|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
07/12/2011 |
Data da última atualização: |
05/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LESSARD, C.; SIQUEIRA, L. G. B.; D'AMOURS, O.; SULLIVAN, R.; LECLERC, P.; PALMER, C. |
Afiliação: |
C. LESSARD, Agriculture and Agri-Food Canada, Western College of Veterinary Medicine; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; O. D'AMOURS, Université Laval, Canada; R. SULLIVAN, Université Laval, Canada; P. LECLERC, Université Laval, Canada; C. PALMER, University of Saskatchewan, Canada. |
Título: |
Infertility in a beef bull due to a failure in the capacitation process. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Theriogenology, v. 76, n. 5, p. 891-899, 2011. |
DOI: |
https://doi.org/10.1016/j.theriogenology.2011.04.020 |
Idioma: |
Inglês |
Conteúdo: |
The objective of this case report was to identify the cause of apparent idiopathic infertility in a Red Angus (beef) bull. Semen was collected by electroejaculation and submitted to a series of assays, including evaluation of sperm motility by computer-assisted sperm analysis (CASA), sperm morphology and DNA integrity, semen cryopreservation, AI, IVF, induction of the acrosome reaction, and determination of the level of sperm proteins associated with bull fertility potential. Total (92 ± 2%) and progressive (79 ± 4%) sperm motility; sperm concentration (1647 ± 429 × 10(6) sperm/mL); proportions of morphologically normal sperm (83 ± 6%) and DNA integrity (96 ± 2), and acrosome-intact sperm (64 ± 4%) exceeded minimum acceptable values. Frozen sperm had good total (58.7 ± 6.7%) and progressive (43.9 ± 9.2%) motility immediately after thawing. However, AI of 16 heifers resulted in no pregnancies and blastocyst production rate (following IVF using sperm from this infertile bull) was nearly identical to that produced using dead sperm (a control of parthenogenesis; 2 ± 2 and 2 ± 3%; respectively P < 0.05). Treatment with a calcium ionophore (A23187) failed to induce the acrosome reaction in sperm from the infertile bull (P < 0.05). Evaluation of several proteins associated with the fertility potential of bulls revealed that the level of Binder Sperm Protein-1 (BSP1), known to be associated with the capacitation process, was much greater on sperm from the infertile bull compared to that of his sire. In conclusion, we inferred that the idiopathic infertility in this bull was caused by a failure to complete the capacitation process. MenosThe objective of this case report was to identify the cause of apparent idiopathic infertility in a Red Angus (beef) bull. Semen was collected by electroejaculation and submitted to a series of assays, including evaluation of sperm motility by computer-assisted sperm analysis (CASA), sperm morphology and DNA integrity, semen cryopreservation, AI, IVF, induction of the acrosome reaction, and determination of the level of sperm proteins associated with bull fertility potential. Total (92 ± 2%) and progressive (79 ± 4%) sperm motility; sperm concentration (1647 ± 429 × 10(6) sperm/mL); proportions of morphologically normal sperm (83 ± 6%) and DNA integrity (96 ± 2), and acrosome-intact sperm (64 ± 4%) exceeded minimum acceptable values. Frozen sperm had good total (58.7 ± 6.7%) and progressive (43.9 ± 9.2%) motility immediately after thawing. However, AI of 16 heifers resulted in no pregnancies and blastocyst production rate (following IVF using sperm from this infertile bull) was nearly identical to that produced using dead sperm (a control of parthenogenesis; 2 ± 2 and 2 ± 3%; respectively P < 0.05). Treatment with a calcium ionophore (A23187) failed to induce the acrosome reaction in sperm from the infertile bull (P < 0.05). Evaluation of several proteins associated with the fertility potential of bulls revealed that the level of Binder Sperm Protein-1 (BSP1), known to be associated with the capacitation process, was much greater on sperm from the infertile bull compared to ... Mostrar Tudo |
Palavras-Chave: |
Absence of fertilization; Bovine infertility; Capacitation; Sperm morphology. |
Thesaurus Nal: |
sperm motility. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02419naa a2200253 a 4500 001 1908898 005 2024-02-05 008 2011 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.theriogenology.2011.04.020$2DOI 100 1 $aLESSARD, C. 245 $aInfertility in a beef bull due to a failure in the capacitation process.$h[electronic resource] 260 $c2011 520 $aThe objective of this case report was to identify the cause of apparent idiopathic infertility in a Red Angus (beef) bull. Semen was collected by electroejaculation and submitted to a series of assays, including evaluation of sperm motility by computer-assisted sperm analysis (CASA), sperm morphology and DNA integrity, semen cryopreservation, AI, IVF, induction of the acrosome reaction, and determination of the level of sperm proteins associated with bull fertility potential. Total (92 ± 2%) and progressive (79 ± 4%) sperm motility; sperm concentration (1647 ± 429 × 10(6) sperm/mL); proportions of morphologically normal sperm (83 ± 6%) and DNA integrity (96 ± 2), and acrosome-intact sperm (64 ± 4%) exceeded minimum acceptable values. Frozen sperm had good total (58.7 ± 6.7%) and progressive (43.9 ± 9.2%) motility immediately after thawing. However, AI of 16 heifers resulted in no pregnancies and blastocyst production rate (following IVF using sperm from this infertile bull) was nearly identical to that produced using dead sperm (a control of parthenogenesis; 2 ± 2 and 2 ± 3%; respectively P < 0.05). Treatment with a calcium ionophore (A23187) failed to induce the acrosome reaction in sperm from the infertile bull (P < 0.05). Evaluation of several proteins associated with the fertility potential of bulls revealed that the level of Binder Sperm Protein-1 (BSP1), known to be associated with the capacitation process, was much greater on sperm from the infertile bull compared to that of his sire. In conclusion, we inferred that the idiopathic infertility in this bull was caused by a failure to complete the capacitation process. 650 $asperm motility 653 $aAbsence of fertilization 653 $aBovine infertility 653 $aCapacitation 653 $aSperm morphology 700 1 $aSIQUEIRA, L. G. B. 700 1 $aD'AMOURS, O. 700 1 $aSULLIVAN, R. 700 1 $aLECLERC, P. 700 1 $aPALMER, C. 773 $tTheriogenology$gv. 76, n. 5, p. 891-899, 2011.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
28/08/2014 |
Data da última atualização: |
28/08/2014 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
ARANTES, L. de O.; POSSE, S. C. P.; RIVA-SOUZA, E. M.; ARANTES, S. D.; POSSE, R. P.; SANTANA, E. N. de; PEREIRA, H. S. |
Afiliação: |
LUCIO DE OLIVEIRA ARANTES, INCAPER; SHEILA CRISTINA PRUCOLI POSSE, INCAPER; ELAINE MANELLI RIVA-SOUZA, INCAPER; SARA DOUSSEAU ARANTES, INCAPER; ROBSON PRUCOLI POSSE, INSTITUTO FEDEAL DE EDUCAÇÃO, Colatina-ES; ENILTON NASCIMENTO DE SANTANA, INCAPER; HELTON SANTOS PEREIRA, CNPAF. |
Título: |
Avaliação de linhagens de feijoeiro comum no tabuleiro costeiro do Espírito Santo. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 11., 2014, Londrina. Tecnologias para a sustentabilidade da cultura do feijão: anais. Londrina: IAPAR, 2014. |
ISBN: |
978-85-88184-48-0 |
Idioma: |
Português |
Notas: |
CONAFE |
Conteúdo: |
Este trabalho tem o objetivo de divulgar os resultados dos Testes de Adaptação Local, sob a responsabilidade do Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural (INCAPER), conduzidos na região dos tabuleiros costeiros do Espírito Santo no período de 2010 a 2012. |
Palavras-Chave: |
Espirito Santo, ES. |
Thesagro: |
Feijão; Melhoramento genético vegetal; Phaseolus vulgaris. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/107440/1/441-1.pdf
|
Marc: |
LEADER 01149nam a2200253 a 4500 001 1993634 005 2014-08-28 008 2014 bl uuuu u00u1 u #d 020 $a978-85-88184-48-0 100 1 $aARANTES, L. de O. 245 $aAvaliação de linhagens de feijoeiro comum no tabuleiro costeiro do Espírito Santo.$h[electronic resource] 260 $aIn: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 11., 2014, Londrina. Tecnologias para a sustentabilidade da cultura do feijão: anais. Londrina: IAPAR$c2014 500 $aCONAFE 520 $aEste trabalho tem o objetivo de divulgar os resultados dos Testes de Adaptação Local, sob a responsabilidade do Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural (INCAPER), conduzidos na região dos tabuleiros costeiros do Espírito Santo no período de 2010 a 2012. 650 $aFeijão 650 $aMelhoramento genético vegetal 650 $aPhaseolus vulgaris 653 $aEspirito Santo, ES 700 1 $aPOSSE, S. C. P. 700 1 $aRIVA-SOUZA, E. M. 700 1 $aARANTES, S. D. 700 1 $aPOSSE, R. P. 700 1 $aSANTANA, E. N. de 700 1 $aPEREIRA, H. S.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|