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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
27/10/2015 |
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Data da última atualização: |
18/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DÓRIA, M. S.; SOUSA, A. O. de; BARBOSA, C. de J.; COSTA, M. G. C.; GESTEIRA, A. da S.; SOUZA, R. M.; FREITAS, A. C. O.; PIROVANI, C. P. |
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Afiliação: |
MILENA SANTOS DÓRIA, UESC; AURIZÂNGELA OLIVEIRA DE SOUSA, UESC; CRISTIANE DE JESUS BARBOSA, CNPMF; MÁRCIO GILBERTO CARDOSO COSTA, UESC; ABELMON DA SILVA GESTEIRA, CNPMF; REGINA MARTINS SOUZA; ANA CAMILA OLIVEIRA FREITAS, UESC; CARLOS PRIMINHO PIROVANI, UESC. |
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Título: |
Citrus tristeza virus (CTV) causing proteomic and enzymatic changes in sweet orange variety "Westin". |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plos One, July, 2015. |
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ISSN: |
1932-6203 |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus Tristeza disease, caused by CTV (Citrus tristeza virus), committs citrus plantations around the world and specifically attacks phloem tissues of the plant. The virus exists as a mixture of more or less severe variants, which may or may not cause symptoms of Tristeza. The objective of this study was to analyze the changes caused by CTV in the proteome of stems of sweet orange, as well as in the activity and gene expression of antioxidant enzymes. The CTV-infected sweet orange displayed mild symptoms, which were characterized by the presence of sparse stem pitting throughout their stems. The presence of virus was confirmed by RT-PCR. Proteomic analysis by 2DE-PAGE-MS / MS revealed the identity of 40 proteins differentially expressed between CTV- infected and -non-infected samples. Of these, 33 were up-regulated and 7 were down-regulated in CTV-infected samples. Among the proteins identified stands out a specific from the virus, the coat protein. Other proteins identified are involved with oxidative stress and for this their enzymatic activity was measured. The activity of superoxide dismutase (SOD) was higher in CTV-infected samples, as catalase (CAT) showed higher activity in uninfected samples. The activity of guaiacol peroxidase (GPX) did not vary significantly between samples. However, ascorbate peroxidase (APX) was more active in the infected samples. The relative expression of the genes encoding CAT, SOD, APX and GPX was analyzed by quantitative real time PCR (RTqPCR). The CTV-infected samples showed greater accumulation of transcripts, except for the CAT gene. This gene showed higher expression in the uninfected samples. Taken together, it can be concluded that the CTV affects the protein profile and activity and gene expression of antioxidant enzymes in plants infected by this virus. MenosCitrus Tristeza disease, caused by CTV (Citrus tristeza virus), committs citrus plantations around the world and specifically attacks phloem tissues of the plant. The virus exists as a mixture of more or less severe variants, which may or may not cause symptoms of Tristeza. The objective of this study was to analyze the changes caused by CTV in the proteome of stems of sweet orange, as well as in the activity and gene expression of antioxidant enzymes. The CTV-infected sweet orange displayed mild symptoms, which were characterized by the presence of sparse stem pitting throughout their stems. The presence of virus was confirmed by RT-PCR. Proteomic analysis by 2DE-PAGE-MS / MS revealed the identity of 40 proteins differentially expressed between CTV- infected and -non-infected samples. Of these, 33 were up-regulated and 7 were down-regulated in CTV-infected samples. Among the proteins identified stands out a specific from the virus, the coat protein. Other proteins identified are involved with oxidative stress and for this their enzymatic activity was measured. The activity of superoxide dismutase (SOD) was higher in CTV-infected samples, as catalase (CAT) showed higher activity in uninfected samples. The activity of guaiacol peroxidase (GPX) did not vary significantly between samples. However, ascorbate peroxidase (APX) was more active in the infected samples. The relative expression of the genes encoding CAT, SOD, APX and GPX was analyzed by quantitative real time PCR (RTq... Mostrar Tudo |
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Palavras-Chave: |
Plant disease. |
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Thesagro: |
Doença de planta; Fruta cítrica; Vírus. |
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Thesaurus Nal: |
Citrus. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02591naa a2200277 a 4500
001 2027385
005 2023-05-18
008 2015 bl uuuu u00u1 u #d
022 $a1932-6203
100 1 $aDÓRIA, M. S.
245 $aCitrus tristeza virus (CTV) causing proteomic and enzymatic changes in sweet orange variety "Westin".$h[electronic resource]
260 $c2015
520 $aCitrus Tristeza disease, caused by CTV (Citrus tristeza virus), committs citrus plantations around the world and specifically attacks phloem tissues of the plant. The virus exists as a mixture of more or less severe variants, which may or may not cause symptoms of Tristeza. The objective of this study was to analyze the changes caused by CTV in the proteome of stems of sweet orange, as well as in the activity and gene expression of antioxidant enzymes. The CTV-infected sweet orange displayed mild symptoms, which were characterized by the presence of sparse stem pitting throughout their stems. The presence of virus was confirmed by RT-PCR. Proteomic analysis by 2DE-PAGE-MS / MS revealed the identity of 40 proteins differentially expressed between CTV- infected and -non-infected samples. Of these, 33 were up-regulated and 7 were down-regulated in CTV-infected samples. Among the proteins identified stands out a specific from the virus, the coat protein. Other proteins identified are involved with oxidative stress and for this their enzymatic activity was measured. The activity of superoxide dismutase (SOD) was higher in CTV-infected samples, as catalase (CAT) showed higher activity in uninfected samples. The activity of guaiacol peroxidase (GPX) did not vary significantly between samples. However, ascorbate peroxidase (APX) was more active in the infected samples. The relative expression of the genes encoding CAT, SOD, APX and GPX was analyzed by quantitative real time PCR (RTqPCR). The CTV-infected samples showed greater accumulation of transcripts, except for the CAT gene. This gene showed higher expression in the uninfected samples. Taken together, it can be concluded that the CTV affects the protein profile and activity and gene expression of antioxidant enzymes in plants infected by this virus.
650 $aCitrus
650 $aDoença de planta
650 $aFruta cítrica
650 $aVírus
653 $aPlant disease
700 1 $aSOUSA, A. O. de
700 1 $aBARBOSA, C. de J.
700 1 $aCOSTA, M. G. C.
700 1 $aGESTEIRA, A. da S.
700 1 $aSOUZA, R. M.
700 1 $aFREITAS, A. C. O.
700 1 $aPIROVANI, C. P.
773 $tPlos One, July, 2015.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 13 | |
| 1. |  | FRANÇA, A. M. S.; SANO, E. E.; SOUSA, A. O. Avaliação de fusão de imagens CBERS/2B no mapeamento de áreas úmidas do Distrito Federal. In: ENCONTRO DE JOVENS TALENTOS DA EMBRAPA CERRADOS, 4., 2009, Planaltina, DF. Resumos apresentados... Planaltina, DF: Embrapa Cerrados, 2009. p. 155-156 (Embrapa Cerrados. Documentos, 243).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 4. | | SOUSA, A. O.; RODRIGUES, B. A.; NARCISO, M. G.; MENESES, M. V.; GUIMARAES, C. M. Software de obtenção do Índice de Vegetação por Diferença Normalizada. In: SEMINÁRIO JOVENS TALENTOS, 9., 2015, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2015. p. 94. (Embrapa Arroz e Feijão. Documentos, 309).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 5. | | ORNELLAS, F. L. S.; DANTAS, B. F.; COSTA, D. S.; SOUSA, A. O.; BARBOSA, R. M. Teste de vigor baseado na liberação de etanol em sementes de melão. Informativo ABRATES, Londrina, v. 27, n. 2, p. 204, ago. 2017. Número especial. Edição dos Resumos do XX Congresso Brasileiro de Sementes, Foz do Iguaçu, ago. 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 6. | | ALMEIDA, A. P.; ANDRIOLI, A.; SOUSA, A. O.; MACIEL, T. T. Superovulação em caprinos nativos da raça Moxotó. In: CONGRESSO BRASILEIRO DE MEDICINA VETERINÁRIA, 31., 2004, São luis. A medicina veterinária no novo milênio: transformação social, preservação ambiental e segurança alimentar: resumos. São Luis, Sociedade Brasileira de Medicina Veterinária, 2004. Seção biotecnologia da reprodução. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 9. | | ORNELLAS, F. L. S.; SOUSA, A. O. de; PIROVANI, C. P.; ARAÚJO, M. do N; COSTA, D. S. da; DANTAS, B. F.; BARBOSA, R. M. Gene expression, biochemical and physiological activities in evaluating melon seed vigor through ethanol release. Scientia Horticulturae, v. 261, 108884, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Semiárido. |
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| 10. | | GONÇALVES, L. P.; ALVES, T. F. O.; MARTINS, C. P. S.; SOUSA, A. O.; GIRARDI, E. A.; GESTEIRA, A. da S.; SOARES FILHO, W. dos S.; COSTA, M. G. C. Analysis of oxidative damage in varieties of Citrus rootstocks subjected to water deficit. WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilhéus. The challenge for the 21st century: book of abstracts. [S.l.]: International Advanced Biology Consortium, 2013.Oline. S02001.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|  |
| 11. | | DÓRIA, M. S.; SOUSA, A. O. de; BARBOSA, C. de J.; COSTA, M. G. C.; GESTEIRA, A. da S.; SOUZA, R. M.; FREITAS, A. C. O.; PIROVANI, C. P. Citrus tristeza virus (CTV) causing proteomic and enzymatic changes in sweet orange variety "Westin". Plos One, July, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 12. | | MENEZES, S. P.; SILVA, D. M. de A.; LIMA, E. M.; SOUSA, A. O. de; ANDRADE, B. S.; LEMOS, L. S. L.; GRAMACHO, A. P.; GESTEIRA, A. da S.; PIROVANI, C. P.; MICHELI, F. The pathogenesis-related protein PR-4b from Theobroma cacao presents RNase activity, Ca2+ and Mg2+ dependent-DNase activity and antifungal action on Moniliophthora pernicios. BMC Plant Biology, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 13. | | GONÇALVES, L. P.; ALVES, T. F. O.; MARTINS, C. P. S.; SOUSA, A. O. de; SANTOS, I. C. dos; PIROVANI, C. P.; ALMEIDA, A-A. F.; COELHO FILHO, M. A.; GESTEIRA, A. da S.; SOARES FILHO, W. dos S.; GIRARDI, E. A.; COSTA, M. G. C. Rootstock-induced physiological and biochemical mechanisms of drought tolerance in sweet orange. Acta Physiologiae Plantarum, June, .38- 174, 2016. 0137-5881| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 13 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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