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Registro Completo |
Biblioteca(s): |
Embrapa Agrossilvipastoril. |
Data corrente: |
06/02/2018 |
Data da última atualização: |
06/02/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SOUSA, N. L.; CABRAL, G. B.; VIEIRA, P. M.; BALDONI, A. B.; ARAGAO, F. J. L. |
Afiliação: |
NATÁLIA L. SOUSA, UNB; GLAUCIA BARBOSA CABRAL, Cenargen; PABLINE M. VIEIRA, INSTITUTO FEDERAL GOIANO; AISY BOTEGA BALDONI TARDIN, CPAMT; FRANCISCO JOSE LIMA ARAGAO, Cenargen. |
Título: |
Bio-detoxification of ricin in castor bean (Ricinus communis L.) seeds. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Scientific Reports, v. .7, n. 1, 2017. (Open). |
DOI: |
10.1038/s41598-017-15636-7 |
Idioma: |
Inglês |
Conteúdo: |
Ricin is a highly toxic ribosome-inactivating lectin occurring in the seeds of castor bean (Ricinus communis L.). Castor bean grows throughout tropical and sub-tropical regions and is a very important crop due to its high seed content of ricinoleic acid, an unusual fatty acid, which has several industrial applications. However, due to the presence of the toxin, castor bean can cause death after the exposure of animals to low doses of ricin through skin contact, injection, inhalation or oral routes. Aiming to generate a detoxified genotype, we explored the RNAi concept in order to silence the ricin coding genes in the endosperm of castor bean seeds. Results indicated that ricin genes were effectively silenced in genetically modified (GM) plants, and ricin proteins were not detected by ELISA. Hemagglutination activity was not observed with proteins isolated from GM seeds. In addition, we demonstrated that seed proteins from GM plants were not toxic to rat intestine epithelial cells or to Swiss Webster mice. After oil extraction, bio-detoxified castor bean cake, which is very rich in valuable proteins, can be used for animal feeding. Gene silencing would make castor bean cultivation safer for farmers, industrial workers and society. |
Palavras-Chave: |
Toxic ribosome-inactivating. |
Categoria do assunto: |
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Marc: |
LEADER 01828naa a2200193 a 4500 001 2087235 005 2018-02-06 008 2017 bl --- 0-- u #d 024 7 $a10.1038/s41598-017-15636-7$2DOI 100 1 $aSOUSA, N. L. 245 $aBio-detoxification of ricin in castor bean (Ricinus communis L.) seeds.$h[electronic resource] 260 $c2017 520 $aRicin is a highly toxic ribosome-inactivating lectin occurring in the seeds of castor bean (Ricinus communis L.). Castor bean grows throughout tropical and sub-tropical regions and is a very important crop due to its high seed content of ricinoleic acid, an unusual fatty acid, which has several industrial applications. However, due to the presence of the toxin, castor bean can cause death after the exposure of animals to low doses of ricin through skin contact, injection, inhalation or oral routes. Aiming to generate a detoxified genotype, we explored the RNAi concept in order to silence the ricin coding genes in the endosperm of castor bean seeds. Results indicated that ricin genes were effectively silenced in genetically modified (GM) plants, and ricin proteins were not detected by ELISA. Hemagglutination activity was not observed with proteins isolated from GM seeds. In addition, we demonstrated that seed proteins from GM plants were not toxic to rat intestine epithelial cells or to Swiss Webster mice. After oil extraction, bio-detoxified castor bean cake, which is very rich in valuable proteins, can be used for animal feeding. Gene silencing would make castor bean cultivation safer for farmers, industrial workers and society. 653 $aToxic ribosome-inactivating 700 1 $aCABRAL, G. B. 700 1 $aVIEIRA, P. M. 700 1 $aBALDONI, A. B. 700 1 $aARAGAO, F. J. L. 773 $tScientific Reports$gv. .7, n. 1, 2017. (Open).
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Embrapa Agrossilvipastoril (CPAMT) |
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Registro Completo
Biblioteca(s): |
Embrapa Agroindústria Tropical. |
Data corrente: |
28/02/2013 |
Data da última atualização: |
25/04/2013 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
LIMA, J. R. de; GONÇALVES, L. R. B.; BRANDÃO, L. R.; ROSA, C. R.; VIANA, F. M. P. |
Afiliação: |
Jaqueline Rabelo de Lima, RENORBIO; Luciana Rocha Barros Gonçalves, Derpartamento Engenharia Quimica-Universiade Federal do Ceará; Luciana Rocha Brandão, Derpartamento de Microbiologia ,ICB, Universidade Federal; Carlos Augusto Rosa, Departamento de Microbiologia, ICB, Universidades Federal de Minas Gerais; FRANCISCO MARTO PINTO VIANA, CNPAT. |
Título: |
Isolation, identification and activity in vitroof killer yeasts against Colletotrichum gloeosporioides isolated from tropical fruits. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Journal of Basic Microbiology, v. 52, p. 1-10, 2012. |
Idioma: |
Português |
Palavras-Chave: |
Activity; Against; Gloeosporioides; Identification; Isolated; Isolation; Killer; Tropical fruits; Vitroof. |
Thesaurus NAL: |
Colletotrichum; yeasts. |
Categoria do assunto: |
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Marc: |
LEADER 00818naa a2200289 a 4500 001 1951636 005 2013-04-25 008 2012 bl uuuu u00u1 u #d 100 1 $aLIMA, J. R. de 245 $aIsolation, identification and activity in vitroof killer yeasts against Colletotrichum gloeosporioides isolated from tropical fruits. 260 $c2012 650 $aColletotrichum 650 $ayeasts 653 $aActivity 653 $aAgainst 653 $aGloeosporioides 653 $aIdentification 653 $aIsolated 653 $aIsolation 653 $aKiller 653 $aTropical fruits 653 $aVitroof 700 1 $aGONÇALVES, L. R. B. 700 1 $aBRANDÃO, L. R. 700 1 $aROSA, C. R. 700 1 $aVIANA, F. M. P. 773 $tJournal of Basic Microbiology$gv. 52, p. 1-10, 2012.
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