Data/hora: 21/09/2024 19:42:45

Biblioteca(s): 	Embrapa Florestas.	
Data corrente: 	05/01/2016	
Data da última atualização: 	04/02/2016	
Autoria: 	CUNICO, M. M.; TREBIEN, H. A.; GALETTI, F. C.; MIGUEL, O. G.; MIGUEL, M. D.; AUER, C. G.; SILVA, C. L.; SOUZA, A. O. de.	
Afiliação: 	MIRIAM M. CUNICO, UFPR; HERBERT A. TREBIEN, UFPR; FÁBIO C. GALETTI, USP; OBDULIO G. MIGUEL, UFPR; MARILIS D. MIGUEL, UFPR; CELSO GARCIA AUER, CNPF; CÉLIO L. SILVA, USP; ANA OLÍVIA DE SOUZA, Instituto Butantan.	
Título: 	Investigation of local anesthetic and antimycobacterial activity of Ottonia martiana Miq. (Piperaceae).	
Ano de publicação: 	2015	
Fonte/Imprenta: 	Anais da Academia Brasileira de Ciências, v. 87, n. 4, p. 1991-2000, 2015.	
DOI: 	http://dx.doi.org/10.1590/0001-3765201520140090	
Idioma: 	Inglês	
Conteúdo: 	Ottonia martiana is a plant popularly known in Brazil by the use for toothache. Ethanolic extract (EE), hexane fraction (HF), dichloromethane fraction (DF) and piperovatine obtained from O. martiana were assayed in vitro and in vivo. The acute toxicity of EE was determined, and LD50 values of 164.5 and 65.0 mg/ kg by the oral and intraperitoneal routes, respectively, indicated a high toxicity for EE in vivo, explaining its popular use by topical administration only. A local anesthetic-like effect of EE and its fractions was observed in experimental models using pain induction, and such effect involved an analgesic action. The antimycobacterial activity of EE, HF, DF and piperovatine was evaluated against Mycobacterium tuberculosis H37Rv ATCC 27924. EE, HF, DF, and piperovatine showed a potential antimycobacterial effect with MICs of 16.0, 62.0, 62.0 and 8.0 ?g/mL, respectively. Piperovatine was more effective than the EE or the other fractions. The selectivity index (SI=IC50/MIC) values calculated for EE, HF, DF and piperovatine based on the MICs and the cytotoxicity against J774 macrophages (IC50 by MTT assay) revealed values of 6.43, 2.34, 1.5 and 9.66, respectively.	
Palavras-Chave: 	Analgesic; Analgésico; Anesthetic; Antimicobacterial; Antimycobacterial; Ottonia martiana; Piperovatina; Piperovatine; Toxicidade.	
Thesagro: 	Anestésico; Espécie Nativa.	
Categoria do assunto: 	--	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/136578/1/2015-Celso-AABC-Investigation.pdf	
Registro original: 	Embrapa Florestas (CNPF)	
Exemplares: 	CNPF - (54653-1) - UPC - AP - DD -  -  -  - DIS