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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
26/12/2018 |
Data da última atualização: |
09/08/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G.; ALFRADIQUE, V. A. P.; ARASHIRO, E. K. N.; COSENTINO, I. O.; PINTO, P. H. N.; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. |
Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC. |
Título: |
Dose and administration protocol for FSH used for ovarian stimulation affect gene expression in sheep cumulus-oocyte complexes. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 30, n. 9, p. 1234-1244, 2018. |
DOI: |
https://doi.org/10.1071/rd17337 |
Idioma: |
Inglês |
Conteúdo: |
The present study evaluated the effect of four ovarian stimulation protocols on the follicular population and molecular status of cumulus-oocyte complexes (COCs). Twelve Santa Inês ewes (in a cross-over design) received 80 or 120mg FSH alone in a multiple-dose (MD80 and MD120) regimen or in combination with 300IU equine chorionic gonadotrophin (eCG) in a one-shot (OS80 and OS120) protocol. The follicular population, COC recovery rate, mean COCs per ewe and the rate of brilliant Cresyl blue-positive (BCB+) COCs were similar among treatments (P>0.05). The expression of markers of oocyte competence (ZAR1, zygote arrest 1; MATER, maternal antigen that embryo requires; GDF9, growth differentiation factor 9; BMP15, bone morphogenetic protein 15; Bcl-2, B-cell lymphoma 2; BAX, Bcl-2 associated X protein) and the steroidogenic pathway (ER?, oestrogen receptor ?; LHr, LH receptor; FSHr, FSH receptor; STAR, steroidogenic acute regulatory protein) was affected by stimulation. Specifically, the expression of markers of the steroidogenic pathway was reduced with increasing FSH dose in the OS protocol. FSH at a dose of 80mg reduced the expression of FSHr and ER? in the OS versus MD protocol. Conversely, in MD protocol, only LHr was affected by increasing FSH dose. In conclusion, 80mg FSH in the MD or OS protocol was sufficient to promote the development of multiple follicles and obtain fully grown (BCB+) oocytes. The MD protocol may be more appropriate for the production of better-quality oocytes. MenosThe present study evaluated the effect of four ovarian stimulation protocols on the follicular population and molecular status of cumulus-oocyte complexes (COCs). Twelve Santa Inês ewes (in a cross-over design) received 80 or 120mg FSH alone in a multiple-dose (MD80 and MD120) regimen or in combination with 300IU equine chorionic gonadotrophin (eCG) in a one-shot (OS80 and OS120) protocol. The follicular population, COC recovery rate, mean COCs per ewe and the rate of brilliant Cresyl blue-positive (BCB+) COCs were similar among treatments (P>0.05). The expression of markers of oocyte competence (ZAR1, zygote arrest 1; MATER, maternal antigen that embryo requires; GDF9, growth differentiation factor 9; BMP15, bone morphogenetic protein 15; Bcl-2, B-cell lymphoma 2; BAX, Bcl-2 associated X protein) and the steroidogenic pathway (ER?, oestrogen receptor ?; LHr, LH receptor; FSHr, FSH receptor; STAR, steroidogenic acute regulatory protein) was affected by stimulation. Specifically, the expression of markers of the steroidogenic pathway was reduced with increasing FSH dose in the OS protocol. FSH at a dose of 80mg reduced the expression of FSHr and ER? in the OS versus MD protocol. Conversely, in MD protocol, only LHr was affected by increasing FSH dose. In conclusion, 80mg FSH in the MD or OS protocol was sufficient to promote the development of multiple follicles and obtain fully grown (BCB+) oocytes. The MD protocol may be more appropriate for the production of better-quality... Mostrar Tudo |
Palavras-Chave: |
Oócito. |
Thesagro: |
Biologia Molecular; Ovelha. |
Thesaurus Nal: |
Molecular biology; Oocytes. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02470naa a2200301 a 4500 001 2102508 005 2022-08-09 008 2018 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1071/rd17337$2DOI 100 1 $aBRAGANÇA, G. M. 245 $aDose and administration protocol for FSH used for ovarian stimulation affect gene expression in sheep cumulus-oocyte complexes.$h[electronic resource] 260 $c2018 520 $aThe present study evaluated the effect of four ovarian stimulation protocols on the follicular population and molecular status of cumulus-oocyte complexes (COCs). Twelve Santa Inês ewes (in a cross-over design) received 80 or 120mg FSH alone in a multiple-dose (MD80 and MD120) regimen or in combination with 300IU equine chorionic gonadotrophin (eCG) in a one-shot (OS80 and OS120) protocol. The follicular population, COC recovery rate, mean COCs per ewe and the rate of brilliant Cresyl blue-positive (BCB+) COCs were similar among treatments (P>0.05). The expression of markers of oocyte competence (ZAR1, zygote arrest 1; MATER, maternal antigen that embryo requires; GDF9, growth differentiation factor 9; BMP15, bone morphogenetic protein 15; Bcl-2, B-cell lymphoma 2; BAX, Bcl-2 associated X protein) and the steroidogenic pathway (ER?, oestrogen receptor ?; LHr, LH receptor; FSHr, FSH receptor; STAR, steroidogenic acute regulatory protein) was affected by stimulation. Specifically, the expression of markers of the steroidogenic pathway was reduced with increasing FSH dose in the OS protocol. FSH at a dose of 80mg reduced the expression of FSHr and ER? in the OS versus MD protocol. Conversely, in MD protocol, only LHr was affected by increasing FSH dose. In conclusion, 80mg FSH in the MD or OS protocol was sufficient to promote the development of multiple follicles and obtain fully grown (BCB+) oocytes. The MD protocol may be more appropriate for the production of better-quality oocytes. 650 $aMolecular biology 650 $aOocytes 650 $aBiologia Molecular 650 $aOvelha 653 $aOócito 700 1 $aBATISTA, R. I. T. P. 700 1 $aSOUZA-FABJAN, J. M. G. 700 1 $aALFRADIQUE, V. A. P. 700 1 $aARASHIRO, E. K. N. 700 1 $aCOSENTINO, I. O. 700 1 $aPINTO, P. H. N. 700 1 $aCAMARGO, L. S. de A. 700 1 $aFONSECA, J. F. da 700 1 $aBRANDÃO, F. Z. 773 $tReproduction, Fertility and Development$gv. 30, n. 9, p. 1234-1244, 2018.
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61. | | OLIVEIRA, M. A. S.; ALVES, R. T.; SOUZA, M. A.; PIZARRO, E. A. Efeito da cigarrinha-das-pastagens Deois flavopicta (Stal, 1854) no comportamento de diferentes ecotipos de Brachiaria spp no Distrito Federal. In: REUNION SABANAS, 1., 1992, Brasilia, DF. Red Internacional de Evaluacion de Pastos Tropicales - RIEPT. Brasilia: EMBRAPA-CPAC / [Cali]: CIAT, 1992. p.501-505.Biblioteca(s): Embrapa Cerrados. |
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63. | | PIZARRO, E. A.; AYARZA, M. A.; SPAIN, J. M.; CARVALHO, M. A.; SOUZA, M. A. de. Efecto de la irrigacion en la produccion de semillas de Stylosanthes guianensis cv. Mineirao. Pasturas Tropicales, Cali, v.15, n.3, p.27-28, 1993.Biblioteca(s): Embrapa Cerrados. |
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64. | | CARVALHO, M. A.; KORNELIUS, R.; PIZARRO, E. A.; VALLS, J. F. M.; VILLELA, L. Efeito de epocas, metodos e taxas de semeadura no estabelecimento de Paspalum atratum Swallen. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 34., 1997, Juiz de Fora, MG. Anais... Juiz de Fora: SBZ, 1997. v.2. p.193-195.Biblioteca(s): Embrapa Cerrados. |
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68. | | RAMOS, A. K. B.; PIZARRO, E. A.; CARVALHO, M. A.; VALLS, J. F. M.; RODRIGUES, L. R. de A. Avaliacao agronomica de genotipos de "Paspalum" spp. no ambito dos cerrados. I. Condicao de baixa fertilidade inicial do solo. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 40., 2003, Santa Maria, RS. Otimizando a producao animal: anais. Santa Maria: SBZ: UFSM, 2003. 1 CD-ROM.Biblioteca(s): Embrapa Cerrados. |
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70. | | RAMOS, A. K. B.; PIZARRO, E. A.; CARVALHO, M. A.; VALLS, J. F. M.; RODRIGUES, L. R. de A. Avaliacao agronomica de genotipos de "Paspalum" spp. no ambito dos cerrados. II. Condicao de alta fertilidade inicial do solo. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 40., 2003, Santa Maria, RS. Otimizando a producao animal: anais. Santa Maria: SBZ: UFSM, 2003. 1 CD-ROM.Biblioteca(s): Embrapa Cerrados. |
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73. | | KERRIDGE, P. C.; PIZARRO, E. A.; VALLS, J. F. M.; ARGEL, P. J.; BARCELLOS, A.; MAASS, B. L. Genetic improvement through exploiting natural diversity. In: SIMPOSIO SOBRE O CERRADO, 8., 1996. Brasilia, DF. Anais do 8. Simposio sobre o cerrado: biodiversidade e producao sustentavel de alimentos e fibras nos cerrados. Planaltina, DF: EMBRAPA-CPAC, 1996. p.311. p.311Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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74. | | KERRIDGE, P. C.; PIZARRO, E. A.; VALLS, J. F. M.; ARGEL, P. J.; BARCELLOS, A.; MAASS, B. L. Genetic improvement through exploiting natural diversity. In: SIMPOSIO SOBRE O CERRADO, 8.; INTERNATIONAL SYMPOSIUM ON TROPICAL SAVANNAS, 1., 1996, Brasilia, DF. Biodiversidade e producao sustentavel de alimentos e fibras nos Cerrados: anais... Planaltina: EMBRAPA-CPAC, 1996. p.311.Biblioteca(s): Embrapa Cerrados. |
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75. | | FONSECA, L. de M.; PIZARRO, E. A.; RAMOS, A. K. B.; BARCELLOS, A. de O.; CARVALHO, M. A. Taxa de semeadura de Arachis pintoi para formacao de pastagem em solo de varzea. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 33., 1996, Fortaleza, CE. Anais... Fortaleza: SBZ, 1996. v.2. p.389-391.Biblioteca(s): Embrapa Cerrados. |
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76. | | GOIS, S. L. L. de; VILELA, L.; PIZARRO, E. A.; CARVALHO, M. A.; RAMOS, A. K. B. Efeito de calcario, fosforo e potassio na producao de forragem de Arachis pintoi. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 33., 1996, Fortaleza, CE. Anais... Fortaleza: SBZ, 1996. v.2 p.73-74.Biblioteca(s): Embrapa Cerrados. |
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77. | | GOIS, S. L. L. de; VILELA, L.; PIZARRO, E. A.; CARVALHO, M. A.; RAMOS, A. K. B. Efeito de calcario, fosforo e potassio na producao de forragem de Arachis pintoi. Pasturas Tropicales, Cali, v.19, n.3, p.9-13, 1997.Biblioteca(s): Embrapa Cerrados. |
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78. | | PIZARRO, E. A.; VALLS, J. F.; RAMOS, A. K. B.; GODOY, I. J.; CARVALHO, M. A.; SINGH, A. K. Potencial forrajero de Arachis hypogaea en el cerrado brasileno. Pasturas Tropicales, Cali, v.18, n.3, p.17-24, 1996.Biblioteca(s): Embrapa Cerrados. |
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Registros recuperados : 80 | |
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