Data/hora: 01/06/2024 22:19:40

Biblioteca(s): 	Embrapa Café.	
Data corrente: 	22/02/2017	
Data da última atualização: 	03/03/2017	
Autoria: 	FREITAS, R. T. da; PAIVA, R.; SALES, T. S.; SILVA, D. P. C. da; REIS, M. V. dos; SOUZA, A. C. de; ROSA, S. D. V. F. da.	
Afiliação: 	RODRIGO THEREZAN DE FREITAS, UFLA; RENATO PAIVA, UFLA; THAIS SILVA SALES, UFLA; DIOGO PEDROSA CORRÊA DA SILVA, UFLA; MICHELE VALQUÍRIA DOS REIS, UFLA; ANA CRISTINA DE SOUZA, UFLA; STTELA DELLYZETE VEIGA F DA ROSA, SAPC.	
Título: 	Cryopreservation of Coffea arabica L. zygotic embryos by vitrification.	
Ano de publicação: 	2016	
Fonte/Imprenta: 	Notulac Botanicae Horti Agrobotanici Cluj-Napoca, v. 44, n. 2, p. 445-451, Dec. 2016.	
Idioma: 	Inglês	
Conteúdo: 	As a consequence of the difficulty in conventional coffee seed storage, biotechnological alternatives such as cryopreservation have been investigated. The objective of this study was to develop a protocol for the cryopreservation of Coffea arabica L. (cv. ?Catuaí Vermelho? - IAC 144) zygotic embryos by vitrification. For the cryopreservation study, the embryos were immersed in Plant Vitrification Solution 2 at different times (0, 10, 25, 50, 100, and 250 min) and two temperatures (0 and 25 °C). Subsequently, the best thawing time was determined in a water bath (1, 3, 5 minutes or directly in Recovery Solution). An anatomical study was conducted on non-stored and stored embryos, with or without the use of Plant Vitrification Solution 2. The immersion in cryoprotectant solution for 100 min at 0 °C allows embryo cryopreservation. Embryos can be directly thawed in Recovery Solution after storage in liquid nitrogen. It was observed that Plant Vitrification Solution 2 reduced internal water content in the cells, allowing subsequent embryo growth resumption.	
Palavras-Chave: 	Liquid nitrogen; PVS2; TTC test.	
Thesaurus Nal: 	Ex situ conservation; Histology; Thawing.	
Categoria do assunto: 	--	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/156422/1/Cryopreservation-of-Coffea-arabica.pdf	
Registro original: 	Embrapa Café (CNPCa)	
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