Data/hora: 23/09/2024 15:44:23

Biblioteca(s): 	Embrapa Agroenergia; Embrapa Meio-Norte.	
Data corrente: 	26/01/2022	
Data da última atualização: 	21/02/2022	
Autoria: 	SILVA, T. L. C. da; SILVA, V. N. B.; BRAGA, I. de O.; RODRIGUES NETO, J. C.; LEAO, A. P.; RIBEIRO, J. A. de A.; VALADARES, L. F.; ABDELNUR, P. V.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T.	
Afiliação: 	THALLITON LUIZ CARVALHO DA SILVA, Programa de Pós-Graduação em Biotecnologia Vegetal, Universidade Federal de Lavras, MG, Brasil.; VIVIANNY NAYSE BELO SILVA, Programa de Pós-Graduação em Biotecnologia Vegetal, Universidade Federal de Lavras, MG, Brasil.; ÍTALO DE OLIVEIRA BRAGA, Programa de Pós-Graduação em Biotecnologia Vegetal, Universidade Federal de Lavras, MG, Brasil.; JORGE CANDIDO RODRIGUES NETO, Instituto de Química, Universidade Federal de Goiás, Goiânia, GO, Brasil.; ANDRE PEREIRA LEAO, CNPAE; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; LEONARDO FONSECA VALADARES, CNPAE; PATRICIA VERARDI ABDELNUR, CNPAE; CARLOS ANTONIO FERREIRA DE SOUSA, CPAMN; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE.	
Título: 	Integration of metabolomics and transcriptomics data to further characterize Gliricidia sepium (Jacq.) Kunth under high salinity stress.	
Ano de publicação: 	2021	
Fonte/Imprenta: 	Plant Genome, e20182, 2021.	
Idioma: 	Inglês	
Conteúdo: 	Soil salinity is one abiotic stress that threatens agriculture in more than 100 countries. Gliricidia [Gliricidia sepium (Jacq.) Kunth] is a multipurpose tree known for its ability to adapt to a wide range of soils; however, its tolerance limits and responses to salt stress are not yet well understood. In this study, after characterizing the morphophysiological responses of young gliricidia plants to salinity stress, leaf metabolic and transcription profiles were generated and submitted to single and integrated analyses. RNA from leaf samples were subjected to RNA sequencing using an Illumina HiSeq platform and the paired-end strategy. Polar and lipidic fractions from leaf samples were extracted and analyzed on an ultra-high-performance liquid chromatography (UHPLC) coupled with electrospray ionization quadrupole time-offlight high-resolution mass spectrometry (MS) system. Acquired data were analyzed using the OmicsBox, XCMS Online, MetaboAnalyst, and Omics Fusion platforms. The substrate salinization protocol used allowed the identification of two distinct responses to salt stress: tolerance and adaptation. Single analysis on transcriptome and metabolome data sets led to a group of 5,672 transcripts and 107 metabolites differentially expressed in gliricidia leaves under salt stress. The phenylpropanoid biosynthesis was the most affected pathway, with 15 metabolites and three genes differentially expressed. Results showed that the differentially expressed metabolites and genes from this pathway affect mainly short-term salt stress (STS). The single analysis of the transcriptome identified 12 genes coding for proteins that might play a role in gliricidia response at both STS and long-termsalt stress (LTS). Further studies are needed to reveal the mechanisms behind the adaptation response.	
Palavras-Chave: 	Adaptation; Salinization protocol.	
Categoria do assunto: 	--	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/230514/1/The-Plant-Genome-2022-Integration.pdf	
Registro original: 	Embrapa Agroenergia (CNPAE)	
Exemplares: 	CNPAE - (4010-1) - UPC - AP - DD -  -  -  - DIS
CPAMN - (33251-1) - UPC - AP - DD -  -  -  - DIS