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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
13/10/2020 |
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Data da última atualização: |
19/03/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TEIXEIRA, N. C.; WENDLAND, A.; OLIVEIRA, M. I. de S.; BRANDÃO, L. T. D.; SOUZA, T. L. P. O. de; FARIA, J. C. de; DEL PELOSO, M. J.; CORTES, M. V. de C. B. |
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Afiliação: |
NARA CRISTINA TEIXEIRA, bolsista CNPAF; ADRIANE WENDLAND FERREIRA, CNPAF; MAYTHSULENE INACIO DE SOUZA OLIVEIRA, bolsista CNPAF; LIVIA TEIXEIRA DUARTE BRANDAO, CNPAF; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; JOSIAS CORREA DE FARIA, CNPAF; MARIA JOSE DEL PELOSO, CNPAF; MARCIO VINICIUS DE C BARROS CORTES, CNPAF. |
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Título: |
Rapid identification of RNA-interference-based resistance to Bean golden mosaic virus in transgenic common beans via loop-mediated isothermal amplification. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Crop Science, v. 60, n. 6, p. 3004-3012, Nov./Dec. 2020. |
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ISSN: |
1435-0653 |
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DOI: |
https://doi.org/10.1002/csc2.20107 |
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Idioma: |
Inglês |
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Conteúdo: |
Amolecular toolwas adapted for practical identification of theworld?s first transgenic event (Embrapa 5.1) in common bean (Phaseolus vulgaris L.). Based on the technology of RNA interference and on the transformation of plants via the Biobalistic method, a transgenic bean was created with effective resistance to Bean golden mosaic virus, its principal virus. To support the monitoring of this technology, the development of new cultivars, and the process of producing, benefiting, and distributing of seeds and grains, a molecular detection tool based on loop-mediated isothermal amplification (LAMP) was developed. This tool can facilitating diagnosis, making it fast, specific, low-cost, and easily executed. Based on the gene Ahas (GenBank M88686) of the Embrapa 5.1 event, sets of initiators were planned (forward outer primer?backward outer primer and forward inner primer?backward inner primer) that are characteristic of the LAMP method. These were evaluated for their sensitivity and specificity in detecting their target. The LAMP method allows direct visual interpretation. The Neutral Red (NR) pH indicator was adopted to facilitate molecular diagnosis in moderately well equipped environments, thus reducing the risk of contamination and the need for skilled labor. This agility in diagnosis occurs because several phases commonly used inmolecular techniques, such as polymerase chain reaction, can be excluded. The LAMPmethod, combined with a rapid DNA extraction method (modified NaOH) and the addition of NR, provides technological support for addressing issues of biosafety, traceability, and identification of the Embrapa 5.1 event in beans in the field and in industry. MenosAmolecular toolwas adapted for practical identification of theworld?s first transgenic event (Embrapa 5.1) in common bean (Phaseolus vulgaris L.). Based on the technology of RNA interference and on the transformation of plants via the Biobalistic method, a transgenic bean was created with effective resistance to Bean golden mosaic virus, its principal virus. To support the monitoring of this technology, the development of new cultivars, and the process of producing, benefiting, and distributing of seeds and grains, a molecular detection tool based on loop-mediated isothermal amplification (LAMP) was developed. This tool can facilitating diagnosis, making it fast, specific, low-cost, and easily executed. Based on the gene Ahas (GenBank M88686) of the Embrapa 5.1 event, sets of initiators were planned (forward outer primer?backward outer primer and forward inner primer?backward inner primer) that are characteristic of the LAMP method. These were evaluated for their sensitivity and specificity in detecting their target. The LAMP method allows direct visual interpretation. The Neutral Red (NR) pH indicator was adopted to facilitate molecular diagnosis in moderately well equipped environments, thus reducing the risk of contamination and the need for skilled labor. This agility in diagnosis occurs because several phases commonly used inmolecular techniques, such as polymerase chain reaction, can be excluded. The LAMPmethod, combined with a rapid DNA extraction method (modified NaO... Mostrar Tudo |
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Thesagro: |
DNA; Feijão; Mosaico Dourado; Phaseolus Vulgaris; Planta Transgênica. |
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Thesaurus Nal: |
Bean golden mosaic virus; Beans; Loop-mediated isothermal amplification; RNA interference; Transgenic plants. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02809naa a2200349 a 4500
001 2125441
005 2021-03-19
008 2020 bl uuuu u00u1 u #d
022 $a1435-0653
024 7 $ahttps://doi.org/10.1002/csc2.20107$2DOI
100 1 $aTEIXEIRA, N. C.
245 $aRapid identification of RNA-interference-based resistance to Bean golden mosaic virus in transgenic common beans via loop-mediated isothermal amplification.$h[electronic resource]
260 $c2020
520 $aAmolecular toolwas adapted for practical identification of theworld?s first transgenic event (Embrapa 5.1) in common bean (Phaseolus vulgaris L.). Based on the technology of RNA interference and on the transformation of plants via the Biobalistic method, a transgenic bean was created with effective resistance to Bean golden mosaic virus, its principal virus. To support the monitoring of this technology, the development of new cultivars, and the process of producing, benefiting, and distributing of seeds and grains, a molecular detection tool based on loop-mediated isothermal amplification (LAMP) was developed. This tool can facilitating diagnosis, making it fast, specific, low-cost, and easily executed. Based on the gene Ahas (GenBank M88686) of the Embrapa 5.1 event, sets of initiators were planned (forward outer primer?backward outer primer and forward inner primer?backward inner primer) that are characteristic of the LAMP method. These were evaluated for their sensitivity and specificity in detecting their target. The LAMP method allows direct visual interpretation. The Neutral Red (NR) pH indicator was adopted to facilitate molecular diagnosis in moderately well equipped environments, thus reducing the risk of contamination and the need for skilled labor. This agility in diagnosis occurs because several phases commonly used inmolecular techniques, such as polymerase chain reaction, can be excluded. The LAMPmethod, combined with a rapid DNA extraction method (modified NaOH) and the addition of NR, provides technological support for addressing issues of biosafety, traceability, and identification of the Embrapa 5.1 event in beans in the field and in industry.
650 $aBean golden mosaic virus
650 $aBeans
650 $aLoop-mediated isothermal amplification
650 $aRNA interference
650 $aTransgenic plants
650 $aDNA
650 $aFeijão
650 $aMosaico Dourado
650 $aPhaseolus Vulgaris
650 $aPlanta Transgênica
700 1 $aWENDLAND, A.
700 1 $aOLIVEIRA, M. I. de S.
700 1 $aBRANDÃO, L. T. D.
700 1 $aSOUZA, T. L. P. O. de
700 1 $aFARIA, J. C. de
700 1 $aDEL PELOSO, M. J.
700 1 $aCORTES, M. V. de C. B.
773 $tCrop Science$gv. 60, n. 6, p. 3004-3012, Nov./Dec. 2020.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
22/10/2021 |
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Data da última atualização: |
22/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PINTO, V. B.; FERREIRA, P. G.; VIDIGAL, P. M. P.; MENDES, T. A. de O.; DAL-BIANCO, M.; MAGALHAES, J. V. de; VIANA, J. M. S. |
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Afiliação: |
VITOR BATISTA PINTO, Universidade Federal de Viçosa; PRISCILA GONÇALVES FERREIRA, Universidade Federal de Viçosa; PEDRO MARCUS PEREIRA VIDIGAL, Universidade Federal de Viçosa; TIAGO ANTÔNIO DE OLIVEIRA MENDES, Universidade Federal de Viçosa; MAXIMILLER DAL-BIANCO, Universidade Federal de Viçosa; JURANDIR VIEIRA DE MAGALHAES, CNPMS; JOSÉ MARCELO SORIANO VIANA, Universidade Federal de Viçosa. |
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Título: |
Uncovering the transcriptional response of popcorn (Zea mays L. var. everta) under long-term aluminum toxicity. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Scientific Reports, v. 11, 19644, 2021. |
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DOI: |
https://doi.org/10.1038/s41598-021-99097-z |
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Idioma: |
Inglês |
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Conteúdo: |
To date, the investigation of genes involved in Al resistance has focused mainly on microarrays and short periods of Al exposure. We investigated genes involved in the global response under Al stress by tracking the expression profile of two inbred popcorn lines with different Al sensitivity during 72 h of Al stress. A total of 1003 differentially expressed genes were identified in the Al-sensitive line, and 1751 were identified in the Al-resistant line, of which 273 were shared in both lines. Genes in the category of ?response to abiotic stress? were present in both lines, but there was a higher number in the Al-resistant line. Transcription factors, genes involved in fatty acid biosynthesis, and genes involved in cell wall modifications were also detected. In the Al-resistant line, GST6 was identified as one of the key hub genes by co-expression network analysis, and ABC6 may play a role in the downstream regulation of CASP-like 5. In addition, we suggest a class of SWEET transporters that might be involved in the regulation of vacuolar sugar storage and may serve as mechanisms for Al resistance. The results and conclusions expand our understanding of the complex mechanisms involved in Al toxicity and provide a platform for future functional analyses and genomic studies of Al stress in popcorn. |
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Palavras-Chave: |
Toxicidade. |
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Thesagro: |
Alumínio; Gene; Milho Pipoca. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227107/1/Uncovering-transcriptional.pdf
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Marc: |
LEADER 02081naa a2200253 a 4500
001 2135487
005 2021-10-22
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/s41598-021-99097-z$2DOI
100 1 $aPINTO, V. B.
245 $aUncovering the transcriptional response of popcorn (Zea mays L. var. everta) under long-term aluminum toxicity.$h[electronic resource]
260 $c2021
520 $aTo date, the investigation of genes involved in Al resistance has focused mainly on microarrays and short periods of Al exposure. We investigated genes involved in the global response under Al stress by tracking the expression profile of two inbred popcorn lines with different Al sensitivity during 72 h of Al stress. A total of 1003 differentially expressed genes were identified in the Al-sensitive line, and 1751 were identified in the Al-resistant line, of which 273 were shared in both lines. Genes in the category of ?response to abiotic stress? were present in both lines, but there was a higher number in the Al-resistant line. Transcription factors, genes involved in fatty acid biosynthesis, and genes involved in cell wall modifications were also detected. In the Al-resistant line, GST6 was identified as one of the key hub genes by co-expression network analysis, and ABC6 may play a role in the downstream regulation of CASP-like 5. In addition, we suggest a class of SWEET transporters that might be involved in the regulation of vacuolar sugar storage and may serve as mechanisms for Al resistance. The results and conclusions expand our understanding of the complex mechanisms involved in Al toxicity and provide a platform for future functional analyses and genomic studies of Al stress in popcorn.
650 $aAlumínio
650 $aGene
650 $aMilho Pipoca
653 $aToxicidade
700 1 $aFERREIRA, P. G.
700 1 $aVIDIGAL, P. M. P.
700 1 $aMENDES, T. A. de O.
700 1 $aDAL-BIANCO, M.
700 1 $aMAGALHAES, J. V. de
700 1 $aVIANA, J. M. S.
773 $tScientific Reports$gv. 11, 19644, 2021.
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Embrapa Milho e Sorgo (CNPMS) |
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