Data/hora: 22/09/2024 19:49:51

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	09/11/1999	
Data da última atualização: 	09/11/1999	
Autoria: 	FITCH, M. M. M.; MANSHARDT, R. M.; GONSALVES, D.; SLIGHTOM, J. L.; SANFORD, J. C.	
Afiliação: 	Department of Horticulture, University of Hawaii, Honolulu; Department of Agriculture, ARS; Department of Plant Pathology Cornell, University, Geneva, New York; Department of Horticultural Sciences.	
Título: 	Virus resistant papaya plants derived from tissues bombarded with the coat protein gene of papaya ringspot virus	
Ano de publicação: 	1992	
Fonte/Imprenta: 	Bio Tecnology, v.10, n.11, p.14466-1472, 1992	
Idioma: 	Inglês	
Conteúdo: 	Papaya ringspot virus (PRV) is a serious disease of papaya (Carica papaya L.) that has only been partially controlled by conventional methods. An alternative control method is coat protein-mediated protection (CPMP) through the trans fer and expression of the PRV coat protein (Cp) gene in papaya. We report an efficient gene transfer system utilizing microprojectile-mediated transformation of 2,4-D-treated immature zygotic embryos with a plasmid construction that contains the neomycin phosphotransferase II (NPTII) and B-glucuronidase (GUS) genes flanking a PRV cp gene expression cassette. Putative transgenic R papaya plants, regenerated on kanamycin-containing medium, were assayed for GUS and PRV coat protein expression, for the presence of NPTII and PRV cp genes [with the polymerase chain reaction (PCR) and genomic blot hybridization analysis], and for PRV cp gene transcripts by Northern analysis.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (17412-1) - ADD - SP - -- - S11580 -  - S11580 - DIS