Data/hora: 16/06/2024 07:46:38

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 30/04/2018
Data da última atualização: | 30/04/2018
Autoria: | NOGUEIRA, S. S. S.; SOUSA, M. G.; GAVA, F. N.; ROSA, F. A.; MELO, G. D.; DITTRICH, G.; MACHADO, G. F.; CAMACHO, A. A.
Afiliação: | Sheila S. S. Nogueira, Departamento de Clínica e Cirurgia Veterinária/Universidade Estadual Paulista - Unesp; Marlos G. Sousa, Departamento de Medicina Veterinária/Universidade Federal do Paraná - UFPR; Fabio N. Gava, Departamento de Clínica e Cirurgia Veterinária/Universidade Estadual Paulista - Unesp; Fernando A. Rosa, Departamento de Clínica e Cirurgia Veterinária/Universidade Estadual Paulista - Unesp; Guilherme D. Melo, Departamento de Clínica/Cirurgia e Reprodução Animal/Unesp; Gustavo Dittrich, Departamento de Medicina Veterinária/Universidade Federal do Paraná - UFPR; Gisele F. Machado, Departamento de Clínica/Cirurgia e Reprodução Animal/Unesp; Aparecido A. Camacho, Departamento de Clínica e Cirurgia Veterinária/Universidade Estadual Paulista - Unesp.
Título: | Matrix metalloproteinases 2 and 9 in rabbits with doxorubicin-induced cardiomyopathy.
Ano de publicação: | 2018
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 2, p. 320-327, fevereiro 2018
Idioma: | Inglês
Notas: | Título em português: Metaloproteinases de matriz 2 e 9 em coelhos com cardiomiopatia induzida pela doxorrubicina.
Conteúdo: | Some studies have shown the role played by matrix metalloproteinases and their inhibitors in doxorubicin cardiotoxicity. In this study, we sought to investigate how plasma and myocardial MMP 2 and 9 perform in rabbits with doxorubicin-induced cardiomyopathy, searching for a correlation between the activity of these collagenases and cardiac remodeling. Cardiomyopathy was induced by doxorubicin given intravenously twice a week for six consecutive weeks. Plasma MMP activity and the echocardiogram were assessed at baseline, and at 15 and 45 days after first injection of doxorubicin. The myocardial activity of these enzymes was solely evaluated in nine rabbits at 45 days, and results were compared with nine healthy controls. We only identified the full-length forms of both MMP 2 and 9 throughout the study. The plasma pro-MMP 2 reduced along the deterioration of cardiac function, while the pro-MMP 9 increased significantly at T45 as compared to baseline and T15. A negative significant correlation was found to exist between the plasma activity of pro-MMP 2 and mitral E-to-mitral septal annular early diastolic velocity ratio, which is an estimate of mean left atrial pressure and congestion. Only pro-MMP 2 was found in myocardial samples,and mean activity of such enzyme was statistically lower than that recorded for healthy controls. Although no active form was documented for either collagenase, the duration of the treatment with doxorubicin played a role in the alteration of plasma pro-forms activity. However, these changes could not be associated with most echocardiographic parameters that are supportive of cardiac remodeling.
Palavras-Chave: | Cardiomiopatia; Doxorrubicina; Echocardiogram; Ecocardio; MetaloproteinaseS; Zimografia; Zymography.
Thesagro: | Atividade Enzimática; Cardiopatia; Coelho.
Thesaurus Nal: | Cardiomyopathy; Collagenase; Doxorubicin; Enzyme activity; Heart diseases; Metalloproteinases; Rabbits.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/176152/1/Matrix-metalloproteinases-2-and-9-in-rabbits.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (62562-1) - UPE - AP - DD -  -  -  - DIS