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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
17/12/2009 |
Data da última atualização: |
19/03/2010 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
RAMOS, C. A. N.; ARAÚJO, F. R.; SOUZA, I. I. F.; OLIVEIRA, R. H. M. de; ELISEI, C.; SOARES, C. O.; SACCO, A. M. S.; ROSINHA, G. M. S.; ALVES, L. C. |
Afiliação: |
CARLOS ALBERTO DO NASCIMENTO RAMOS, UFRPE; FLABIO RIBEIRO ARAUJO, CNPGC; INGRID IEDA FERNANDO DE SOUZA, BOLSISTA - CNPGC; RENATO HENRIQUE MARCAL DE OLIVEIRA, CNPGC; CARINA ELISEI, BOLSISTA - CNPGC; CLEBER OLIVEIRA SOARES, CNPGC; ANA MARIA SASTRE SACCO, CPPSUL; GRACIA MARIA SOARES ROSINHA, CNPGC; LEUCIO C. ALVES, UFRPE. |
Título: |
Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n.7, p. 998-1002, nov. 2009. |
Idioma: |
Inglês |
Conteúdo: |
Babesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovis as well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis. The aims of the present paper were to analyse the genetic conservation of the P0 gene among Brazilian isolates of B. bovis and produce recombinant P0 (rPO) from a Brazilian isolate and evaluate its antigenicity with sera from cattle naturally or experimentally infected with B. bovis and sera from cattle experimentally infected with B. bigemina in an enzyme-linked immunosorbent assay (ELISA). MenosBabesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovis as well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis. The aims of the present paper were to analyse the genetic conservation of the P0 gene among Brazilian isolates of B. bovis and produce recombinant P0 (rPO) from a Brazilian isolate and evaluate its antigenicity with sera from cattle naturally or experimentally infected with B. bovis and sera from cattle experimentally infected with B. bige... Mostrar Tudo |
Palavras-Chave: |
Babesiose bovina. |
Thesagro: |
Babesia bovis; Bovino; Sanidade animal. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02329naa a2200265 a 4500 001 1578251 005 2010-03-19 008 2009 bl --- 0-- u #d 100 1 $aRAMOS, C. A. N. 245 $aMolecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis. 260 $c2009 520 $aBabesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovis as well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis. The aims of the present paper were to analyse the genetic conservation of the P0 gene among Brazilian isolates of B. bovis and produce recombinant P0 (rPO) from a Brazilian isolate and evaluate its antigenicity with sera from cattle naturally or experimentally infected with B. bovis and sera from cattle experimentally infected with B. bigemina in an enzyme-linked immunosorbent assay (ELISA). 650 $aBabesia bovis 650 $aBovino 650 $aSanidade animal 653 $aBabesiose bovina 700 1 $aARAÚJO, F. R. 700 1 $aSOUZA, I. I. F. 700 1 $aOLIVEIRA, R. H. M. de 700 1 $aELISEI, C. 700 1 $aSOARES, C. O. 700 1 $aSACCO, A. M. S. 700 1 $aROSINHA, G. M. S. 700 1 $aALVES, L. C. 773 $tMemórias do Instituto Oswaldo Cruz, Rio de Janeiro$gv. 104, n.7, p. 998-1002, nov. 2009.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
14/01/2020 |
Data da última atualização: |
20/01/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
TEODORO, P. E.; FARIAS, F. J. C.; CARVALHO, L. P. de; RIBEIRO, L. P.; NASCIMENTO, M.; AZEVEDO, C. F.; CRUZ, C. D.; BHERING, L. L. |
Afiliação: |
Paulo Eduardo Teodoro, Universidade Federal de Mato Grosso do Sul - UFMS; FRANCISCO JOSE CORREIA FARIAS, CNPA; LUIZ PAULO DE CARVALHO, CNPA; Larissa Pereira Ribeiro, Universidade Federal de Viçosa - UFV; Moysés Nascimento, Universidade Federal de Viçosa - UFV; Camila Ferreira Azevedo, Universidade Federal de Viçosa - UFV; Cosme Damião Cruz, Universidade Federal de Viçosa - UFV; Leonardo Lopes Bhering, Universidade Federal de Viçosa - UFV. |
Título: |
Adaptability and stability of cotton genotypes regarding fiber yield and quality traits. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Crop Science, v. 59, p. 518?524, March?April 2019. |
Idioma: |
Inglês |
Conteúdo: |
The performance of textile processes and the quality of the products depends on the several cotton (Gossypium hirsutum L.) fiber quality traits, such as micronaire index, fiber length, and fiber strength. This research aims to recommend cotton genotypes for the Brazilian Cerrado based on fiber yield and fiber quality traits. Nineteen cotton cultivar variety trials were performed in the 2013?2014 and 2014?2015 crop seasons. Each trial was conducted as a randomized complete block design with 12 treatments and four replications. The evaluated traits were fiber yield, fiber length, fiber strength, and micronaire. For each trait, a modified Lin and Binns method was used to recommend the best genotypes for all environments (favorable and unfavorable). This method was also used in a multivariate manner, aiming at a general recommendation of genotypes based on the multiple traits evaluated. The genotype ´ environment interaction was significant for all evaluated traits. Genotypes with high adaptability and stability were identified for each trait. The multivariate method identified IMA 08 WS and BRS 335 as the genotypes that had the best combination of main desirable traits. The analysis further revealed that new crosses could be performed between genotypes with high yield adaptability and stability and genotypes with adequate fiber quality traits. |
Thesagro: |
Algodão; Fibra; Gossypium Hirsutum. |
Thesaurus NAL: |
Cotton; Fiber quality; Micronaire. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208691/1/Adaptability-and-stability-of-cotton.pdf
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Marc: |
LEADER 02131naa a2200277 a 4500 001 2118672 005 2020-01-20 008 2019 bl uuuu u00u1 u #d 100 1 $aTEODORO, P. E. 245 $aAdaptability and stability of cotton genotypes regarding fiber yield and quality traits.$h[electronic resource] 260 $c2019 520 $aThe performance of textile processes and the quality of the products depends on the several cotton (Gossypium hirsutum L.) fiber quality traits, such as micronaire index, fiber length, and fiber strength. This research aims to recommend cotton genotypes for the Brazilian Cerrado based on fiber yield and fiber quality traits. Nineteen cotton cultivar variety trials were performed in the 2013?2014 and 2014?2015 crop seasons. Each trial was conducted as a randomized complete block design with 12 treatments and four replications. The evaluated traits were fiber yield, fiber length, fiber strength, and micronaire. For each trait, a modified Lin and Binns method was used to recommend the best genotypes for all environments (favorable and unfavorable). This method was also used in a multivariate manner, aiming at a general recommendation of genotypes based on the multiple traits evaluated. The genotype ´ environment interaction was significant for all evaluated traits. Genotypes with high adaptability and stability were identified for each trait. The multivariate method identified IMA 08 WS and BRS 335 as the genotypes that had the best combination of main desirable traits. The analysis further revealed that new crosses could be performed between genotypes with high yield adaptability and stability and genotypes with adequate fiber quality traits. 650 $aCotton 650 $aFiber quality 650 $aMicronaire 650 $aAlgodão 650 $aFibra 650 $aGossypium Hirsutum 700 1 $aFARIAS, F. J. C. 700 1 $aCARVALHO, L. P. de 700 1 $aRIBEIRO, L. P. 700 1 $aNASCIMENTO, M. 700 1 $aAZEVEDO, C. F. 700 1 $aCRUZ, C. D. 700 1 $aBHERING, L. L. 773 $tCrop Science$gv. 59, p. 518?524, March?April 2019.
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Embrapa Algodão (CNPA) |
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