Data/hora: 09/06/2024 03:37:18

Biblioteca(s): | Embrapa Agrobiologia.
Data corrente: | 03/10/2001
Data da última atualização: | 03/10/2001
Autoria: | BENELL, E. M.; BUCK, M.; SOUZA, E. M. de; YATES, M. G.; PEDROSA, F. O.
Título: | Uridylylation of the PII protein from Herbaspirillum seropedicae.
Ano de publicação: | 2001
Fonte/Imprenta: | Canadian Journal of Microbiology, Ottawa, v. 47, p. 309 - 314, 2001.
Idioma: | Inglês
Conteúdo: | The protein is apparently involved in the control of NifA activity in Herbaspirillum seropedicae. To evaluate the probable role of PII in signal transduction, uridylylation assays were conducted with purified H. seropedicae PII and Escherichia coli GlnD, or a cell-free extract of H.seropedicae as sources of uridylylating activity. The results showed that a-ketoglutarate and ATP stimulate uridylylation whereas glutamine inhibits uridylylation. Deuridylylation of PII-UMP was dependent on glutamine and inhibited by ATP and a-ketoglutarate. PII uridylylation and (or) deuridylylation in response to these effectors suggest that PII is a nitrogen level signal transducer in H. seropedicae.
Palavras-Chave: | Growth; Purification.
Thesagro: | Bactéria; Crescimento; Proteína; Purificação.
Thesaurus Nal: | proteins.
Categoria do assunto: | --
Registro original: | Embrapa Agrobiologia (CNPAB)
Exemplares: | CNPAB - (24646-1) - ADD - SP - -- -  -  - 22455 - DIS