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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
14/05/2004 |
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Data da última atualização: |
14/05/2004 |
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Autoria: |
SALLES, J. F.; SOUZA, F. A. de; VAN ELSAS, J. D. |
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Título: |
Molecular method to assess the diversity of Burkholderia species in environmental. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Applied and Environmental Microbiology, Washington, v. 68, n.4, p. 1595-1603, 2002 |
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Idioma: |
Inglês |
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Conteúdo: |
In spite of the importance of many members of the genus Burkholderia in the soil microbial community, no direct method to assess the diversity of this genus has been developed so far. The aim of this work was the development of soil DNA-based PCR-denaturing gradient gel electrophoresis (DGGE), a powerful tool for studying the diversity of microbial communities, for detection and analysis of the Burkholderia diversity in soil samples. Primers specific for the genus Burkholderia were developed based on the 16S rDNA gene sequence and were evaluated in PCRs performed with genomic DNAs from Burkholderia and non-Burkholderia species as the templates. The primer system used exhibited good specificity and sensitivity for the majority of established species of the genus Burkholderia. DGGE analysis of the PCR products obtained showed that there were sufficient differences in migration behavior to distinguish the majority of the 14 Burkholderia species tested. Sequence analysis of amplicons generated with soil DNA exclusively revealed sequences affiliated with sequence of Burkholderia species, demonstrating that the PCR-DGGE method is suitable for studying the diversity of this genus in natural settings. A PCR-DGGE analysis of the Burkholderia communities en two grassland plots revealed differences in diversity mainly between bulk and rhizosphere soil samples; the communities in the latter samples produced more complex patterns. |
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Thesaurus Nal: |
Burkholderia. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01923naa a2200157 a 4500
001 1625774
005 2004-05-14
008 2002 bl --- 0-- u #d
100 1 $aSALLES, J. F.
245 $aMolecular method to assess the diversity of Burkholderia species in environmental.
260 $c2002
520 $aIn spite of the importance of many members of the genus Burkholderia in the soil microbial community, no direct method to assess the diversity of this genus has been developed so far. The aim of this work was the development of soil DNA-based PCR-denaturing gradient gel electrophoresis (DGGE), a powerful tool for studying the diversity of microbial communities, for detection and analysis of the Burkholderia diversity in soil samples. Primers specific for the genus Burkholderia were developed based on the 16S rDNA gene sequence and were evaluated in PCRs performed with genomic DNAs from Burkholderia and non-Burkholderia species as the templates. The primer system used exhibited good specificity and sensitivity for the majority of established species of the genus Burkholderia. DGGE analysis of the PCR products obtained showed that there were sufficient differences in migration behavior to distinguish the majority of the 14 Burkholderia species tested. Sequence analysis of amplicons generated with soil DNA exclusively revealed sequences affiliated with sequence of Burkholderia species, demonstrating that the PCR-DGGE method is suitable for studying the diversity of this genus in natural settings. A PCR-DGGE analysis of the Burkholderia communities en two grassland plots revealed differences in diversity mainly between bulk and rhizosphere soil samples; the communities in the latter samples produced more complex patterns.
650 $aBurkholderia
700 1 $aSOUZA, F. A. de
700 1 $aVAN ELSAS, J. D.
773 $tApplied and Environmental Microbiology, Washington$gv. 68, n.4, p. 1595-1603, 2002
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 130 | |
| 21. |  | SILVA, I. H. S. da; ARAÚJO, S. N. de; SCHAFFERT, R. E.; SOUZA, F. A. de. Caracterização da resposta a inoculação com Rhizophagus clarus CNPMS 10 em dois genótipos de sorgo contrastantes quanto a eficiência de aquisição de fósforo. In: SIMPÓSIO MINEIRO DE CIÊNCIA DO SOLO, 2., 2013, Viçosa, MG. Fertilidade do solo: conhecimentos, aplicações, interfaces e desafios. Viçosa, MG: Universidade Federal de Viçosa, 2013. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
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| 27. | | SOUZA, F. A. de; STURMER, S. L.; CARRENHO, R.; TRUFEM, S. F. B. Classificação e taxonomia de fungos micorrízicos arbusculares e sua diversidade e ocorrência no Brasil. In: SIQUEIRA, J. O.; SOUZA, F. A. de; CARDOSO, E. J. B. N.; TSAI, S. M. (Ed.). Micorrizas: 30 anos de pesquisas no Brasil. Lavras: UFLA, 2010. cap. 2, p. 15-73.| Tipo: Capítulo em Livro Técnico-Científico |
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| 34. | | OLIVEIRA, E. S.; SOUZA, F. A. de; LIMA, J. E. F. W.; SILVA, E. M. da. Comparação de dois métodos laboratoriais de análise de textura de solos do Cerrado. In: ENCONTRO DE JOVENS TALENTOS DA EMBRAPA CERRADOS, 4., 2009, Planaltina, DF. Resumos apresentados... Planaltina, DF: Embrapa Cerrados, 2009. p. 68-69 (Embrapa Cerrados. Documentos, 243).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 35. | | SIQUEIRA, J. O.; SOUZA, F. A. de; CARDOSO, E. J. B. N.; TSAI, S. M. Histórico e evolução da micorrizologia no Brasil: avanços em três décadas. In: SIQUEIRA, J. O.; SOUZA, F. A. de; CARDOSO, E. J. B. N.; TSAI, S. M. (Ed.). Micorrizas: 30 anos de pesquisas no Brasil. Lavras: UFLA, 2010. cap. 1, p. 1-14.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 130 | |
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