Data/hora: 14/06/2024 19:38:37

Biblioteca(s): | Embrapa Caprinos e Ovinos; Embrapa Gado de Leite.
Data corrente: | 08/02/2019
Data da última atualização: | 04/02/2020
Autoria: | FONSECA, J. F. da; ZAMBRINI, F. N.; GUIMARÃES, J. D.; SILVA, M. R.; OLIVEIRA, M. E. F.; BRANDÃO, F. Z.; BARTLEWSKI, P. M.; SOUZA-FABJAN, J. M. G.
Afiliação: | JEFERSON FERREIRA DA FONSECA, CNPC; FABIANA NUNES ZAMBRINI, Universidade Federal de Viçosa (UFV) - Viçosa, MG, Brazil; JOSÉ DOMINGOS GUIMARÃES, Universidade Federal de Viçosa (UFV) - Viçosa, MG, Brazil; MARCIO ROBERTO SILVA, CNPGL; MARIA EMILIA FRANCO OLIVEIRA, Universidade Estadual Paulista (UNESP) - Jaboticabal, SP, Brazil; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; PAWEL MIECZYSLAW BARTLEWSKI, University of Guelph, Guelph, Ontario, Canada; JOANNA MARIA GONÇALVES SOUZA-FABJAN, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.
Título: | Combined treatment with oestradiol benzoate, d-cloprostenol and oxytocin permits cervical dilation and nonsurgical embryo recovery in ewes.
Ano de publicação: | 2019
Fonte/Imprenta: | Reproduction in Domestic Animals, v. 54, n. 1, p. 118-125, Sept. 2019.
DOI: | http://dx.doi.org/10.1111/rda.13318
Idioma: | Inglês
Conteúdo: | Contents This study examined the feasibility of transcervical embryo recovery after the hormonal treatment to induce cervical dilation, following the 7&#8208;day oestrous synchronization protocol in multiparous Santa Inês ewes. A total of 23 cyclic ewes received two doses of 37.5 &#956;g of d&#8208;cloprostenol by latero&#8208;vulvar route 7 days apart. After the second injection of d&#8208;cloprostenol, the ewes were checked for oestrus (every 12 hr) and then mated by fertile rams throughout the oestrous period. All ewes received 37.5 &#956;g of d&#8208;cloprostenol (latero&#8208;vulvar) and 1 mg of oestradiol benzoate by either intramuscular (EBim group; n = 12) or intravaginal (EBivg group; n = 11) route 16 hr before embryo flushing. Twenty minutes before the flushing, 50 IU of oxytocin were administered intravenously. The oestrous response (i.e., the percentage of ewes that showed signs of oestrous behaviour after the second d&#8208;cloprostenol injection) was 91.3% (21/23). The proportion of successfully penetrated ewes (81.8% compared with 80.0%), the mean duration of embryo flushing (24.7 ± 2.0 min compared 26.2 ± 1.9 min), the flushing fluid recovery rate (94.8 ± 1.3% compared with 91.0 ± 2.9%) and the average number of structures recovered per ewe (0.5 ± 0.4 compared with 0.8 ± 0.4) did not vary (p > 0.05) between the EBim and EBivg groups. Viable embryos were recovered from 41.2% (7/17) of successfully penetrated ewes. It can be concluded that nonsurgical (i.e., transcervical) embryo collection can be performed in oestrous&#8208;synchronized Santa Inês ewes pretreated with d&#8208;cloprostenol, oxytocin and oestradiol benzoate, with the latter hormone administered by either the intramuscular or intravaginal route.
Palavras-Chave: | Cervical dilation; Drugs effects; Oestradiol benzoate; Synchronized females; Transcervical embryo collection.
Thesagro: | Estradiol; Hormônio; Ovino; Reprodução Animal; Transferência de Embrião.
Thesaurus Nal: | Cloprostenol; Oxytocin; Sheep.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Caprinos e Ovinos (CNPC)

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CNPGL - (24615-1) - UPC - AP - DD -  -  -  - DIS