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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
07/01/2011 |
Data da última atualização: |
29/05/2018 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SANTOS, C. V.; CRUZ, I.; SILVA, I. F. da; SILVA, R. B. da; CASTRO, A. L. G. de; FIGUEIREDO, M. de L. C.; SOUSA, L. P. S. P. de; FIGUEIREDO, R. de J. |
Afiliação: |
CAMILA VIEIRA SANTOS, Bolsista; IVAN CRUZ, CNPMS; IVANA FERNANDES DA SILVA, Bolsista; RAFAEL BRAGA DA SILVA, Bolsista; ANA LUISA GANGANA DE CASTRO, Bolsista; MARIA DE LOURDES CORRÊA FIGUEIREDO, Bolsista; LUANA PATRÍCIA SANTANA PEREIRA DE SOUSA, Bolsista; ROBERTA DE JESUS FIGUEIREDO, Bolsista. |
Título: |
Aspectos biológicos de Spodoptera (J. E. Smith) (Lepidoptera: noctuidae) em diferentes temperaturas. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Português |
Thesagro: |
Lagarta; Praga de planta. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/25004/1/CBE-16.pdf
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Marc: |
LEADER 00748nam a2200217 a 4500 001 1872348 005 2018-05-29 008 2010 bl uuuu u00u1 u #d 100 1 $aSANTOS, C. V. 245 $aAspectos biológicos de Spodoptera (J. E. Smith) (Lepidoptera$bnoctuidae) em diferentes temperaturas.$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Anais... Natal: Sociedade Brasileira de Entomologia$c2010 300 $c1 CD-ROM. 650 $aLagarta 650 $aPraga de planta 700 1 $aCRUZ, I. 700 1 $aSILVA, I. F. da 700 1 $aSILVA, R. B. da 700 1 $aCASTRO, A. L. G. de 700 1 $aFIGUEIREDO, M. de L. C. 700 1 $aSOUSA, L. P. S. P. de 700 1 $aFIGUEIREDO, R. de J.
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Embrapa Milho e Sorgo (CNPMS) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
05/06/2020 |
Data da última atualização: |
16/08/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
SOARES, A. C.; SOARES, J. C.; PASCHOALIN, R. T.; RODRIGUES, V. C.; MELENDEZ, M. E.; REIS, R. M.; CARVALHO, A. L.; MATTOSO, L. H. C.; OLIVEIRA, O. N. |
Afiliação: |
LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA. |
Título: |
Immunosensors containing solution blow spun fibers of poly(lactic acid) to detect p53 biomarker. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Materials Science & Engineering C, v. 115, 2020. |
Páginas: |
1-10 |
ISSN: |
0928-4931 |
DOI: |
https://doi.org/10.1016/j.msec.2020.111120 |
Idioma: |
Inglês |
Conteúdo: |
This paper reports on biosensors made with a matrix of polylactic acid (PLA) fibers, which are suitable for immobilization of the anti-p53 active layer for detection of p53 biomarker. The PLA fibers were produced with solution blow spinning, a method that is advantageous for its simplicity and possibility to tune the fiber properties. For the biosensors, the optimized time to deposit the fibers was 60 s, with which detection of p53 could be achieved with the limit of detection of 11 pg/mL using electrical impedance spectroscopy. This sensitivity is also sufficient to detect the p53 biomarker in patient samples, which was confirmed by distinguishing samples from cell lines with distinct p53 concentrations in a plot where the impedance spectra were visualized with the interactive document mapping (IDMAP) technique. The high sensitivity and selectivity of the biosensors may be attributed to the specific interaction between the active layer and p53 modeled with a Langmuir-Freundlich and Freundlich isotherms and inferred from the analysis of the vibrational bands at 1550, 1650 and 1757 cm−1 using polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). The successful immobilization of the active layer is evidence that the approach based on solution blown spun fibers may be replicated to other types of biosensors. |
Palavras-Chave: |
Immunosensor; PLA fibers; Solution blow spinning. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02209naa a2200289 a 4500 001 2123069 005 2022-08-16 008 2020 bl uuuu u00u1 u #d 022 $a0928-4931 024 7 $ahttps://doi.org/10.1016/j.msec.2020.111120$2DOI 100 1 $aSOARES, A. C. 245 $aImmunosensors containing solution blow spun fibers of poly(lactic acid) to detect p53 biomarker.$h[electronic resource] 260 $c2020 300 $a1-10 520 $aThis paper reports on biosensors made with a matrix of polylactic acid (PLA) fibers, which are suitable for immobilization of the anti-p53 active layer for detection of p53 biomarker. The PLA fibers were produced with solution blow spinning, a method that is advantageous for its simplicity and possibility to tune the fiber properties. For the biosensors, the optimized time to deposit the fibers was 60 s, with which detection of p53 could be achieved with the limit of detection of 11 pg/mL using electrical impedance spectroscopy. This sensitivity is also sufficient to detect the p53 biomarker in patient samples, which was confirmed by distinguishing samples from cell lines with distinct p53 concentrations in a plot where the impedance spectra were visualized with the interactive document mapping (IDMAP) technique. The high sensitivity and selectivity of the biosensors may be attributed to the specific interaction between the active layer and p53 modeled with a Langmuir-Freundlich and Freundlich isotherms and inferred from the analysis of the vibrational bands at 1550, 1650 and 1757 cm−1 using polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). The successful immobilization of the active layer is evidence that the approach based on solution blown spun fibers may be replicated to other types of biosensors. 653 $aImmunosensor 653 $aPLA fibers 653 $aSolution blow spinning 700 1 $aSOARES, J. C. 700 1 $aPASCHOALIN, R. T. 700 1 $aRODRIGUES, V. C. 700 1 $aMELENDEZ, M. E. 700 1 $aREIS, R. M. 700 1 $aCARVALHO, A. L. 700 1 $aMATTOSO, L. H. C. 700 1 $aOLIVEIRA, O. N. 773 $tMaterials Science & Engineering C$gv. 115, 2020.
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