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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
08/12/2017 |
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Data da última atualização: |
09/03/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OTTONI, J. R.; CABRAL, L.; SOUSA, S. T. P. de; LACERDA JUNIOR, G. V.; DOMINGOS, D. F.; SOARES JUNIOR, F. L.; SILVA, M. C. P. da; MARCON, J.; DIAS, A. C. F.; MELO, I. S. de; SOUZA, A. P. de; ANDREOTE, F. D.; OLIVEIRA, V. M. de. |
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Afiliação: |
JULIA RONZELLA OTTONI, UNICAMP; LUCELIA CABRAL, UNICAMP; SANDERSON TARCISO PEREIRA DE SOUSA, UNICAMP; GILENO VIEIRA LACERDA JUNIOR, UNICAMP; DANIELA FERREIRA DOMINGOS, UNICAMP; FABIO LINO SOARES JUNIOR, UNICAMP; MYLENNE CALCIOLARI PINHEIRO DA SILVA, ESALQ-USP; JOELMA MARCON, ESALQ-USP; ARMANDO CAVALCANTI FRANCO DIAS, ESALQ-USP; ITAMAR SOARES DE MELO, CNPMA; ANETE PEREIRA DE SOUZA, UNICAMP; FERNANDO DINI ANDREOTE, ESALQ-USP; VALERIA MAIA DE OLIVEIRA, UNICAMP. |
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Título: |
Functional metagenomics of oil-impacted mangrove sediments reveals high abundance of hydrolases of biotechnological interest. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
World Journal of Microbiology and Biotechnology, v. 33, n. 7, p. 1-13, 2017. Article 141. |
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DOI: |
http://dx.doi.org/10.1007/s11274-017-2307-5 |
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Idioma: |
Inglês |
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Conteúdo: |
Mangroves are located in coastal wetlands and are susceptible to the consequences of oil spills, what may threaten the diversity of microorganisms responsible for the nutrient cycling and the consequent ecosystem functioning. Previous reports show that high concentration of oil favors the incidence of epoxide hydrolases and haloalkane dehal- ogenases in mangroves. This finding has guided the goals of this study in an attempt to broaden the analysis to other hydrolases and thereby verify whether oil contamination interferes with the prevalence of particular hydrolases and their assigned microorganisms. For this, an in-depth survey of the taxonomic and functional microbial diversity recov- ered in a fosmid library (Library_Oil Mgv) constructed from oil-impacted Brazilian mangrove sediment was car- ried out. Fosmid DNA of the whole library was extracted and submitted to Illumina HiSeq sequencing. The resulting Library Oil_Mgv dataset was further compared with those obtained by direct sequencing of environmental DNA from Brazilian mangroves (from distinct regions and affected by distinct sources of contamination), focusing on hydro- lases with potential use in biotechnological processes. The most abundant hydrolases found were proteases, esterases and amylases, with similar occurrence profile in all data- sets. The main microbial groups harboring such hydrolase- encoding genes were distinct in each mangrove, and in the fosmid library these enzymes were mainly assigned to Chloroflexaceae (for amylases), Planctomycetaceae (for esterases) and Bradyrhizobiaceae (for proteases). Assem- bly and analysis of Library_Oil Mgv reads revealed three potentially novel enzymes, one epoxide hydrolase, one xylanase and one amylase, to be further investigated via heterologous expression assays. MenosMangroves are located in coastal wetlands and are susceptible to the consequences of oil spills, what may threaten the diversity of microorganisms responsible for the nutrient cycling and the consequent ecosystem functioning. Previous reports show that high concentration of oil favors the incidence of epoxide hydrolases and haloalkane dehal- ogenases in mangroves. This finding has guided the goals of this study in an attempt to broaden the analysis to other hydrolases and thereby verify whether oil contamination interferes with the prevalence of particular hydrolases and their assigned microorganisms. For this, an in-depth survey of the taxonomic and functional microbial diversity recov- ered in a fosmid library (Library_Oil Mgv) constructed from oil-impacted Brazilian mangrove sediment was car- ried out. Fosmid DNA of the whole library was extracted and submitted to Illumina HiSeq sequencing. The resulting Library Oil_Mgv dataset was further compared with those obtained by direct sequencing of environmental DNA from Brazilian mangroves (from distinct regions and affected by distinct sources of contamination), focusing on hydro- lases with potential use in biotechnological processes. The most abundant hydrolases found were proteases, esterases and amylases, with similar occurrence profile in all data- sets. The main microbial groups harboring such hydrolase- encoding genes were distinct in each mangrove, and in the fosmid library these enzymes were mainly assigned to Chlorof... Mostrar Tudo |
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Palavras-Chave: |
Bioprospecting; Fosmid library; Mangrove. |
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Thesagro: |
Hidrolase; Mangue; Microrganismo; Petróleo; Poluição da água. |
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Thesaurus Nal: |
Hydrolases. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02976naa a2200385 a 4500
001 2081939
005 2018-03-09
008 2017 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1007/s11274-017-2307-5$2DOI
100 1 $aOTTONI, J. R.
245 $aFunctional metagenomics of oil-impacted mangrove sediments reveals high abundance of hydrolases of biotechnological interest.$h[electronic resource]
260 $c2017
520 $aMangroves are located in coastal wetlands and are susceptible to the consequences of oil spills, what may threaten the diversity of microorganisms responsible for the nutrient cycling and the consequent ecosystem functioning. Previous reports show that high concentration of oil favors the incidence of epoxide hydrolases and haloalkane dehal- ogenases in mangroves. This finding has guided the goals of this study in an attempt to broaden the analysis to other hydrolases and thereby verify whether oil contamination interferes with the prevalence of particular hydrolases and their assigned microorganisms. For this, an in-depth survey of the taxonomic and functional microbial diversity recov- ered in a fosmid library (Library_Oil Mgv) constructed from oil-impacted Brazilian mangrove sediment was car- ried out. Fosmid DNA of the whole library was extracted and submitted to Illumina HiSeq sequencing. The resulting Library Oil_Mgv dataset was further compared with those obtained by direct sequencing of environmental DNA from Brazilian mangroves (from distinct regions and affected by distinct sources of contamination), focusing on hydro- lases with potential use in biotechnological processes. The most abundant hydrolases found were proteases, esterases and amylases, with similar occurrence profile in all data- sets. The main microbial groups harboring such hydrolase- encoding genes were distinct in each mangrove, and in the fosmid library these enzymes were mainly assigned to Chloroflexaceae (for amylases), Planctomycetaceae (for esterases) and Bradyrhizobiaceae (for proteases). Assem- bly and analysis of Library_Oil Mgv reads revealed three potentially novel enzymes, one epoxide hydrolase, one xylanase and one amylase, to be further investigated via heterologous expression assays.
650 $aHydrolases
650 $aHidrolase
650 $aMangue
650 $aMicrorganismo
650 $aPetróleo
650 $aPoluição da água
653 $aBioprospecting
653 $aFosmid library
653 $aMangrove
700 1 $aCABRAL, L.
700 1 $aSOUSA, S. T. P. de
700 1 $aLACERDA JUNIOR, G. V.
700 1 $aDOMINGOS, D. F.
700 1 $aSOARES JUNIOR, F. L.
700 1 $aSILVA, M. C. P. da
700 1 $aMARCON, J.
700 1 $aDIAS, A. C. F.
700 1 $aMELO, I. S. de
700 1 $aSOUZA, A. P. de
700 1 $aANDREOTE, F. D.
700 1 $aOLIVEIRA, V. M. de
773 $tWorld Journal of Microbiology and Biotechnology$gv. 33, n. 7, p. 1-13, 2017. Article 141.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 306 | |
| 101. |  | CAMARGO, L. S. de A.; QUINTAO, C. C. R.; DENICOL, A. C.; IGUMA, L. T.; CARVALHO, B. C. de; SIQUEIRA, L. G. B.; VIANA, J. H. M. Efeito da tricostatina A na produção de embriões bovinos por transferência nuclear com células somáticas e subsequente gestações. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, 25., 2011, Cumbuco. Resumos... p. 436| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 103. | | VIEIRA, A. C.; QUINTAO, C. C. R.; SIQUEIRA, L. G. B.; OLIVEIRA, C. S.; SILVA, T. A.; VIEIRA, C. D.; CAMARGO, L. S. de A. Effect of epigenetic modulators on development of bovine embryos derived from heatshocked oocytes. Animal Reproduction, v. 15, n. 3, p. 483, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 104. | | QUINTAO, C. C. R.; SOUZA, E. D.; OLIVEIRA, C. S.; FREITAS, C. de; OLIVEIRA, J. A. S.; CAMARGO, L. S. de A. Effect of handmade biopsy on bovine embryons at the sixth day post in vitro fertilization Animal Reproduction., v.12, n.3, Jul./Sept. 2015. Edição dos proceedings do 29º Annual Meeting of the Brazilian Embryo Technology Society, 2015, Gramado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 106. | | COSTA, F. Q.; PEREIRA, M. M.; WOHLRES VIANA, S.; SERAPIAO, R. V.; CARVALHO, B. C.; VIANA, J. H. M.; NOGUEIRA, L. A. G.; CAMARGO, L. S. de A. Effect of heat stress during oocyte maturation on gene expression of in vitro-fertilized and parthenogenetic bovine blastocysts. In: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 107. | | WOHLERS-VIANA, S.; BOITE, M. M.; PEREIRA, M. M.; SA, W. F.; VIANA, J. H. M.; MACHADO, M. A.; CAMARGO, L. S. de A. Effect of production system and breed on relative expression in bovine embryos. In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY - REPRODUCTION, FERTILITY AND DEVELOPMENT., 21., 2009, San Diego. Proceedings... San Diego: Csiro, 2009. 1| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 108. | | WOHLRES-VIANA, S.; BOITE, M. C.; PEREIRA, M. M.; SA, W. F. de; VIANA, J. H. M.; MACHADO, M. A.; CAMARGO, L. S. de A. Effect of production system and breed on relative expression in bovine embryos In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY - REPRODUCTION, FERTILITY AND DEVELOPMENT, 21., 2009, San Diego. Proceeding... San Diego: Csiro, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 109. | | PATROCÍNIOS. T. A.; FERNANDES, C. A. C.; AMORIM, L. S.; RIBEIRO. J. R.; MACEDO, G. C.; SILVA, A. D.; VIANA, J. H. M.; CAMARGO, L. S. de A. Effect of reservatrol analogue on development of in vitro-fertilized bovine embryos. Reproduction, Fertility and Development, v. 29, n. 1, p. 183, 2017. Edição dos proceedings da Annual Conference of the International Embryo Technology Society, Austin, 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 110. | | ASCARI, I. J.; ALVES, N. G.; CAMARGO, L. S. de A.; JASMIN, J.; QUINTAO, C. C. R.; OLIVEIRA, J. A. S.; ARAÚJO, T. D. Effect of melatonin on DNA fragmentation and in vitro maturation of bovine oocytes subjected to heat shock Animal Reproduction., v.12, n.3, Jul./Sept. 2015. Edição dos proceedings do 29º Annual Meeting of the Brazilian Embryo Technology Society, 2015, Gramado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 111. | | PEREIRA, M. M.; MACHADO, M. A.; COSTA, F. de Q.; SERAPIÃO, R. V.; VIANA, J. H. M.; CAMARGO, L. S. de A. Effect of oxygen tension and serum during IVM on developmental competence of bovine oocytes. Reproduction, Fertility and Development, v. 22, p. 1074-1082, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 112. | | CAMARGO, L. S. de A.; SIQUEIRA, L. G. B.; SARAIVA, N. Z.; MAIA, G.; NOGUEIRA, B.; QUINTAO, C. C. R.; OLIVEIRA, C. S. Effect of physiological heat stress on HSP70 and CASPASE 3 levels in bovine oocytes. In: ANNUAL CONFERENCE, 52., 2019, San Jose. Beyond possible: remarkable transformation of reproductive biology: abstracts. San Jose: Society for the Study of Reproduction, 2019.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 113. | | POLISSENI, J.; GRÁZIB, J. G. V. de; GUERRA, M. DE O.; MILEN, L. C.; CAMARGO, L. S. de A.; PETERS, V. M. Impact of the use of gonadotropins on embryonic development - a rat model. JBRA Assisted Reproduction, v. 17, n. 1, p. 32-36, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 114. | | CAMARGO, L. S. de A.; SÁ, W. F. de; FERREIRA, A. de M.; VIANA, J. H. M.; ARAÚJO, M. C. C. Efeito de concentração espermática e período de incubação oócito-espermatozóides na fecundação in vitro em bovinos da raça Gir. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 37, n. 5, p. 709-716, maio 2002 Título em inglês: Effect of sperm concentration and incubation period of oocytespermatozoa on in vitro fertilization in the Gir breed.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 115. | | CAMARGO, L. S. de A.; AGUIRRE-LAVIN, T.; ADENOT, P.; ARAUJO, T. D.; MENDES, V. R. A.; LOURO, I. D.; BEAUJEAN, N.; SOUZA, E. D. Heat shock during in vitro maturation induces chromatin modifications in the bovine embryo. Reproduction, v. 158, n. 4, p. 313-322, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 116. | | CAMARGO, L. S. de A.; GONÇALVES, F. S.; QUINTAO, C. C. R.; IGUMA, L. T.; SOUZA, E. D.; SIQUEIRA, L. G. B.; VIANA, J. H. M. Genetic modification of bovine embryos by lentiviral vectors. Animal Reproduction, v.10, n.3, p. 359, Jul./Sept. 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 117. | | PEREIRA, M. M.; BRANDAO, H. de M.; RAPOSO, N. R.; SERAPIÃO, R. V.; CARVALHO, B. C.; CAMARGO, L. S. de A.; LADEIRA, L. O. Gene expression and apoptosis in bovine preimplantation embryo exposed to carbon nanotubes. In: INTERNATIONAL CONFERENCE ON FOOD AND AGRICULTURE APPLICATIONS OF NANOTECHNOLOGIES, 1., 2010, São Pedro, SP. [Anais...] São Pedro: Aptor Software, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 118. | | GRAZIA, J. G. V.; WOHLRES-VIANA, S.; GARCIA, R. M. G.; MACHADO, M. A.; CAMARGO, L. S. de A.; VIANA, J. H. M. Gene expression in co-cultured granulosa cells from taurine and zebu cattle with high or low rates of in vitro embryo production. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECHNOLOGY SOCIETY, 28., 2014, Natal. Proceedings... [S.l.: s.n.], 2014. p. 428.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 119. | | QUINTAO, C. C. R.; OLIVEIRA, C. S.; FREITAS, C. de; SOUSA, E. D. De; SOUZA, J. F.; ALVES, B. R. C.; CAMARGO, L. S. de A. Desenvolvimento, número de células totais e apoptose em embriões bovinos submetidos à biópsia manual no sétimo dia de fecundação in vitro. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016. 1| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 120. | | CARVALHEIRA, L. de R.; WENCESLAU, R. R.; RIBEIRO, L. dos S.; CARVALHO, B. C. de; BORGES, A. M.; CAMARGO, L. S. de A. Daily vaginal temperature in Girolando cows from three different genetic composition under natural heat stress. Translational Animal Science, v. 5, n. 3, txab138, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 306 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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