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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
30/11/2021 |
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Data da última atualização: |
01/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FONSECA, J. F. da; VERGANI, G. B.; LIMA, M. S. D.; SILVA, K. de M.; MONTEIRO, A. W. U.; RAMOS, A. F.; ALVES, B. R. C.; SOUZA-FABJAN, J. M. G.; OLIVEIRA, M. E. F.; BATISTA, R. I. T. P. |
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Afiliação: |
JEFERSON FERREIRA DA FONSECA, CNPC; GABRIEL B. VERGANI, Universidade Estadual Paulista (UNESP) - Jaboticabal, SP, Brazil; MONALISA S. D. LIMA, Universidade Estadual do Ceará (UFC) - Fortaleza, CE, Brazil; KLEIBE DE MORAES SILVA, CNPC; ALEXANDRE WEICK UCHOA MONTEIRO, CNPC; ALEXANDRE FLORIANI RAMOS, Cenargen; BRUNA R. C. ALVES; JOANNA M. G. SOUZA-FABJAN; MARIA E. F. OLIVEIRA; RIBRIO I. T. P. BATISTA, Universidade Federal Fluminense (UFF) - Niterói, Brazil. |
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Título: |
Nonsurgical embryo recovery as a feasible tool for supporting embryo biobanks of Locally Adapted Brazilian sheep and goats. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Biopreserv Biobank, Nov. 2021. |
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Descrição Física: |
Online ahead of print. |
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DOI: |
doi: 10.1089/bio.2021.0066. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study assessed the outcomes of nonsurgical embryo recovery (NSER) after superovulation (SOV) in five locally adapted Brazilian breeds of sheep and goats. The objective was to evaluate the feasibility and efficiency of using SOV combined with a less-invasive embryo collection technique for supplying the Brazilian animal gene bank with germplasm from specific genotypes of interest. Morada Nova (n = 20), Santa Inês (n = 20), and Somalis (n = 20) ewes received an intravaginal progesterone (330 mg) device for 9 days, while Canindé (n = 15) and Moxotó (n = 15) goats received an intravaginal medroxyprogesterone acetate (60 mg) device for 6 days. All females received 133 mg of porcine follicle-stimulating hormone (pFSH) administrated in six decreasing doses 12 hours apart, starting 60 hours before device removal, plus 37.5 ?g of d-cloprostenol at the fifth and sixth pFSH dose. Donors in estrus were mated with fertile males. The corpora lutea (CL) number was assessed by ultrasonography 1 day before NSER. On day 6.5 or 7 after estrus, NSER was performed following hormonally induced cervical relaxation. A total of 97% of sheep and 90% of goats responded with estrus, and among those, 91% of sheep and 85% of goats presented a CL. In ewes, the numbers of CL were greater (p < 0.05) in the Santa Inês breed, while similar (p > 0.05) CL numbers were found among the goat breeds. All viable embryos were freezable (excellent and good quality) and the number per donor was 7.8 for sheep and 4.9 for goats. All parameters of NSER efficiency, embryo yield, and fertility post-NSER did not differ (p > 0.05) between breeds among each species. The SOV-NSER procedures applied for an embryo biobank supply of locally adapted Brazilian breeds of small ruminants were efficient regarding production of cryopreservable embryos, and preservation of donor fertility. Therefore, SOV followed by NSER is recommended for embryo biobank assembly in sheep and goats. MenosAbstract: This study assessed the outcomes of nonsurgical embryo recovery (NSER) after superovulation (SOV) in five locally adapted Brazilian breeds of sheep and goats. The objective was to evaluate the feasibility and efficiency of using SOV combined with a less-invasive embryo collection technique for supplying the Brazilian animal gene bank with germplasm from specific genotypes of interest. Morada Nova (n = 20), Santa Inês (n = 20), and Somalis (n = 20) ewes received an intravaginal progesterone (330 mg) device for 9 days, while Canindé (n = 15) and Moxotó (n = 15) goats received an intravaginal medroxyprogesterone acetate (60 mg) device for 6 days. All females received 133 mg of porcine follicle-stimulating hormone (pFSH) administrated in six decreasing doses 12 hours apart, starting 60 hours before device removal, plus 37.5 ?g of d-cloprostenol at the fifth and sixth pFSH dose. Donors in estrus were mated with fertile males. The corpora lutea (CL) number was assessed by ultrasonography 1 day before NSER. On day 6.5 or 7 after estrus, NSER was performed following hormonally induced cervical relaxation. A total of 97% of sheep and 90% of goats responded with estrus, and among those, 91% of sheep and 85% of goats presented a CL. In ewes, the numbers of CL were greater (p < 0.05) in the Santa Inês breed, while similar (p > 0.05) CL numbers were found among the goat breeds. All viable embryos were freezable (excellent and good quality) and the number per donor was 7.8 for s... Mostrar Tudo |
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Palavras-Chave: |
Brazilian Somalis; Canindé; Genetic resources conservation; Morada Nova; Moxotó; NSER; Santa Inês; Transcervical. |
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Thesaurus Nal: |
Animal genetic resources; Animal reproduction; Breeding and Genetic Improvement; Cryopreservation; Embryo transfer; Small ruminants; Superovulation. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03262naa a2200433 a 4500
001 2136840
005 2021-12-01
008 2021 bl uuuu u00u1 u #d
024 7 $adoi: 10.1089/bio.2021.0066.$2DOI
100 1 $aFONSECA, J. F. da
245 $aNonsurgical embryo recovery as a feasible tool for supporting embryo biobanks of Locally Adapted Brazilian sheep and goats.$h[electronic resource]
260 $c2021
300 $cOnline ahead of print.
520 $aAbstract: This study assessed the outcomes of nonsurgical embryo recovery (NSER) after superovulation (SOV) in five locally adapted Brazilian breeds of sheep and goats. The objective was to evaluate the feasibility and efficiency of using SOV combined with a less-invasive embryo collection technique for supplying the Brazilian animal gene bank with germplasm from specific genotypes of interest. Morada Nova (n = 20), Santa Inês (n = 20), and Somalis (n = 20) ewes received an intravaginal progesterone (330 mg) device for 9 days, while Canindé (n = 15) and Moxotó (n = 15) goats received an intravaginal medroxyprogesterone acetate (60 mg) device for 6 days. All females received 133 mg of porcine follicle-stimulating hormone (pFSH) administrated in six decreasing doses 12 hours apart, starting 60 hours before device removal, plus 37.5 ?g of d-cloprostenol at the fifth and sixth pFSH dose. Donors in estrus were mated with fertile males. The corpora lutea (CL) number was assessed by ultrasonography 1 day before NSER. On day 6.5 or 7 after estrus, NSER was performed following hormonally induced cervical relaxation. A total of 97% of sheep and 90% of goats responded with estrus, and among those, 91% of sheep and 85% of goats presented a CL. In ewes, the numbers of CL were greater (p < 0.05) in the Santa Inês breed, while similar (p > 0.05) CL numbers were found among the goat breeds. All viable embryos were freezable (excellent and good quality) and the number per donor was 7.8 for sheep and 4.9 for goats. All parameters of NSER efficiency, embryo yield, and fertility post-NSER did not differ (p > 0.05) between breeds among each species. The SOV-NSER procedures applied for an embryo biobank supply of locally adapted Brazilian breeds of small ruminants were efficient regarding production of cryopreservable embryos, and preservation of donor fertility. Therefore, SOV followed by NSER is recommended for embryo biobank assembly in sheep and goats.
650 $aAnimal genetic resources
650 $aAnimal reproduction
650 $aBreeding and Genetic Improvement
650 $aCryopreservation
650 $aEmbryo transfer
650 $aSmall ruminants
650 $aSuperovulation
653 $aBrazilian Somalis
653 $aCanindé
653 $aGenetic resources conservation
653 $aMorada Nova
653 $aMoxotó
653 $aNSER
653 $aSanta Inês
653 $aTranscervical
700 1 $aVERGANI, G. B.
700 1 $aLIMA, M. S. D.
700 1 $aSILVA, K. de M.
700 1 $aMONTEIRO, A. W. U.
700 1 $aRAMOS, A. F.
700 1 $aALVES, B. R. C.
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aOLIVEIRA, M. E. F.
700 1 $aBATISTA, R. I. T. P.
773 $tBiopreserv Biobank, Nov. 2021.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
19/12/2005 |
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Data da última atualização: |
26/03/2012 |
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Autoria: |
PEDRINI, M. R. da S.; MEDEIROS, U. K. L. de; ANDRADE JUNIOR, W. M. de; XAVIER, C. H.; SOLDÓRIO, I. L.; PARO, F. E.; MOSCARDI, F.; SOUZA, M. L de; MEDEIROS, M. de F. D. de. |
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Título: |
Drying of Baculovirus anticarsia biopesticide formulations in spouted bed of inert particles. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
In: MERCOSUR CONGRESS ON CHEMICAL ENGINEERING, 2.; MERCOSUR CONGRESS ON PROCESS SYSTEMS ENGINEERING, 2., 2005, Rio de Janeiro. Proceedings... Rio de Janeiro: UFRJ, 2005. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
Microorganisms are used for biological control of pests and are considered less aggressive to the environment than chemical pesticides. Research with Anticarsia gemmatalis nucleopolyhedrovirus (AgNPV) has led to the wide scale use of this pathogen in Brazil, over an estimated 2 million hectares of soybean fields annually. The spouted bed with inert particles has been used to dry pastes, such as suspensions and sludges. This work is aimed to test the application of spouted bed dryer for AgNPV biopesticide production. Experiments were carried out using two levels of airflow rate (10 e 11m/s) e air temperature (70 e 80 ºC). The airflow rate influenced positively promoting a 25% increase in the powder production efficiency. It was noted a 5% decrease in the powder production efficiency when 80 ºC was used. Higher airflow produced more humid powders and higher temperatures produced driest powders, where the average powder moisture obtained was 8,6 %. Lethal concentration for 50% of the test population (LC50) was used to evaluate the biological activity and Transmission Electron Microscopy (TEM) was carried out to evaluate biopesticide integrity. No damage was observed in the dried biopesticide and no significant difference was observed between viral biological activities of dried samples compared with a standard after 11 days of infection.These results indicate that spouted bed can be considered a viable alternative for baculovirus biopesticide formulation production. |
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Thesagro: |
Engenharia. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02306naa a2200241 a 4500
001 1468656
005 2012-03-26
008 2005 bl uuuu u00u1 u #d
100 1 $aPEDRINI, M. R. da S.
245 $aDrying of Baculovirus anticarsia biopesticide formulations in spouted bed of inert particles.
260 $c2005
300 $c1 CD-ROM.
520 $aMicroorganisms are used for biological control of pests and are considered less aggressive to the environment than chemical pesticides. Research with Anticarsia gemmatalis nucleopolyhedrovirus (AgNPV) has led to the wide scale use of this pathogen in Brazil, over an estimated 2 million hectares of soybean fields annually. The spouted bed with inert particles has been used to dry pastes, such as suspensions and sludges. This work is aimed to test the application of spouted bed dryer for AgNPV biopesticide production. Experiments were carried out using two levels of airflow rate (10 e 11m/s) e air temperature (70 e 80 ºC). The airflow rate influenced positively promoting a 25% increase in the powder production efficiency. It was noted a 5% decrease in the powder production efficiency when 80 ºC was used. Higher airflow produced more humid powders and higher temperatures produced driest powders, where the average powder moisture obtained was 8,6 %. Lethal concentration for 50% of the test population (LC50) was used to evaluate the biological activity and Transmission Electron Microscopy (TEM) was carried out to evaluate biopesticide integrity. No damage was observed in the dried biopesticide and no significant difference was observed between viral biological activities of dried samples compared with a standard after 11 days of infection.These results indicate that spouted bed can be considered a viable alternative for baculovirus biopesticide formulation production.
650 $aEngenharia
700 1 $aMEDEIROS, U. K. L. de
700 1 $aANDRADE JUNIOR, W. M. de
700 1 $aXAVIER, C. H.
700 1 $aSOLDÓRIO, I. L.
700 1 $aPARO, F. E.
700 1 $aMOSCARDI, F.
700 1 $aSOUZA, M. L de
700 1 $aMEDEIROS, M. de F. D. de
773 $tIn: MERCOSUR CONGRESS ON CHEMICAL ENGINEERING, 2.; MERCOSUR CONGRESS ON PROCESS SYSTEMS ENGINEERING, 2., 2005, Rio de Janeiro. Proceedings... Rio de Janeiro: UFRJ, 2005.
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