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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
15/12/2021 |
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Data da última atualização: |
23/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NAKAI, D. K.; RIBEIRO, J. A. de A.; MARTINS, P. A.; SOARES, I. P.; SALUM, T. F. C.; COSTA, P. P. K. G. |
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Afiliação: |
DIOGO KEIJI NAKAI, CNPAE; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; PEDRO A. MARTINS, Universidade de Brasília; ITANIA PINHEIRO SOARES, CNPAE; THAIS FABIANA CHAN SALUM, CNPAE; PATRICIA PINTO KALIL G COSTA, CNPAE. |
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Título: |
Evaporative light scattering detection based reversed-phase ultra-high-performance liquid chromatography method to quantify intermediates and end products of biodiesel production. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Journal of Chromatography A, v. 1663, 462726, 2022. |
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ISSN: |
0021-9673 |
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DOI: |
https://doi.org/10.1016/j.chroma.2021.462726 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: A fast method based on reversed-phase ultra-high-performance liquid chromatography using evaporative light scattering detection (RP-UHPLC-ELSD) was developed for monitoring the intermediates and end products of biodiesel production. Gradient elution of water, acetonitrile, and a mixture of acetonitrile:2-propanol:n-hexane was used. With a minimal and easy sample preparation, fatty acid methyl esters (FAME), fatty acid ethyl esters (FAEE), free fatty acids (FFA), monoacylglycerols (MAG), diacylglycerols (DAG), and triacylglycerols (TAG) were successfully separated. The developed method was used to monitor an ethylic enzymatic transesterification of soybean oil and to characterize the ester content of methyl and ethyl biodiesel. The ester content obtained was compared with the reference method by gas chromatography and flame ionization detector (GC-FID), with similar results for both methyl and ethyl biodiesel. The presented method is a simple and fast alternative, a 17-minutes run, to monitor the transesterification process, simultaneously quantifying all the analytes produced in the reaction: biodiesel and its intermediates. Limits of detection (LOD, between 29 and 307 ng) and quantification (LOQ, between 48 and 614 ng), linearity (R2>0.99), precision (between 0.30 and 6.58%), and accuracy (between 81.6 and 119.9%) were determined for the twenty-one compounds. |
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Palavras-Chave: |
RP-UHPLC-ELSD; UHPLC. |
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Thesaurus Nal: |
Acylglycerols; Biodiesel; Esters; Free fatty acids. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02291naa a2200277 a 4500
001 2137855
005 2022-11-23
008 2022 bl uuuu u00u1 u #d
022 $a0021-9673
024 7 $ahttps://doi.org/10.1016/j.chroma.2021.462726$2DOI
100 1 $aNAKAI, D. K.
245 $aEvaporative light scattering detection based reversed-phase ultra-high-performance liquid chromatography method to quantify intermediates and end products of biodiesel production.$h[electronic resource]
260 $c2022
520 $aAbstract: A fast method based on reversed-phase ultra-high-performance liquid chromatography using evaporative light scattering detection (RP-UHPLC-ELSD) was developed for monitoring the intermediates and end products of biodiesel production. Gradient elution of water, acetonitrile, and a mixture of acetonitrile:2-propanol:n-hexane was used. With a minimal and easy sample preparation, fatty acid methyl esters (FAME), fatty acid ethyl esters (FAEE), free fatty acids (FFA), monoacylglycerols (MAG), diacylglycerols (DAG), and triacylglycerols (TAG) were successfully separated. The developed method was used to monitor an ethylic enzymatic transesterification of soybean oil and to characterize the ester content of methyl and ethyl biodiesel. The ester content obtained was compared with the reference method by gas chromatography and flame ionization detector (GC-FID), with similar results for both methyl and ethyl biodiesel. The presented method is a simple and fast alternative, a 17-minutes run, to monitor the transesterification process, simultaneously quantifying all the analytes produced in the reaction: biodiesel and its intermediates. Limits of detection (LOD, between 29 and 307 ng) and quantification (LOQ, between 48 and 614 ng), linearity (R2>0.99), precision (between 0.30 and 6.58%), and accuracy (between 81.6 and 119.9%) were determined for the twenty-one compounds.
650 $aAcylglycerols
650 $aBiodiesel
650 $aEsters
650 $aFree fatty acids
653 $aRP-UHPLC-ELSD
653 $aUHPLC
700 1 $aRIBEIRO, J. A. de A.
700 1 $aMARTINS, P. A.
700 1 $aSOARES, I. P.
700 1 $aSALUM, T. F. C.
700 1 $aCOSTA, P. P. K. G.
773 $tJournal of Chromatography A$gv. 1663, 462726, 2022.
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