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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
25/11/2022 |
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Data da última atualização: |
11/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VILELA, A. L. O.; ROSA, S. D. V. F. da; COELHO, S. V. B.; PEREIRA, C. C.; SOUZA, A. C. de; RIBEIRO, F. A. S. |
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Afiliação: |
ANA LUIZA OLIVEIRA VILELA, UNIVERSIDADE FEDERAL DE LAVRAS; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; STEFÂNIA VILAS BOAS COELHO, UNIVERSIDADE FEDERAL DE LAVRAS; CRISTIANE CARVALHO PEREIRA, UNIVERSIDADE FEDERAL DE LAVRAS; ANA CRISTINA DE SOUZA, UNIVERSIDADE FEDERAL DE LAVRAS; FERNANDO AUGUSTO SALES RIBEIRO, UNIVERSIDADE FEDERAL DE LAVRAS. |
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Título: |
Acclimatization of coffee seedlings obtained from zygotic embryos of aged seeds. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Australian Journal of Crop Science, v. 16, n. 9, p.112-1134, 2022. |
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Idioma: |
Inglês |
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Conteúdo: |
Coffee seeds rapidly lose viability during storage, which hinders the development of vigorous seedlings for crop establishment. There are reports that seed endosperm is more sensitive to deterioration than embryos, which can be excised and cultivated in vitro. However, a substantial number of plants grown in vitro do not survive during transfer to a greenhouse or field environment. The objective of this study was to evaluate the acclimatization of coffee seedlings of cultivar Catuaí Amarelo IAC 62, developed from zygotic embryos obtained from aged seeds in different substrates and environments, for the production of well-developed seedlings suitable for planting. For this purpose, seedlings were obtained from the in vitro cultivation of embryos obtained from seeds of two quality levels: freshly harvested seeds and artificially aged seeds. Zygotic embryos were extracted from the seeds and cultivated in MS medium. At 60 days, the percentages of normal and abnormal seedlings and dead seeds were evaluated. The good-quality seedlings grown in vitro for 60 days were transplanted into two different substrates (Tropstrato and coconut fiber) and acclimatized in two environments (growth room and greenhouse with a misting system). The plants were evaluated for height, stem diameter, number of leaves, chlorophyll content, and growth rate. The greenhouse environment was better for seedling growth, possibly due to its higher sunlight and temperature. The best substrate was coconut fiber, as it ensured better development of plants from freshly harvested seeds and those from aged seeds. It is possible to develop healthy seedlings from seeds with low viability. MenosCoffee seeds rapidly lose viability during storage, which hinders the development of vigorous seedlings for crop establishment. There are reports that seed endosperm is more sensitive to deterioration than embryos, which can be excised and cultivated in vitro. However, a substantial number of plants grown in vitro do not survive during transfer to a greenhouse or field environment. The objective of this study was to evaluate the acclimatization of coffee seedlings of cultivar Catuaí Amarelo IAC 62, developed from zygotic embryos obtained from aged seeds in different substrates and environments, for the production of well-developed seedlings suitable for planting. For this purpose, seedlings were obtained from the in vitro cultivation of embryos obtained from seeds of two quality levels: freshly harvested seeds and artificially aged seeds. Zygotic embryos were extracted from the seeds and cultivated in MS medium. At 60 days, the percentages of normal and abnormal seedlings and dead seeds were evaluated. The good-quality seedlings grown in vitro for 60 days were transplanted into two different substrates (Tropstrato and coconut fiber) and acclimatized in two environments (growth room and greenhouse with a misting system). The plants were evaluated for height, stem diameter, number of leaves, chlorophyll content, and growth rate. The greenhouse environment was better for seedling growth, possibly due to its higher sunlight and temperature. The best substrate was coconut fiber, ... Mostrar Tudo |
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Palavras-Chave: |
Zygotic embryos. |
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Thesaurus Nal: |
Coffea arabica var. arabica; Enzyme substrates; Greenhouses; In vitro culture; Seedlings; Seeds. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150877/1/Acclimatization-of-coffee-seedlings-obtained.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148763/1/Acclimatization-of-coffee-seedlings-obtained.pdf
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Marc: |
LEADER 02455naa a2200265 a 4500
001 2150877
005 2023-01-11
008 2022 bl uuuu u00u1 u #d
100 1 $aVILELA, A. L. O.
245 $aAcclimatization of coffee seedlings obtained from zygotic embryos of aged seeds.$h[electronic resource]
260 $c2022
520 $aCoffee seeds rapidly lose viability during storage, which hinders the development of vigorous seedlings for crop establishment. There are reports that seed endosperm is more sensitive to deterioration than embryos, which can be excised and cultivated in vitro. However, a substantial number of plants grown in vitro do not survive during transfer to a greenhouse or field environment. The objective of this study was to evaluate the acclimatization of coffee seedlings of cultivar Catuaí Amarelo IAC 62, developed from zygotic embryos obtained from aged seeds in different substrates and environments, for the production of well-developed seedlings suitable for planting. For this purpose, seedlings were obtained from the in vitro cultivation of embryos obtained from seeds of two quality levels: freshly harvested seeds and artificially aged seeds. Zygotic embryos were extracted from the seeds and cultivated in MS medium. At 60 days, the percentages of normal and abnormal seedlings and dead seeds were evaluated. The good-quality seedlings grown in vitro for 60 days were transplanted into two different substrates (Tropstrato and coconut fiber) and acclimatized in two environments (growth room and greenhouse with a misting system). The plants were evaluated for height, stem diameter, number of leaves, chlorophyll content, and growth rate. The greenhouse environment was better for seedling growth, possibly due to its higher sunlight and temperature. The best substrate was coconut fiber, as it ensured better development of plants from freshly harvested seeds and those from aged seeds. It is possible to develop healthy seedlings from seeds with low viability.
650 $aCoffea arabica var. arabica
650 $aEnzyme substrates
650 $aGreenhouses
650 $aIn vitro culture
650 $aSeedlings
650 $aSeeds
653 $aZygotic embryos
700 1 $aROSA, S. D. V. F. da
700 1 $aCOELHO, S. V. B.
700 1 $aPEREIRA, C. C.
700 1 $aSOUZA, A. C. de
700 1 $aRIBEIRO, F. A. S.
773 $tAustralian Journal of Crop Science$gv. 16, n. 9, p.112-1134, 2022.
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Registro original: |
Embrapa Café (CNPCa) |
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Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
28/12/2020 |
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Data da última atualização: |
28/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
SOUZA, L. N. B. de; DIAS, N. D. S. C.; SANTANA, V. de O.; SILVEIRA, L. A.; MEIRA, M. R.; SANTOS, E. S. L. dos; FALEIRO, F. G.; CERQUEIRA-SILVA, C. B. M. |
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Afiliação: |
FABIO GELAPE FALEIRO, CPAC. |
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Título: |
Amplification test and selection of markers analogue to resistance genes in species and commercial varieties of Passiflora spp. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Multi-Science Journal, v. 3, n. 1, 2020. |
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Páginas: |
p. 61-75 |
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Idioma: |
Português |
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Conteúdo: |
BSTRACT. Brazil is highlighted as the largest producer and consumer of passion fruit (Passiflora) and holder of representative biodiversity. Studies dedicated to the genetic characterization of the genus in order to understand the existing diversity are important for the advancement of genetic improvement and conservation programs. The aim of this study was to select combinations of RGA (Resistance Genes Analogs) primers for commercial passion fruit species and varieties. To this end, amplification tests were performed on a PCR platform in 17 combinations of RGA primers for eight species and 12 commercial varieties of passion fruit from the Banco Ativo de Germoplasma Flor da Paixão. All combinations of primers have generated amplification products in at least one of the Passiflora samples. The number of combinations that generated amplification products varied from seven to 12 in the species and from three to eight in the varieties. Three to 12 combinations generated amplification products for the samples, emphasizing that the RGA markers were efficient in accessing genomic loci in the species and varieties of passion fruit. The variation of efficient combinations in each of the species and varieties attested to the importance of this research in the preliminary stages of genetic studies as auxiliary tools in conservation and genetic improvement programs. However, this study made it possible to identify combinations of primers to be prioritized in inter and intraspecific genetic characterizations of passion fruit MenosBSTRACT. Brazil is highlighted as the largest producer and consumer of passion fruit (Passiflora) and holder of representative biodiversity. Studies dedicated to the genetic characterization of the genus in order to understand the existing diversity are important for the advancement of genetic improvement and conservation programs. The aim of this study was to select combinations of RGA (Resistance Genes Analogs) primers for commercial passion fruit species and varieties. To this end, amplification tests were performed on a PCR platform in 17 combinations of RGA primers for eight species and 12 commercial varieties of passion fruit from the Banco Ativo de Germoplasma Flor da Paixão. All combinations of primers have generated amplification products in at least one of the Passiflora samples. The number of combinations that generated amplification products varied from seven to 12 in the species and from three to eight in the varieties. Three to 12 combinations generated amplification products for the samples, emphasizing that the RGA markers were efficient in accessing genomic loci in the species and varieties of passion fruit. The variation of efficient combinations in each of the species and varieties attested to the importance of this research in the preliminary stages of genetic studies as auxiliary tools in conservation and genetic improvement programs. However, this study made it possible to identify combinations of primers to be prioritized in inter and intraspecific gen... Mostrar Tudo |
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Thesagro: |
Banco de Germoplasma; Passifloraceae; Seleção Genética. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219730/1/FABIO-FALEIRO-Amplification-test-and-selection-of-markers-analog.pdf
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Marc: |
LEADER 02326naa a2200253 a 4500
001 2128776
005 2020-12-28
008 2020 bl uuuu u00u1 u #d
100 1 $aSOUZA, L. N. B. de
245 $aAmplification test and selection of markers analogue to resistance genes in species and commercial varieties of Passiflora spp.$h[electronic resource]
260 $c2020
300 $ap. 61-75
520 $aBSTRACT. Brazil is highlighted as the largest producer and consumer of passion fruit (Passiflora) and holder of representative biodiversity. Studies dedicated to the genetic characterization of the genus in order to understand the existing diversity are important for the advancement of genetic improvement and conservation programs. The aim of this study was to select combinations of RGA (Resistance Genes Analogs) primers for commercial passion fruit species and varieties. To this end, amplification tests were performed on a PCR platform in 17 combinations of RGA primers for eight species and 12 commercial varieties of passion fruit from the Banco Ativo de Germoplasma Flor da Paixão. All combinations of primers have generated amplification products in at least one of the Passiflora samples. The number of combinations that generated amplification products varied from seven to 12 in the species and from three to eight in the varieties. Three to 12 combinations generated amplification products for the samples, emphasizing that the RGA markers were efficient in accessing genomic loci in the species and varieties of passion fruit. The variation of efficient combinations in each of the species and varieties attested to the importance of this research in the preliminary stages of genetic studies as auxiliary tools in conservation and genetic improvement programs. However, this study made it possible to identify combinations of primers to be prioritized in inter and intraspecific genetic characterizations of passion fruit
650 $aBanco de Germoplasma
650 $aPassifloraceae
650 $aSeleção Genética
700 1 $aDIAS, N. D. S. C.
700 1 $aSANTANA, V. de O.
700 1 $aSILVEIRA, L. A.
700 1 $aMEIRA, M. R.
700 1 $aSANTOS, E. S. L. dos
700 1 $aFALEIRO, F. G.
700 1 $aCERQUEIRA-SILVA, C. B. M.
773 $tMulti-Science Journal$gv. 3, n. 1, 2020.
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Embrapa Cerrados (CPAC) |
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