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| 1. |  | MARTINS, D. M.; CLEMENTINO, C. de S.; SOUSA, S. de A.; BARBOSA, F. J. V.; DINIZ, F. M. Fatores climáticos intervenientes na incubação artificial de ovos de galinhas caipiras (Gallus gallus domesticus). In: CONGRESSO BRASILEIRO DE ORNITOLOGIA, 16., 2008, Palmas,TO. A ornitologia no Cerrado e Ecótonos do Brasil: livro de resumos. Palmas,TO: CBO: Universidade Federal do Tocantins: Sociedade Brasileira de Ornitologia: Grupo de Pesquisa em Ecologia e Conservação de Aves, 2008. p. 48| Biblioteca(s): Embrapa Meio-Norte. |
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| 2. | | LAVELLE, P.; DOLEDEC, S.; GOND, V.; OSZWALD, J.; VEIGA, I.; RAMIREZ, B.; SANTOS, W.; SARTRE, A. de; DECAENS, T.; GRIMALDI, M.; HUBERT, B.; MARTINS, M.; LENA, P.; ROBERT, P. de; FEIJOO, A.; HURTADO, M. del P.; RODRIGUEZ, G.; MITJA, D.; MIRANDA, I.; GORDILLO, E.; VELASQUEZ, E.; OTERO, T.; VELASQUEZ, A.; THIOLLAY, J. M.; SOUSA, S. de; MORENO, L. E.; BROWN, G. G.; MARICHAL, R.; CHACON, P.; SANABRIA, C.; DESJARDINS, T.; SANTANA LIMA, T.; MICHELOTTI, F.; VILLANUEVA, O.; VELASQUEZ, J.; GUEVARRA, E.; FONTE, E. The need for eco-efficient landscapes to prevent irreversible degradation of agroecosystems in deforested Amazonia. In: TROPENTAG 2012: INTERNATIONAL RESEARCH ON FOOD SECURITY, NATURAL RESOURCE MANAGEMENT AND RURAL DEVELOPMENT, 2012, Göttingen. Resilience of agricultural systems against crises: book of abstracts. Göttingen: Cuvillier Verlag, 2012. Disponível online.| Biblioteca(s): Embrapa Florestas. |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
02/08/2018 |
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Data da última atualização: |
02/08/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
RAMOS, C. A. N.; ARAÚJO, F. R.; SOUZA, I. I. F.; OLIVEIRA, R. H. M. de; ELISEI, C.; SOARES, C. O.; SACCO, A. M. S.; ROSINHA, G. M. S.; ALVES, L. C. |
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Afiliação: |
CARLOS ALBERTO DO NASCIMENTO RAMOS, UFRPE; FLABIO RIBEIRO ARAUJO, CNPGC; INGRID IEDA FERNANDO DE SOUZA, BOLSISTA - CNPGC; RENATO HENRIQUE MARCAL DE OLIVEIRA, CNPGC; CARINA ELISEI, BOLSISTA - CNPGC; CLEBER OLIVEIRA SOARES, CNPGC; ANA MARIA SASTRE SACCO, CPPSUL; GRACIA MARIA SOARES ROSINHA, CNPGC; LEUCIO C. ALVES, UFRPE. |
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Título: |
Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n. 7, p. 998-1002, nov. 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
Babesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovis as well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis. The aims of the present paper were to analyse the genetic conservation of the P0 gene among Brazilian isolates of B. bovis and produce recombinant P0 (rPO) from a Brazilian isolate and evaluate its antigenicity with sera from cattle naturally or experimentally infected with B. bovis and sera from cattle experimentally infected with B. bigemina in an enzyme-linked immunosorbent assay (ELISA). MenosBabesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovis as well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis. The aims of the present paper were to analyse the genetic conservation of the P0 gene among Brazilian isolates of B. bovis and produce recombinant P0 (rPO) from a Brazilian isolate and evaluate its antigenicity with sera from cattle naturally or experimentally infected with B. bovis and sera from cattle experimentally infected with B. bige... Mostrar Tudo |
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Palavras-Chave: |
Babesiose bovina. |
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Thesagro: |
Babesia bovis; Bovino; Sanidade animal. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/180825/1/Ramos-2009-Molecular-and-antigenic-characteris.pdf
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Marc: |
LEADER 02353naa a2200265 a 4500
001 2093859
005 2018-08-02
008 2009 bl uuuu u00u1 u #d
100 1 $aRAMOS, C. A. N.
245 $aMolecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis.$h[electronic resource]
260 $c2009
520 $aBabesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovis as well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis. The aims of the present paper were to analyse the genetic conservation of the P0 gene among Brazilian isolates of B. bovis and produce recombinant P0 (rPO) from a Brazilian isolate and evaluate its antigenicity with sera from cattle naturally or experimentally infected with B. bovis and sera from cattle experimentally infected with B. bigemina in an enzyme-linked immunosorbent assay (ELISA).
650 $aBabesia bovis
650 $aBovino
650 $aSanidade animal
653 $aBabesiose bovina
700 1 $aARAÚJO, F. R.
700 1 $aSOUZA, I. I. F.
700 1 $aOLIVEIRA, R. H. M. de
700 1 $aELISEI, C.
700 1 $aSOARES, C. O.
700 1 $aSACCO, A. M. S.
700 1 $aROSINHA, G. M. S.
700 1 $aALVES, L. C.
773 $tMemórias do Instituto Oswaldo Cruz, Rio de Janeiro$gv. 104, n. 7, p. 998-1002, nov. 2009.
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Embrapa Pecuária Sul (CPPSUL) |
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