|
|
Registro Completo |
Biblioteca(s): |
Embrapa Soja; Embrapa Suínos e Aves. |
Data corrente: |
06/12/2018 |
Data da última atualização: |
10/12/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SOUZA, R. C.; CANTAO, M. E.; NOGUEIRA, M. A.; VASCONCELOS, A. T. R.; HUNGRIA, M. |
Afiliação: |
RENATA CAROLINI SOUZA, CNPq; MAURICIO EGIDIO CANTAO, CNPSA; MARCO ANTONIO NOGUEIRA, CNPSO; ANA TEREZA RIBEIRO VASCONCELOS, CNPq; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
Título: |
Outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 49, p. 723-730, 2018. |
DOI: |
10.1016/j.bjm.2018.03.001. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The soil represents the main source of novel biocatalysts and biomolecules of industrial relevance. We searched for hydrolases in silico in four shotgun metagenomes (4,079,223 sequences) obtained in a 13-year field trial carried out in southern Brazil, under the no-tillage (NT), or conventional tillage (CT) managements, with crop succession (CS, soybean/wheat), or crop rotation (CR, soybean/maize/wheat/lupine/oat). We identified 42,631 hydrolases belonging to five classes by comparing with the KEGG database, and 44,928 sequences by comparing with the NCBI-NR database. The abundance followed the order: lipases > laccases > cellulases > proteases > amylases > pectinases. Statistically significant differences were attributed to the tillage system, with the NT showing about five times more hydrolases than the CT system. The outstanding differences can be attributed to the management of crop residues, left on the soil surface in the NT, and mechanically broken and incorporated into the soil in the CT. Differences between the CS and the CR were slighter, 10% higher for the CS, but not statistically different. Most of the sequences belonged to fungi (Verticillium, and Colletotrichum for lipases and laccases, and Aspergillus for proteases), and to the archaea Sulfolobus acidocaldarius for amylases. Our results indicate that agricultural soils under conservative managements may represent a hotspotfor bioprospection of hydrolases. |
Palavras-Chave: |
Metagenome. |
Thesagro: |
Manejo do Solo; Microbiologia do Solo; Plantio Direto. |
Thesaurus Nal: |
Metagenomics; Microbiome; No-tillage; Soil enzymes; Soil management. |
Categoria do assunto: |
-- X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/187857/1/final8874.pdf
|
Marc: |
LEADER 02322naa a2200289 a 4500 001 2100838 005 2018-12-10 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1016/j.bjm.2018.03.001.$2DOI 100 1 $aSOUZA, R. C. 245 $aOutstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach.$h[electronic resource] 260 $c2018 520 $aAbstract: The soil represents the main source of novel biocatalysts and biomolecules of industrial relevance. We searched for hydrolases in silico in four shotgun metagenomes (4,079,223 sequences) obtained in a 13-year field trial carried out in southern Brazil, under the no-tillage (NT), or conventional tillage (CT) managements, with crop succession (CS, soybean/wheat), or crop rotation (CR, soybean/maize/wheat/lupine/oat). We identified 42,631 hydrolases belonging to five classes by comparing with the KEGG database, and 44,928 sequences by comparing with the NCBI-NR database. The abundance followed the order: lipases > laccases > cellulases > proteases > amylases > pectinases. Statistically significant differences were attributed to the tillage system, with the NT showing about five times more hydrolases than the CT system. The outstanding differences can be attributed to the management of crop residues, left on the soil surface in the NT, and mechanically broken and incorporated into the soil in the CT. Differences between the CS and the CR were slighter, 10% higher for the CS, but not statistically different. Most of the sequences belonged to fungi (Verticillium, and Colletotrichum for lipases and laccases, and Aspergillus for proteases), and to the archaea Sulfolobus acidocaldarius for amylases. Our results indicate that agricultural soils under conservative managements may represent a hotspotfor bioprospection of hydrolases. 650 $aMetagenomics 650 $aMicrobiome 650 $aNo-tillage 650 $aSoil enzymes 650 $aSoil management 650 $aManejo do Solo 650 $aMicrobiologia do Solo 650 $aPlantio Direto 653 $aMetagenome 700 1 $aCANTAO, M. E. 700 1 $aNOGUEIRA, M. A. 700 1 $aVASCONCELOS, A. T. R. 700 1 $aHUNGRIA, M. 773 $tBrazilian Journal of Microbiology$gv. 49, p. 723-730, 2018.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Suínos e Aves (CNPSA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
07/04/2004 |
Data da última atualização: |
11/09/2013 |
Autoria: |
LEMOS, N. G.; YAMAGUCHI-SHINOZAKI, K.; NAKASHIMA, K.; MOLINA, J. C.; STOLF, R.; MORALES, A. M. R.; LUGLE, S. M.; SILVEIRA, C. A. de; MARIN, S. R. R.; BINNECK, E.; FARIAS, J. R. B.; NEUMAIER, N.; NEPOMUCENO, A. L. |
Título: |
Introduction of genes that confer drought stress tolerance in soybean [Glycine max (L.) Merril] by biobalistic. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
Páginas: |
p. 256. |
Série: |
(Embrapa Soja. Documentos, 228). |
Idioma: |
Inglês |
Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. |
Conteúdo: |
The soybean crop moves the Brazilian economy from the primary sector to the industry, generating many direct and indirect jobs. However, yearly billions of dolars are lost due abiotic and biotic stresses occurring during the crop cycle. Abiotic stresses, precisely drought, is one of the main causes of yield losses. Aiming to minimize this damage, scientists are using biotechnology methods, introducing genes in plants to improve tolerance to drought stress. The plant drought response is regulated by a complex of genes generating a variety of tolerance mecanisms. The complexity of such responses make difficult of using classic breeding methods in the development tolerant varieties. However, genetic engineering of key genes in key metabolic pathways has succeded in incresing drought tolerance in many crops. Our objective was to introduce in soybean the expression cassets pRD29:DREB1 and pE35S:P5CS-129A, using an Embrapa's patent method of Biobalistics that confer dehydration tolerance in plants. DREB1A (Dehidration Responsive Biding Element Protein) is a transcription factor responsible by the activation of genes involved in the drought, freezing and saline stress response; P5CS-129A is also a key enzime in the pathway for proline production. Proline increasing in the cytosol has been related to the cell turgor preservation and protection of cell structures during dehydration by scavenging oxidative radicals. The transformed plants have been generated and tested by PCR. More than 2590 embryos were transformed until now. Nineteen plants were PCR positives for the AHAS gene and none PCR positive plants were detected until now for the p35S:P5CS-129A construct. Number of insertion copies and expression levels will be tested in the positive plants. Physiological characterization in drought conditions will be carried on in promissing events. MenosThe soybean crop moves the Brazilian economy from the primary sector to the industry, generating many direct and indirect jobs. However, yearly billions of dolars are lost due abiotic and biotic stresses occurring during the crop cycle. Abiotic stresses, precisely drought, is one of the main causes of yield losses. Aiming to minimize this damage, scientists are using biotechnology methods, introducing genes in plants to improve tolerance to drought stress. The plant drought response is regulated by a complex of genes generating a variety of tolerance mecanisms. The complexity of such responses make difficult of using classic breeding methods in the development tolerant varieties. However, genetic engineering of key genes in key metabolic pathways has succeded in incresing drought tolerance in many crops. Our objective was to introduce in soybean the expression cassets pRD29:DREB1 and pE35S:P5CS-129A, using an Embrapa's patent method of Biobalistics that confer dehydration tolerance in plants. DREB1A (Dehidration Responsive Biding Element Protein) is a transcription factor responsible by the activation of genes involved in the drought, freezing and saline stress response; P5CS-129A is also a key enzime in the pathway for proline production. Proline increasing in the cytosol has been related to the cell turgor preservation and protection of cell structures during dehydration by scavenging oxidative radicals. The transformed plants have been generated and tested by PCR. More th... Mostrar Tudo |
Thesagro: |
Soja. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03105naa a2200313 a 4500 001 1466831 005 2013-09-11 008 2004 bl uuuu u00u1 u #d 100 1 $aLEMOS, N. G. 245 $aIntroduction of genes that confer drought stress tolerance in soybean [Glycine max (L.) Merril] by biobalistic. 260 $c2004 300 $ap. 256. 490 $a(Embrapa Soja. Documentos, 228). 500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. 520 $aThe soybean crop moves the Brazilian economy from the primary sector to the industry, generating many direct and indirect jobs. However, yearly billions of dolars are lost due abiotic and biotic stresses occurring during the crop cycle. Abiotic stresses, precisely drought, is one of the main causes of yield losses. Aiming to minimize this damage, scientists are using biotechnology methods, introducing genes in plants to improve tolerance to drought stress. The plant drought response is regulated by a complex of genes generating a variety of tolerance mecanisms. The complexity of such responses make difficult of using classic breeding methods in the development tolerant varieties. However, genetic engineering of key genes in key metabolic pathways has succeded in incresing drought tolerance in many crops. Our objective was to introduce in soybean the expression cassets pRD29:DREB1 and pE35S:P5CS-129A, using an Embrapa's patent method of Biobalistics that confer dehydration tolerance in plants. DREB1A (Dehidration Responsive Biding Element Protein) is a transcription factor responsible by the activation of genes involved in the drought, freezing and saline stress response; P5CS-129A is also a key enzime in the pathway for proline production. Proline increasing in the cytosol has been related to the cell turgor preservation and protection of cell structures during dehydration by scavenging oxidative radicals. The transformed plants have been generated and tested by PCR. More than 2590 embryos were transformed until now. Nineteen plants were PCR positives for the AHAS gene and none PCR positive plants were detected until now for the p35S:P5CS-129A construct. Number of insertion copies and expression levels will be tested in the positive plants. Physiological characterization in drought conditions will be carried on in promissing events. 650 $aSoja 700 1 $aYAMAGUCHI-SHINOZAKI, K. 700 1 $aNAKASHIMA, K. 700 1 $aMOLINA, J. C. 700 1 $aSTOLF, R. 700 1 $aMORALES, A. M. R. 700 1 $aLUGLE, S. M. 700 1 $aSILVEIRA, C. A. de 700 1 $aMARIN, S. R. R. 700 1 $aBINNECK, E. 700 1 $aFARIAS, J. R. B. 700 1 $aNEUMAIER, N. 700 1 $aNEPOMUCENO, A. L. 773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Soja (CNPSO) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|