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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
08/12/2021 |
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Data da última atualização: |
08/12/2021 |
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Tipo da produção científica: |
Orientação de Tese de Pós-Graduação |
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Autoria: |
SOARES, C. E. V. F. |
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Afiliação: |
CARLOS EMANOEL VIEIRA FLORES SOARES, Universidade de Brasília. |
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Título: |
Caracterização fisiológica e comparação de leveduras Saccharomyces e não-Saccharomyces na presença de diferentes inibidores presentes no hidrolisado lignocelulósico. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Brasília, DF, 2021. |
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Páginas: |
86 p. |
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Descrição Física: |
PDF: il. color. |
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Idioma: |
Português |
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Notas: |
Tese (Doutorado em Tecnologias Químicas e Biológicas) - Universidade de Brasília, Instituto de Química, Programa de Pós-Graduação em Tecnologias Químicas e Biológicas, Brasília, 2020. Orientador: Professor doutor João Ricardo Moreira de Almeida (pesquisador da Embrapa Agroenergia). |
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Conteúdo: |
Resumo: Várias espécies de leveduras tem sido avaliadas para a produção de combustíveis e produtos químicos a partir de açúcares presentes na biomassa lignocelulósica. O hidrolisado lignocelulósico contém açúcares e também compostos que inibem o metabolismo microbiano, como ácidos orgânicos, furaldeídos e compostos fenólicos. Compreender a resposta de leveduras a esses compostos inibitórios é importante para o desenvolvimento de bioprocessos. Nesse contexto, o objetivo geral desse trabalho foi identificar novas linhagens de leveduras capazes de metabolizar xilose e caracterizar a resposta fisiológica de diferentes espécies em termos de tolerância a inibidores presentes em hidrolisados lignocelulósicos. Inicialmente, as linhagens de leveduras JA1 e JA9, previamente isoladas de madeira em decomposição, foram identificadas pela análise de dados de sequência de DNA das regiões D1/D2 e região ITS 5.8S. Em seguida, o potencial de assimilação de xilose e produção de xilitol dessas linhagens foi avaliado em meio contendo xilose, mistura de xilose-glicose e em hidrolisado lignocelulósico. As linhagens foram identificadas como Spathaspora sp. JA1 e Meyerozyma caribbica JA9, e ambas se destacaram pela capacidade de produção de xilitol em meio sintético e em hidrolisado de biomassa de cana de açúcar. De fato, Spathaspora sp. JA1 produziu 22,62 g/L de xilitol, com rendimento de 0,58 g/g em hidrolisado. Em um segundo momento, a tolerância e a resposta fisiológica de 7 leveduras industriais de Saccharomyces cerevisiae e 7 leveduras não-Saccharomyces em relação a inibidores selecionados de hidrolisados lignocelulósicos foram avaliadas. Comparou-se o perfil de crescimento das 14 leveduras na presença de três concentrações diferentes de furaldeídos (furfural e 5-hidroximetil- furfural - HMF), ácidos orgânicos (ácido acético e ácido fórmico) e compostos fenólicos (vanilina, seringaldeido, ácidos ferúlico e ácido cumárico). Baseado no perfil de crescimento das leveduras, 7 delas foram selecionadas para análise do perfil fermentativo na presença de ácido acético, HMF e vanilina, os compostos inibidores mais tóxicos nas condições testadas. Candida tropicalis JA2, Meyerozyma caribbica JA9, Wickerhamomyces anomalus 740, S. cerevisiae JP1, B1.1 e G06 foram selecionadas porque se mostraram mais tolerantes aos compostos testados, enquanto Spathaspora sp. JA1 foi selecionada por seu bom desempenho no consumo de xilose e produção de xilitol. Os resultados obtidos mostraram uma resposta dose-dependente das leveduras em relação aos oito inibidores avaliados. Entre as leveduras comparadas, as linhagens de S. cerevisiae apresentaram maior tolerância aos compostos fenólicos e furaldeídos, 3 delas com maior tolerância que as demais. Com relação às leveduras não-Saccharomyces, C. tropicalis JA2 e W. anomalus 740 apareceram como as mais tolerantes, enquanto as cepas de Spathaspora foram as mais sensíveis aos diferentes inibidores. Esses resultados demonstram a importância da caracterização fisiológica de diferentes linhagens e espécies de leveduras Saccharomyces e não-Saccharomyces. Abstract: Several yeast species were evaluated for the production of fuels and chemicals from sugars present in lignocellulosic biomass. The lignocellulosic hydrolysate also contains compounds that inhibit microbial metabolism, such as organic acids, furaldehydes and phenolic compounds. Understanding the response of yeasts to these inhibitory compounds is important for the development of different bioprocesses. The general objective of this work was to identify new yeast strains capable of metabolizing xylose and to characterize the physiological response of different species in terms of tolerance to inhibitors present in lignocellulosic hydrolysates. Initially, the yeast strains JA1 and JA9, previously isolated from decomposing wood, were identified by analyzing DNA sequence data from D1/D2 regions and ITS 5.8S region. Then, the potential for assimilation of xylose and production of xylitol from these strains was evaluated in a medium containing xylose, a mixture of xylose-glucose and in lignocellulosic hydrolysate. The strains were identified as Spathaspora sp. JA1 and Meyerozyma caribbica JA9, and both stood out for their capacity to produce xylitol in synthetic medium and in sugarcane biomass hydrolysate. In fact, Spathaspora sp. JA1 produced 22.62 g/L of xylitol, with yield of 0.58 g/g in hydrolysate. In a second step, the tolerance and physiological response of 7 industrial yeasts of Saccharomyces cerevisiae and 7 non-Saccharomyces yeasts in relation to selected inhibitors of lignocellulosic hydrolysates were evaluated. The growth profile of the 14 yeasts was compared in the presence of three different concentrations of furaldehydes (furfural and 5-hydroxymethyl-furfural - HMF), organic acids (acetic acid and formic acid) and phenolic compounds (vanillin, syringaldehyde, ferulic acids and coumaric acid). Based on the yeast growth profile, 7 yeasts were selected for analysis of the fermentative profile in the presence of acetic acid, HMF and vanillin, the most toxic inhibitory compounds under the conditions tested. Candida tropicalis JA2, M. caribbica JA9,Wickerhamomyces anomalus 740, S. cerevisiae JP1, B1.1 and G06 were selected because they were more tolerant to the compounds tested, while Spathaspora sp. JA1 was selected for its good performance in the consumption of xylose. The results obtained showed a dose-dependent response of yeasts in relation to the eight inhibitors evaluated. Among the yeasts compared, the strains of S. cerevisiae showed greater tolerance to phenolic and furaldehydes compounds, 3 of them with greater tolerance than the others. Regarding non-Saccharomyces yeasts, C.tropicalis JA2 and W. anomalus 740 appeared as the most tolerant, while the strains of Spathaspora were the most sensitive to different inhibitors. These results demonstrate the importance of the physiological characterization of different Saccharomyces and non- Saccharomyces yeasts and species. MenosResumo: Várias espécies de leveduras tem sido avaliadas para a produção de combustíveis e produtos químicos a partir de açúcares presentes na biomassa lignocelulósica. O hidrolisado lignocelulósico contém açúcares e também compostos que inibem o metabolismo microbiano, como ácidos orgânicos, furaldeídos e compostos fenólicos. Compreender a resposta de leveduras a esses compostos inibitórios é importante para o desenvolvimento de bioprocessos. Nesse contexto, o objetivo geral desse trabalho foi identificar novas linhagens de leveduras capazes de metabolizar xilose e caracterizar a resposta fisiológica de diferentes espécies em termos de tolerância a inibidores presentes em hidrolisados lignocelulósicos. Inicialmente, as linhagens de leveduras JA1 e JA9, previamente isoladas de madeira em decomposição, foram identificadas pela análise de dados de sequência de DNA das regiões D1/D2 e região ITS 5.8S. Em seguida, o potencial de assimilação de xilose e produção de xilitol dessas linhagens foi avaliado em meio contendo xilose, mistura de xilose-glicose e em hidrolisado lignocelulósico. As linhagens foram identificadas como Spathaspora sp. JA1 e Meyerozyma caribbica JA9, e ambas se destacaram pela capacidade de produção de xilitol em meio sintético e em hidrolisado de biomassa de cana de açúcar. De fato, Spathaspora sp. JA1 produziu 22,62 g/L de xilitol, com rendimento de 0,58 g/g em hidrolisado. Em um segundo momento, a tolerância e a resposta fisiológica de 7 leveduras industriai... Mostrar Tudo |
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Palavras-Chave: |
Hidrolisado; Inibidores; Leveduras; Não-Saccharomyces; Tolerância; Xilose. |
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Thesaurus Nal: |
Enzyme inhibitors; Hydrolysates; Lignocellulose; Saccharomyces; Strains; Xylose. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/228742/1/Tese-Caracterizac807a771o-fisiolo769gica-e-comparac807a771o.pdf
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Marc: |
LEADER 07242nam a2200277 a 4500
001 2137344
005 2021-12-08
008 2021 bl uuuu m 00u1 u #d
100 1 $aSOARES, C. E. V. F.
245 $aCaracterização fisiológica e comparação de leveduras Saccharomyces e não-Saccharomyces na presença de diferentes inibidores presentes no hidrolisado lignocelulósico.$h[electronic resource]
260 $aBrasília, DF$c2021
300 $a86 p.$cPDF: il. color.
500 $aTese (Doutorado em Tecnologias Químicas e Biológicas) - Universidade de Brasília, Instituto de Química, Programa de Pós-Graduação em Tecnologias Químicas e Biológicas, Brasília, 2020. Orientador: Professor doutor João Ricardo Moreira de Almeida (pesquisador da Embrapa Agroenergia).
520 $aResumo: Várias espécies de leveduras tem sido avaliadas para a produção de combustíveis e produtos químicos a partir de açúcares presentes na biomassa lignocelulósica. O hidrolisado lignocelulósico contém açúcares e também compostos que inibem o metabolismo microbiano, como ácidos orgânicos, furaldeídos e compostos fenólicos. Compreender a resposta de leveduras a esses compostos inibitórios é importante para o desenvolvimento de bioprocessos. Nesse contexto, o objetivo geral desse trabalho foi identificar novas linhagens de leveduras capazes de metabolizar xilose e caracterizar a resposta fisiológica de diferentes espécies em termos de tolerância a inibidores presentes em hidrolisados lignocelulósicos. Inicialmente, as linhagens de leveduras JA1 e JA9, previamente isoladas de madeira em decomposição, foram identificadas pela análise de dados de sequência de DNA das regiões D1/D2 e região ITS 5.8S. Em seguida, o potencial de assimilação de xilose e produção de xilitol dessas linhagens foi avaliado em meio contendo xilose, mistura de xilose-glicose e em hidrolisado lignocelulósico. As linhagens foram identificadas como Spathaspora sp. JA1 e Meyerozyma caribbica JA9, e ambas se destacaram pela capacidade de produção de xilitol em meio sintético e em hidrolisado de biomassa de cana de açúcar. De fato, Spathaspora sp. JA1 produziu 22,62 g/L de xilitol, com rendimento de 0,58 g/g em hidrolisado. Em um segundo momento, a tolerância e a resposta fisiológica de 7 leveduras industriais de Saccharomyces cerevisiae e 7 leveduras não-Saccharomyces em relação a inibidores selecionados de hidrolisados lignocelulósicos foram avaliadas. Comparou-se o perfil de crescimento das 14 leveduras na presença de três concentrações diferentes de furaldeídos (furfural e 5-hidroximetil- furfural - HMF), ácidos orgânicos (ácido acético e ácido fórmico) e compostos fenólicos (vanilina, seringaldeido, ácidos ferúlico e ácido cumárico). Baseado no perfil de crescimento das leveduras, 7 delas foram selecionadas para análise do perfil fermentativo na presença de ácido acético, HMF e vanilina, os compostos inibidores mais tóxicos nas condições testadas. Candida tropicalis JA2, Meyerozyma caribbica JA9, Wickerhamomyces anomalus 740, S. cerevisiae JP1, B1.1 e G06 foram selecionadas porque se mostraram mais tolerantes aos compostos testados, enquanto Spathaspora sp. JA1 foi selecionada por seu bom desempenho no consumo de xilose e produção de xilitol. Os resultados obtidos mostraram uma resposta dose-dependente das leveduras em relação aos oito inibidores avaliados. Entre as leveduras comparadas, as linhagens de S. cerevisiae apresentaram maior tolerância aos compostos fenólicos e furaldeídos, 3 delas com maior tolerância que as demais. Com relação às leveduras não-Saccharomyces, C. tropicalis JA2 e W. anomalus 740 apareceram como as mais tolerantes, enquanto as cepas de Spathaspora foram as mais sensíveis aos diferentes inibidores. Esses resultados demonstram a importância da caracterização fisiológica de diferentes linhagens e espécies de leveduras Saccharomyces e não-Saccharomyces. Abstract: Several yeast species were evaluated for the production of fuels and chemicals from sugars present in lignocellulosic biomass. The lignocellulosic hydrolysate also contains compounds that inhibit microbial metabolism, such as organic acids, furaldehydes and phenolic compounds. Understanding the response of yeasts to these inhibitory compounds is important for the development of different bioprocesses. The general objective of this work was to identify new yeast strains capable of metabolizing xylose and to characterize the physiological response of different species in terms of tolerance to inhibitors present in lignocellulosic hydrolysates. Initially, the yeast strains JA1 and JA9, previously isolated from decomposing wood, were identified by analyzing DNA sequence data from D1/D2 regions and ITS 5.8S region. Then, the potential for assimilation of xylose and production of xylitol from these strains was evaluated in a medium containing xylose, a mixture of xylose-glucose and in lignocellulosic hydrolysate. The strains were identified as Spathaspora sp. JA1 and Meyerozyma caribbica JA9, and both stood out for their capacity to produce xylitol in synthetic medium and in sugarcane biomass hydrolysate. In fact, Spathaspora sp. JA1 produced 22.62 g/L of xylitol, with yield of 0.58 g/g in hydrolysate. In a second step, the tolerance and physiological response of 7 industrial yeasts of Saccharomyces cerevisiae and 7 non-Saccharomyces yeasts in relation to selected inhibitors of lignocellulosic hydrolysates were evaluated. The growth profile of the 14 yeasts was compared in the presence of three different concentrations of furaldehydes (furfural and 5-hydroxymethyl-furfural - HMF), organic acids (acetic acid and formic acid) and phenolic compounds (vanillin, syringaldehyde, ferulic acids and coumaric acid). Based on the yeast growth profile, 7 yeasts were selected for analysis of the fermentative profile in the presence of acetic acid, HMF and vanillin, the most toxic inhibitory compounds under the conditions tested. Candida tropicalis JA2, M. caribbica JA9,Wickerhamomyces anomalus 740, S. cerevisiae JP1, B1.1 and G06 were selected because they were more tolerant to the compounds tested, while Spathaspora sp. JA1 was selected for its good performance in the consumption of xylose. The results obtained showed a dose-dependent response of yeasts in relation to the eight inhibitors evaluated. Among the yeasts compared, the strains of S. cerevisiae showed greater tolerance to phenolic and furaldehydes compounds, 3 of them with greater tolerance than the others. Regarding non-Saccharomyces yeasts, C.tropicalis JA2 and W. anomalus 740 appeared as the most tolerant, while the strains of Spathaspora were the most sensitive to different inhibitors. These results demonstrate the importance of the physiological characterization of different Saccharomyces and non- Saccharomyces yeasts and species.
650 $aEnzyme inhibitors
650 $aHydrolysates
650 $aLignocellulose
650 $aSaccharomyces
650 $aStrains
650 $aXylose
653 $aHidrolisado
653 $aInibidores
653 $aLeveduras
653 $aNão-Saccharomyces
653 $aTolerância
653 $aXilose
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Embrapa Agroenergia (CNPAE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
18/05/2023 |
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Data da última atualização: |
12/04/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CORREA-FILHO, L.; SANTOS JUNIOR, J. R.; BARBOZA, H. T. G.; SOARES, A. G.; TONON, R. V.; CABRAL, L. M. C. |
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Afiliação: |
LUIZ CORREA-FILHO; JAILTON RIBEIRO DOS SANTOS JUNIOR, UFRRJ; HENRIQUETA TALITA GUIMARAES BARBOZA, CTAA; ANTONIO GOMES SOARES, CTAA; RENATA VALERIANO TONON, CTAA; LOURDES MARIA CORREA CABRAL, CTAA. |
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Título: |
Potential of chitosan-alginate edible films as packaging for fish meat. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: CONFERÊNCIA INTERNACIONAL DE PROTEÍNAS E COLOIDES ALIMENTARES, 9., 2023, Rio de Janeiro. Anais... Campinas, Galoá, 2023. |
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Idioma: |
Inglês |
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Notas: |
Poster 157740. CIPCA. |
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Conteúdo: |
Consumers, increasingly aware and informed, tend to demand safe products with proven quality, obtained from good manufacturing and risk control practices. In this context, research has been carried out aimed at replacing synthetic additives with more natural elements, less harmful to consumer health and the environment, whether chemical or microbiological changes. Some animal products like fishs are highly perishable foods, susceptible to rapid microbiological deterioration during storage. In this way, this work aims to develop active chitosan/alginate films incorporated with zinc oxide (ZnO) nanoparticles, aiming to provide an alternative for preserving and increasing the fish shelf life. Films were produced by casting. The polymer concentration in the filmogenic solutions was 1.5%w/v in the following proportions of alginate:chitosan: 100:0; 25:75; 50:50; 75:25 and 0:100. Chitosan solutions were prepared in 1%v/v aqueous acetic acid under magnetic stirring. The alginate was dissolved in distilled water at 60°C under mechanical agitation at 6,500 rpm. Thereafter, a crosslinking with calcium chloride at 1.0% w/w was performed. In all polymeric solutions, 30%w/w glycerol was added. The polymer solutions were poured in an acrylic plate and dried at 35°C for 48 h. The films were characterized regarding chemical, mechanical and barrier properties. All the evaluated formulations resulted in non-rigid and homogeneous films. Alginate and chitosan composite films were less transparent than the single films. Chitosan films were less water soluble than the alginate ones. Films with 100% alginate were completely water-soluble. The sorption degree was in line with the solubility results, i.e., the higher the proportion of chitosan, the lower the sorption degree. Elongation and tensile strength values ranged from 3.67 to 17.87% and 16.10 to 37.87 MPa, respectively. It was observed that the increase in alginate concentration led to an increase in the tensile strength and a reduction in elongation at break. Overall, films produced with more than 75% chitosan concentration showed better potential barrier and mechanical properties, mainly low water solubility and higher elongation at break, being suitable for use in formulations intended to produce edible films. It is expected that films with a higher concentration of chitosan have good potential to conduct the study of films incorporated with ZnO nanoparticles. MenosConsumers, increasingly aware and informed, tend to demand safe products with proven quality, obtained from good manufacturing and risk control practices. In this context, research has been carried out aimed at replacing synthetic additives with more natural elements, less harmful to consumer health and the environment, whether chemical or microbiological changes. Some animal products like fishs are highly perishable foods, susceptible to rapid microbiological deterioration during storage. In this way, this work aims to develop active chitosan/alginate films incorporated with zinc oxide (ZnO) nanoparticles, aiming to provide an alternative for preserving and increasing the fish shelf life. Films were produced by casting. The polymer concentration in the filmogenic solutions was 1.5%w/v in the following proportions of alginate:chitosan: 100:0; 25:75; 50:50; 75:25 and 0:100. Chitosan solutions were prepared in 1%v/v aqueous acetic acid under magnetic stirring. The alginate was dissolved in distilled water at 60°C under mechanical agitation at 6,500 rpm. Thereafter, a crosslinking with calcium chloride at 1.0% w/w was performed. In all polymeric solutions, 30%w/w glycerol was added. The polymer solutions were poured in an acrylic plate and dried at 35°C for 48 h. The films were characterized regarding chemical, mechanical and barrier properties. All the evaluated formulations resulted in non-rigid and homogeneous films. Alginate and chitosan composite films were less transparen... Mostrar Tudo |
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Palavras-Chave: |
Antimicrobial activity; Biodegradable films. |
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Thesaurus NAL: |
Zinc oxide. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03168nam a2200217 a 4500
001 2153809
005 2024-04-12
008 2023 bl uuuu u00u1 u #d
100 1 $aCORREA-FILHO, L.
245 $aPotential of chitosan-alginate edible films as packaging for fish meat.$h[electronic resource]
260 $aIn: CONFERÊNCIA INTERNACIONAL DE PROTEÍNAS E COLOIDES ALIMENTARES, 9., 2023, Rio de Janeiro. Anais... Campinas, Galoá$c2023
500 $aPoster 157740. CIPCA.
520 $aConsumers, increasingly aware and informed, tend to demand safe products with proven quality, obtained from good manufacturing and risk control practices. In this context, research has been carried out aimed at replacing synthetic additives with more natural elements, less harmful to consumer health and the environment, whether chemical or microbiological changes. Some animal products like fishs are highly perishable foods, susceptible to rapid microbiological deterioration during storage. In this way, this work aims to develop active chitosan/alginate films incorporated with zinc oxide (ZnO) nanoparticles, aiming to provide an alternative for preserving and increasing the fish shelf life. Films were produced by casting. The polymer concentration in the filmogenic solutions was 1.5%w/v in the following proportions of alginate:chitosan: 100:0; 25:75; 50:50; 75:25 and 0:100. Chitosan solutions were prepared in 1%v/v aqueous acetic acid under magnetic stirring. The alginate was dissolved in distilled water at 60°C under mechanical agitation at 6,500 rpm. Thereafter, a crosslinking with calcium chloride at 1.0% w/w was performed. In all polymeric solutions, 30%w/w glycerol was added. The polymer solutions were poured in an acrylic plate and dried at 35°C for 48 h. The films were characterized regarding chemical, mechanical and barrier properties. All the evaluated formulations resulted in non-rigid and homogeneous films. Alginate and chitosan composite films were less transparent than the single films. Chitosan films were less water soluble than the alginate ones. Films with 100% alginate were completely water-soluble. The sorption degree was in line with the solubility results, i.e., the higher the proportion of chitosan, the lower the sorption degree. Elongation and tensile strength values ranged from 3.67 to 17.87% and 16.10 to 37.87 MPa, respectively. It was observed that the increase in alginate concentration led to an increase in the tensile strength and a reduction in elongation at break. Overall, films produced with more than 75% chitosan concentration showed better potential barrier and mechanical properties, mainly low water solubility and higher elongation at break, being suitable for use in formulations intended to produce edible films. It is expected that films with a higher concentration of chitosan have good potential to conduct the study of films incorporated with ZnO nanoparticles.
650 $aZinc oxide
653 $aAntimicrobial activity
653 $aBiodegradable films
700 1 $aSANTOS JUNIOR, J. R.
700 1 $aBARBOZA, H. T. G.
700 1 $aSOARES, A. G.
700 1 $aTONON, R. V.
700 1 $aCABRAL, L. M. C.
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