Data/hora: 15/06/2024 00:52:23

Biblioteca(s): 	Embrapa Caprinos e Ovinos; Embrapa Gado de Leite.	
Data corrente: 	11/08/2022	
Data da última atualização: 	11/08/2022	
Autoria: 	DIAS, J. H.; GONCALVES, J. D.; ARRAIS, A. M.; SOUZA-FABJAN, J. M. G.; BASTOS R.; BATISTA, R. I. T. P.; SIQUEIRA, L. G. B.; OLIVEIRA, M. E. F.; FONSECA, J. F. da.	
Afiliação: 	JENNIFFER HAUSCHILDT DIAS, Universidade Federal de Viçosa; JOEDSON DANTAS GONÇALVES, Universidade Estadual Paulista; ALINE MATOS ARRAIS, Universidade Federal Rural do Rio de Janeiro; JOANNA MARIA GONSALVES SOUZA-FABJAN, Universidade Federal Fluminense; RODOLFO BASTOS, Universidade Estadual do Norte Fluminense Darcy; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MARIA ERICA F. OLIVEIRA, Universidade Estadual Paulista; JEFERSON FERREIRA DA FONSECA, CNPC.	
Título: 	Effects of different doses of estradiol benzoate used in a cervical relaxation protocol on the success of non-surgical embryo recovery and luteal function in superovulated ewes.	
Ano de publicação: 	2023	
Fonte/Imprenta: 	Domestic Animal Endocrinology, v. 82, 106751, 2023.	
DOI: 	https://doi.org/10.1016/j.domaniend.2022.106751	
Idioma: 	Inglês	
Conteúdo: 	This study investigated the effectiveness of different doses of estradiol benzoate (EB) to promote cervical relaxation and their effects on luteal function and outcomes of non-surgical embryo recovery (NSER) in sheep. Multiparous (MULT) and nulliparous (NULL) crossbred Lacaune X Santa Inês ewes were superovulated and naturally bred. Seven days after progesterone withdrawal, females were randomly assigned to one of three distinct cervical relaxation protocols, consisting of i.m. treatment with 37.5 &#956;g d-cloprostenol and different doses of EB: 0.0 mg (0.0EB group; n = 3 NULL and 14 MULT); 0.5 mg (0.5EB group; n = 4 NULL and 12 MULT) or 1.0 mg (1.0EB group, n = 6 NULL and 11 MULT) 16 h before NSER. All ewes received 50 IU of oxytocin 20 min before NSER (D17). Blood samples were collected and ultrasound exams (B-mode and color Doppler) were performed at two timepoints: immediately before d-cloprostenol and EB treatments and prior to NSER. Estrous behavior, corpora lutea count and NSER success outcomes were not affected by EB treatments nor parity (P > 0.05). Embryo recovery rate was greater for ewes in the 0.5EB group and in the NULL ewes (P < 0.05). Ovarian biometrics differed between the two evaluation timepoints in all groups (P < 0.05). Plasma estradiol increased over time, reaching a significant greater level in 1.0EB ewes compared to controls on D17 (P < 0.05), whereas progesterone concentrations decreased over time in all groups (P > 0.05). In conclusion, treatments did not affect NSER success but they did affect luteal function by altering P4 and E2 concentrations.Therefore, the NSER technique can be successfully performed in ewes with or without prior treatment with EB.	
Palavras-Chave: 	Cervical dilation; Dilatação cervical; In vivo embryo production; Non surgical embryo recovery; NSER; Superovulated females.	
Thesagro: 	Ovelha; Ovino; Progesterona.	
Thesaurus Nal: 	Animal reproduction; Embryo transfer; Progesterone; Sheep; Superovulation.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (25738-1) - UPC - AP - DD -  -  -  - DIS
CNPC - (40357-1) - UPC - AP - DD -  -  -  - DIS