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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
25/03/2013 |
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Data da última atualização: |
26/03/2013 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
GIROTTO, L.; SOLDERA, M. C. A.; MARIN, S. R. R.; KANAMORI, N.; FUJITA, Y.; YOSHIDA, T.; MARCELINO-GUIMARÃES, F. C.; YAMAGUCHI-SHINOZAKI; NEPOMUCENO, A. L. |
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Afiliação: |
SILVANA REGINA ROCKENBACH MARIN, CNPSO; FRANCISMAR CORREA MARCELINO, CNPSO; ALEXANDRE LIMA NEPOMUCENO, SRI. |
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Título: |
Agrobacterium-mediated transformation of soybean variated, BR16, gene 35S:AREB1, analysis by southern blotting. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: INTERNATIONAL CROP SCIENCE CONGRESS, 6., 2012, Bento Gonçalves. [Proceedings...]. [S.l.]: International Crop Science Society, 2012. Poster, 117. 1 CD-ROM. |
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Idioma: |
Inglês |
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Conteúdo: |
Soybean is the most important source of vegetable oil and protein for human and animal diet in the world. The frequency of occurrence of drought has increased significantly over the last decade, probably associated with climate change occurred due to global warming. Researchers are looking
for a long time to improve and optimize the characteristics of the culture. Several strategies can be used to reduce losses caused by drought, ranging from adequate soil management and agriculture, to the use of irrigation. Another possibility is the development of cultivars adapted to drought
conditions and in this respect, Biotechnology has become an important tool in the development of varieties adapted to different conditions of biotic and abiotic stress. The genetically modified plants can be transformed by direct gene transfer using particle bombardment (biolistics), which inserts a
high number of copies of the transgene into the host genome, resulting many times in deactivation of the transgene by gene silencing. Moreover, the transformed plants via Agrobacterium tumefaciens generally have a low number of copies of the transgene. The use of this method allowed a stable expression and insertion of the interest gene in transgenic plants. The AREB is an important transcription factor signaled by the ABA, which acts in response to regulate the expression of other genes that are involved in tolerance to drought and high salinity stress. This study aimed to analyze the T1 plants transformed Agrobacterium by Southern blotting technique to detect the number of copies of the gene 35S:AREB1. The experiment was conducted at the Laboratory of Plant Biotechnology at Embrapa Soja, Londrina - PR. The process was performed according Kanamori et al., 2011. The Southern blotting was performed on 22 plants T1, AREB positive from two independent events. Of the 22 samples, 20 T1 plants had only one copy number and two plants had two copy numbers. Considering the complexity of the mechanism response to water deficit caused by drought, which can vary depending on the intensity and duration of stress, analysis on the molecular level, physiological and agronomic are needed later to assess the efficiency of the presence of the gene in transformed plants. MenosSoybean is the most important source of vegetable oil and protein for human and animal diet in the world. The frequency of occurrence of drought has increased significantly over the last decade, probably associated with climate change occurred due to global warming. Researchers are looking
for a long time to improve and optimize the characteristics of the culture. Several strategies can be used to reduce losses caused by drought, ranging from adequate soil management and agriculture, to the use of irrigation. Another possibility is the development of cultivars adapted to drought
conditions and in this respect, Biotechnology has become an important tool in the development of varieties adapted to different conditions of biotic and abiotic stress. The genetically modified plants can be transformed by direct gene transfer using particle bombardment (biolistics), which inserts a
high number of copies of the transgene into the host genome, resulting many times in deactivation of the transgene by gene silencing. Moreover, the transformed plants via Agrobacterium tumefaciens generally have a low number of copies of the transgene. The use of this method allowed a stable expression and insertion of the interest gene in transgenic plants. The AREB is an important transcription factor signaled by the ABA, which acts in response to regulate the expression of other genes that are involved in tolerance to drought and high salinity stress. This study aimed to analyze the T1 plants transform... Mostrar Tudo |
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Thesagro: |
Biotecnologia; Soja. |
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Thesaurus Nal: |
Biotechnology; Soybeans. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03123naa a2200265 a 4500
001 1954079
005 2013-03-26
008 2012 bl uuuu u00u1 u #d
100 1 $aGIROTTO, L.
245 $aAgrobacterium-mediated transformation of soybean variated, BR16, gene 35S$bAREB1, analysis by southern blotting.
260 $c2012
520 $aSoybean is the most important source of vegetable oil and protein for human and animal diet in the world. The frequency of occurrence of drought has increased significantly over the last decade, probably associated with climate change occurred due to global warming. Researchers are looking for a long time to improve and optimize the characteristics of the culture. Several strategies can be used to reduce losses caused by drought, ranging from adequate soil management and agriculture, to the use of irrigation. Another possibility is the development of cultivars adapted to drought conditions and in this respect, Biotechnology has become an important tool in the development of varieties adapted to different conditions of biotic and abiotic stress. The genetically modified plants can be transformed by direct gene transfer using particle bombardment (biolistics), which inserts a high number of copies of the transgene into the host genome, resulting many times in deactivation of the transgene by gene silencing. Moreover, the transformed plants via Agrobacterium tumefaciens generally have a low number of copies of the transgene. The use of this method allowed a stable expression and insertion of the interest gene in transgenic plants. The AREB is an important transcription factor signaled by the ABA, which acts in response to regulate the expression of other genes that are involved in tolerance to drought and high salinity stress. This study aimed to analyze the T1 plants transformed Agrobacterium by Southern blotting technique to detect the number of copies of the gene 35S:AREB1. The experiment was conducted at the Laboratory of Plant Biotechnology at Embrapa Soja, Londrina - PR. The process was performed according Kanamori et al., 2011. The Southern blotting was performed on 22 plants T1, AREB positive from two independent events. Of the 22 samples, 20 T1 plants had only one copy number and two plants had two copy numbers. Considering the complexity of the mechanism response to water deficit caused by drought, which can vary depending on the intensity and duration of stress, analysis on the molecular level, physiological and agronomic are needed later to assess the efficiency of the presence of the gene in transformed plants.
650 $aBiotechnology
650 $aSoybeans
650 $aBiotecnologia
650 $aSoja
700 1 $aSOLDERA, M. C. A.
700 1 $aMARIN, S. R. R.
700 1 $aKANAMORI, N.
700 1 $aFUJITA, Y.
700 1 $aYOSHIDA, T.
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aYAMAGUCHI-SHINOZAKI
700 1 $aNEPOMUCENO, A. L.
773 $tIn: INTERNATIONAL CROP SCIENCE CONGRESS, 6., 2012, Bento Gonçalves. [Proceedings...]. [S.l.]: International Crop Science Society, 2012. Poster, 117. 1 CD-ROM.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 14 | |
| 1. |  | KAJIWARA, T. H.; SOLDERA, M. C. A.; URQUIZA, G. P. C.; VIEIRA, N. D.; ALMEIDA, A. M. R. Diversidade genética de isolados de Corynespora cassiicola (Berk. & Curt.) Wei, por RAPD e PCR-RFLP. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 4., 2009, Londrina. Resumos... Londrina: Embrapa Soja, 2009. p. 103-106. (Embrapa Soja. Documentos, 312). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 2. | | GIROTTO, L.; SOLDERA, M. C. A.; MARIN, S. R. R.; KANAMORI, N.; FUJITA, Y.; YOSHIDA, T.; MARCELINO-GUIMARÃES, F. C.; YAMAGUCHI-SHINOZAKI; NEPOMUCENO, A. L. Agrobacterium-mediated transformation of soybean variated, BR16, gene 35S:AREB1, analysis by southern blotting. In: INTERNATIONAL CROP SCIENCE CONGRESS, 6., 2012, Bento Gonçalves. [Proceedings...]. [S.l.]: International Crop Science Society, 2012. Poster, 117. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 3. | | SOLDERA, M. C. A.; LAURINDO, D. G.; BALESTRI, M. R. D.; MORAES, A. M.; MARIN, S. R. R.; BINNEK, E.; COSTAMILAN, L. M. Diversidade genética do complexo Diaporthe/Phomopsis em soja, no Brasil. Fitopatologia Brasileira, Brasília, DF, v. 32, p. S197, ago. 2007. Suplemento, ref. 0436. Edição dos Resumos do XL Congresso Brasileiro de Fitopatologia, Maringá, ago. 2007.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Trigo. |
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| 4. | | SOLDERA, M. C. A. VIEIRA, N. D.; LAURINDO, D. G.; MORALES, A. M.; MARIN, S. R. R.; BINNEK, E.; ALMEIDA, A. M. R. Diversidade genética do complexo diaporthe/phomopsis em soja, no Brasil. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 2008, Londrina. Resumos expandidos. ..Londrina: Embrapa Soja, 2008. p. 190-194 (Embrapa Soja. Documentos, 297).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 5. | | BALESTRI, M. R. D.; LAURINDO, D. G.; SOLDERA, M. C. A.; SIMIONATO, A. A.; COSTA, J. M.; ALMEIDA, A. M. R. Efeito da rotação de culturas e semeadura direta sobre patógenos radiculares da soja. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 3., 2008, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2008. p. 17-21 (Embrapa Soja. Documentos, 297).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 6. | | BALESTRI, M. R. D.; LAURINDO, D. G.; SOLDERA, M. C. A.; SIMIONATO, A. A. COSTA, J. M.; COSTA, J. M.; ALMEIDA, A. M. R. Efeito da rotação de culturas e semeadura direta sobre patógenos radiculares da soja. Fitopatologia Brasileira, Brasília, DF, v. 32, p. S302, ago. 2007. Suplemento, resumo 0970. Edição dos Resumos do XL Congresso Brasileiro de Fitopatologia, Maringá, PR, ago. 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Soja. |
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| 7. | | HONNA, P. T.; GIROTTO, L.; SOLDERA, M. C. A.; KANAMORI, N.; MARCELINO-GUIMARÃES, F. C.; YAMAGUCHI-SHINOZAKI, K.; FARIAS, J. R. B. Identificação de sementes de soja geneticamente modificadas utilizando a técnica de PCR convencional. JORNADA ACADÊMICA DA EMBRAPA SOJA, 7., 2012, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2012. p. 134-138. (Embrapa Soja. Documentos, 333). Editado por Paula Gerón Saiz de Mello. Disponível em: .| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 8. | | LAURINDO, D. G.; SOLDERA, M. C. A.; BALESTRI, M. R. D.; HOFFMANN, L. L.; MEYER, M. C.; ALMEIDA, A. M. R. Identificação do Cowpea mild mottle virus em soja no município de Sorriso, Mato Grosso. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 3., 2008, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2008. p. 26-30 (Embrapa Soja. Documentos, 297).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 9. | | LAURINDO, D. G.; SOLDERA, M. C. A.; BALESTRI, M. R. D.; HOFFMANN, L. L.; MEYER, M. C.; ALMEIDA, A. M. R. Identificação do Cowpea mild mottle virus em soja no município de Sorriso, MT. Fitopatologia Brasileira, Brasília, DF, v. 32, p. S227, ago. 2007. Suplemento, resumo 0589. Edição dos Resumos do XL Congresso Brasileiro de Fitopatologia, Maringá, PR, ago. 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Soja. |
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| 10. | | SANTOS, P. J. C.; BENATO, L. C.; SOUZA, N. V.; SOLDERA, M. C. A.; VIEIRA, N. D.; ALMEIDA, A. M. R. Relação da profundidade com o sistema de cultivo sobre a concentração de microesclerócios de Macrophomina phaseolina. Tropical Plant Pathology, Brasília, DF, v. 34, p. S134, ago. 2009. Suplemento, ref. 489. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia; Annual Meeting of the Brazilian Phytopathological Society, Rio de Janeiro, ago. 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 11. | | GIROTTO, L.; SOLDERA, M. C. A.; HONNA, P. T.; KANAMORI, N.; MARCELINO-GUIMARÃES, F. C.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L. Transformação da cultivar de soja BR 16 via Agrobacterium tumefaciens, com a construção 35S: AREB1. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: resumos. Brasília, DF: Embrapa, 2012. p. 15, res. 7.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 12. | | GIROTTO, L.; SOLDERA, M. C. A.; HONNA, P. T.; KANAMORI, N.; MARCELINO-GUIMARÃES, F. C.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L. Transformação da cultivar de soja BR 16 via Agrobacterium tumefaciens, com a construção 35S:AREB1. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: anais. Brasília, DF: Embrapa, 2012. 3 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 13. | | SOLDERA, M. C. A.; LAURINDO, D. G.; BALESTRI, M. R. D.; MORAES, A. M.; MARIN, S. R. R.; BINNECK, E.; COSTAMILAN, L. M.; JACCOUD FILHO, D.; ÁLMEIDA, A. M. R. Diversidade genética do complexo Diaphorte/Phomopsis em soja, no Brasil. Fitopatologia Brasileira, Brasília, DF, v. 32, p. S197, ago. 2007. Suplemento, resumo 0436. Edição dos Resumos do XL Congresso Brasileiro de Fitopatologia, Maringá, PR, ago. 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Soja. |
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| 14. | | SANTOS, P. J. C.; BENATO, L. C.; SOUZA, N. V.; SOLDERA, M. C. A.; VIEIRA, N. D.; OLIVEIRA, M. C. N. de; SOUTO, E. R.; ALMEIDA, A. M. R. Relação da profundidade com o sistema de cultivo sobre a concentração de microesclerócios de Macrophomina phaseolina. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 4., 2009, Londrina. Resumos... Londrina: Embrapa Soja, 2009. p. 286-289. (Embrapa Soja. Documentos, 312). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 14 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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