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Registro Completo |
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
04/10/2016 |
Data da última atualização: |
29/12/2017 |
Autoria: |
SOUSA, P. C.; SANTOS, E. A. A.; SILVA, A. M.; BEZERRA, J. A. B.; SOUZA, A. L. P.; LIMA, G. L.; OLIVEIRA, M. F.; SILVA, A. R. |
Afiliação: |
PATRÍCIA C. SOUSA, UFERSA; ERIKA A. A. SANTOS, UFERSA; ANDRÉIA M. SILVA, UFERSA; JOSÉ A. B. BEZERRA, UFERSA; ANA L. P. SOUZA, UFERSA; GABRIELA L. LIMA, UFRR; MOACIR F. OLIVEIRA, UFERSA; ALEXANDRE R. SILVA, UFERSA. |
Título: |
Identification of ultrastructural and functional damage in sperm from six-banded armadillos (Euphractus sexcinctus) due to cryopreservation. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 8, p. 767-774, ago. 2016. |
Idioma: |
Inglês |
Conteúdo: |
The aim of the study was to cryopreserve the semen of six-banded armadillos (Euphractus sexcinctus) in Tris-yolk and glycerol diluent, and to determine the damage caused by the freezing-thawing process, using fluorescent markers and ultrastructural analysis. Semen samples (n=11) collected from 4 adult six-banded armadillos by electroejaculation were cryopreserved in Tris diluent plus 20% egg yolk and 3% glycerol, in a fast freezing curve. Classical analysis of samples was performed after dilution, refrigeration and thawing, followed by fluorescence analysis, using a combination of fluorescent probes to assess membrane integrity (propidium iodide - PI and Hoechst - H342), and mitochondrial activity (CMXRos - Mito Tracker Red®). We also used the ultrastructural analysis to verify possible morphological alterations caused by cryoinjuries. When compared with fresh samples, we verified a significant decline in all the armadillos? semen parameters after thawing, in which only 6.1% motile sperm were found. However, the percentage of sperm which remained with viable (13%) and functional (24.7%) membranes after thawing suggests that some cells could be live but immotile. Analysis using fluorescent markers revealed that the mitochondria of armadillos? sperm is highly sensible to the freezing protocol and the findings through ultrastructure analysis proved this statement. Additionally, the images obtained by transmission electron microscopy revealed that frozenthawed sperm presented damaged plasma membrane, nuclear modifications as changes in chromatin and acrossomal changes relative to sperm capacitation. In conclusion, this study is the first attempt to cryopreserve the semen of an armadillo species, and to help us to identify critical points on the freezing-thawing procedure in order to improve the protocol. MenosThe aim of the study was to cryopreserve the semen of six-banded armadillos (Euphractus sexcinctus) in Tris-yolk and glycerol diluent, and to determine the damage caused by the freezing-thawing process, using fluorescent markers and ultrastructural analysis. Semen samples (n=11) collected from 4 adult six-banded armadillos by electroejaculation were cryopreserved in Tris diluent plus 20% egg yolk and 3% glycerol, in a fast freezing curve. Classical analysis of samples was performed after dilution, refrigeration and thawing, followed by fluorescence analysis, using a combination of fluorescent probes to assess membrane integrity (propidium iodide - PI and Hoechst - H342), and mitochondrial activity (CMXRos - Mito Tracker Red®). We also used the ultrastructural analysis to verify possible morphological alterations caused by cryoinjuries. When compared with fresh samples, we verified a significant decline in all the armadillos? semen parameters after thawing, in which only 6.1% motile sperm were found. However, the percentage of sperm which remained with viable (13%) and functional (24.7%) membranes after thawing suggests that some cells could be live but immotile. Analysis using fluorescent markers revealed that the mitochondria of armadillos? sperm is highly sensible to the freezing protocol and the findings through ultrastructure analysis proved this statement. Additionally, the images obtained by transmission electron microscopy revealed that frozenthawed sperm presented da... Mostrar Tudo |
Palavras-Chave: |
Dano ultraestrutural e funcional; Euphractus sexcinctus; Membrane integrity; Mitochondrial activity; Six-banded armadillo; Sperm; Tatu-peba; Ultraestrutura; Ultrastructural and functional damages; Xenarthras. |
Thesagro: |
Criopreservação; Espermatozoide; Membrana; Mitocondria; Sêmen. |
Thesaurus Nal: |
Cryopreservation; Spermatozoa; Ultrastructure. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/148299/1/Identification-of-ultrastructural.pdf
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Marc: |
LEADER 03089naa a2200421 a 4500 001 2054060 005 2017-12-29 008 2016 bl uuuu u00u1 u #d 100 1 $aSOUSA, P. C. 245 $aIdentification of ultrastructural and functional damage in sperm from six-banded armadillos (Euphractus sexcinctus) due to cryopreservation. 260 $c2016 520 $aThe aim of the study was to cryopreserve the semen of six-banded armadillos (Euphractus sexcinctus) in Tris-yolk and glycerol diluent, and to determine the damage caused by the freezing-thawing process, using fluorescent markers and ultrastructural analysis. Semen samples (n=11) collected from 4 adult six-banded armadillos by electroejaculation were cryopreserved in Tris diluent plus 20% egg yolk and 3% glycerol, in a fast freezing curve. Classical analysis of samples was performed after dilution, refrigeration and thawing, followed by fluorescence analysis, using a combination of fluorescent probes to assess membrane integrity (propidium iodide - PI and Hoechst - H342), and mitochondrial activity (CMXRos - Mito Tracker Red®). We also used the ultrastructural analysis to verify possible morphological alterations caused by cryoinjuries. When compared with fresh samples, we verified a significant decline in all the armadillos? semen parameters after thawing, in which only 6.1% motile sperm were found. However, the percentage of sperm which remained with viable (13%) and functional (24.7%) membranes after thawing suggests that some cells could be live but immotile. Analysis using fluorescent markers revealed that the mitochondria of armadillos? sperm is highly sensible to the freezing protocol and the findings through ultrastructure analysis proved this statement. Additionally, the images obtained by transmission electron microscopy revealed that frozenthawed sperm presented damaged plasma membrane, nuclear modifications as changes in chromatin and acrossomal changes relative to sperm capacitation. In conclusion, this study is the first attempt to cryopreserve the semen of an armadillo species, and to help us to identify critical points on the freezing-thawing procedure in order to improve the protocol. 650 $aCryopreservation 650 $aSpermatozoa 650 $aUltrastructure 650 $aCriopreservação 650 $aEspermatozoide 650 $aMembrana 650 $aMitocondria 650 $aSêmen 653 $aDano ultraestrutural e funcional 653 $aEuphractus sexcinctus 653 $aMembrane integrity 653 $aMitochondrial activity 653 $aSix-banded armadillo 653 $aSperm 653 $aTatu-peba 653 $aUltraestrutura 653 $aUltrastructural and functional damages 653 $aXenarthras 700 1 $aSANTOS, E. A. A. 700 1 $aSILVA, A. M. 700 1 $aBEZERRA, J. A. B. 700 1 $aSOUZA, A. L. P. 700 1 $aLIMA, G. L. 700 1 $aOLIVEIRA, M. F. 700 1 $aSILVA, A. R. 773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 8, p. 767-774, ago. 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
18/09/1997 |
Data da última atualização: |
06/09/2021 |
Autoria: |
REGITANO, L. C. de A.; AZEVEDO, J. L.; ROSA, A. J. M.; PACKER, I. U.; COUTINHO, L. L. |
Afiliação: |
LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE; JOÃO L. AZEVEDO, USP ESALQ; ARTUR J. M. ROSA, USP ESALQ; IRINEU U. PACKER, USP ESALQ; LUIZ L COUTINHO, USP ESALQ. |
Título: |
Evidence of selection for growth hormone, microsatellites IGF-I and CSFM50 in Canchim bovine breed. |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
Revista Brasileira de Genética, v.20, n.3, suppl., 1997, I.131, p.334. |
Idioma: |
Português |
Conteúdo: |
This study was developde to evaluate gene frequencies in different generations of Canchim cattle form the herd mantained at Centro de Pesquisa de Pecuaria do Sudeste of Brazil. |
Palavras-Chave: |
Crescimento hormonal; Growth hormone. |
Thesagro: |
Gado Canchim; Gado de Corte. |
Thesaurus NAL: |
beef cattle. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/89010/1/PROCI-1997.00086.pdf
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Marc: |
LEADER 00880naa a2200229 a 4500 001 1043879 005 2021-09-06 008 1997 bl --- 0-- u #d 100 1 $aREGITANO, L. C. de A. 245 $aEvidence of selection for growth hormone, microsatellites IGF-I and CSFM50 in Canchim bovine breed.$h[electronic resource] 260 $c1997 520 $aThis study was developde to evaluate gene frequencies in different generations of Canchim cattle form the herd mantained at Centro de Pesquisa de Pecuaria do Sudeste of Brazil. 650 $abeef cattle 650 $aGado Canchim 650 $aGado de Corte 653 $aCrescimento hormonal 653 $aGrowth hormone 700 1 $aAZEVEDO, J. L. 700 1 $aROSA, A. J. M. 700 1 $aPACKER, I. U. 700 1 $aCOUTINHO, L. L. 773 $tRevista Brasileira de Genética$gv.20, n.3, suppl., 1997, I.131, p.334.
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