Data/hora: 21/09/2024 12:21:46

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	18/10/2010	
Data da última atualização: 	22/08/2022	
Autoria: 	DITA, M. A.; WAALWIJK, C.; BUDDENHAGEN, I. M.; SOUZA JUNIOR, M. T.; KEMA, G. H. J.	
Afiliação: 	MIGUEL ANGEL DITA RODRIGUEZ, CNPMF; C. Waalwijk, Plant Research International; I. W. Buddenhagen, Plum Lane; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE; Gert H. J. Kema, Plant Research International.	
Título: 	A molecular diagnostic for tropical race 4 of the banana fusarium wilt pathogen.	
Ano de publicação: 	2010	
Fonte/Imprenta: 	Plant Pathology, Dordrecht, v. 59, p. 348?357, 2010.	
DOI: 	10.1111/j.1365-3059.2009.02221.x	
Idioma: 	Inglês	
Notas: 	Disponível em: <http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.2009.02221.x/pdf> Acesso em: 26 out. 2010.	
Conteúdo: 	This study analysed genomic variation of the translation elongation factor 1a (TEF-1a) and the intergenic spacer region (IGS) of the nuclear ribosomal operon of Fusarium oxysporum f. sp. cubense (Foc) isolates, from different banana production areas, representing strains within the known races, comprising 20 vegetative compatibility groups (VCG). Based on two single nucleotide polymorphisms present in the IGS region, a PCR-based diagnostic tool was developed to specifically detect isolates from VCG 01213, also called tropical race 4 (TR4), which is currently a major concern in global banana production. Validation involved TR4 isolates, as well as Foc isolates from 19 other VCGs, other fungal plant pathogens and DNA samples from infected tissues of the Cavendish banana cultivar Grand Naine (AAA). Subsequently, a multiplex PCR was developed for fungal or plant samples that also discriminated Musa acuminata and M. balbisiana genotypes. It was concluded that this diagnostic procedure is currently the best option for the rapid and reliable detection and monitoring of TR4 to support eradication and quarantine strategies.	
Palavras-Chave: 	Musa spp.	
Thesagro: 	Banana; Doença de Planta; Fungo; Fusarium Oxysporum; Mal do Panamá.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (26932-1) - UPC - AP - DD -  -  - 12891 - DIS