically collected. One hundred good quality (Grade I and II) embryos were allocated into three groups: fresh embryos (CTL; n ¼15), slow freezing (SF; n ¼42) or vitrification (VT; n ¼43). After thawing/warming, three pools of five blastocysts per group were used for RT-qPCR; the remaining 55 embryos were cultured in vitro in SOFaa medium at 38.5 �C and 5% CO2 (SF: n ¼27 and VT: n ¼28). Survival rate of SF and VT were, respectively, 29.6% (8/27) and 14.2% (4/28) at 24 h; and 48.1% (13/27) and 32.1% (9/28) at 48 h (P >0.05). Only CDX2 was affected (up-regulated, P <0.05) in both groups compared to CTL. The BAX transcript was upregulated in VT, compared to SF group. The VT increased (P <0.05) the expression of all genes, except for NANOG and NRF1, when compared to the CTL. In conclusion, although in vitro survival was similar between techniques, VT led to increased changes in blastocyst gene expression compared to CTL and SF. 650 $aEmbryo implantation 650 $aEmbryo transfer 650 $aEwes 653 $aApoptosis regulator 653 $aEmbryo cryopreservation 653 $aEmbryonic metabolism 653 $aReproductive biotechnologies 700 1 $aMAIA, A. L. R. S. 700 1 $aCORREIA, L. F. L. 700 1 $aBARBOSA, N. O. 700 1 $aSANTOS, J. D. R. 700 1 $aBRANDÃO, F. Z. 700 1 $aFONSECA, J. F. da 700 1 $aBATISTA, R. I. T. P. 700 1 $aSOUZA-FABJAN, J. M. G. 773 $tCryobiology$gv. 95, p. 110-115, 2020.
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Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
12/04/2010 |
Data da última atualização: |
27/04/2010 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 2 |
Autoria: |
POLTRONIERI, L. S.; FIGUEIREDO, D. V.; BRIOSO, P. S. T.; VERZIGNASSI, J. R.; CARDOSO, S. S. |
Afiliação: |
LUIZ SEBASTIAO POLTRONIERI, CPATU; DANIEL VAZQUEZ FIGUEIREDO, UFRRJ; PAULO SÉRGIO TORRES BRIOSO, UFRRJ; JAQUELINE ROSEMEIRE VERZIGNASSI, CNPGC; SHIRLEY SOUSA CARDOSO, Secretaria de Desenvolvimento, Ciência e Tecnologia do Estado do Pará. |
Título: |
Constatação do Banana streak Uganda B virus em bananeiras no Estado do Pará. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Summa Phytopathologica, Botucatu, v. 35, n. 1, p. 74, jan./feb. 2009. |
DOI: |
10.1590/S0100-54052009000100018 |
Idioma: |
Português |
Palavras-Chave: |
Banana streak Uganda B; Brasil; Pará. |
Thesaurus NAL: |
Amazonia. |
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
Marc: |
LEADER 00655naa a2200217 a 4500 001 1663872 005 2010-04-27 008 2009 bl --- 0-- u #d 024 7 $a10.1590/S0100-54052009000100018$2DOI 100 1 $aPOLTRONIERI, L. S. 245 $aConstatação do Banana streak Uganda B virus em bananeiras no Estado do Pará. 260 $c2009 650 $aAmazonia 653 $aBanana streak Uganda B 653 $aBrasil 653 $aPará 700 1 $aFIGUEIREDO, D. V. 700 1 $aBRIOSO, P. S. T. 700 1 $aVERZIGNASSI, J. R. 700 1 $aCARDOSO, S. S. 773 $tSumma Phytopathologica, Botucatu$gv. 35, n. 1, p. 74, jan./feb. 2009.
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