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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
03/07/2021 |
Data da última atualização: |
31/08/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
VIANA, J. H. M.; PEREIRA, N. E. S.; FARIA, O. A. C.; DIAS, L. R. O.; OLIVEIRA, E. R.; FERNANDES, C. A. C.; SIQUEIRA, L. G. B. |
Afiliação: |
JOAO HENRIQUE MOREIRA VIANA, Cenargen; NATHALIA ELLEN SOUSA PEREIRA, UNB; OTAVIO AUGUSTO COSTA FARIA, UNB; LUZIA RENATA OLIVEIRA DIAS, UNB; EDUARDO RAMOS OLIVEIRA, UNIFENAS; CARLOS ANTONIO CARVALHO FERNANDES, UNIFENAS; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL. |
Título: |
Active immunization against GnRH as an alternative therapeutic approach for the management of Bos indicus oocyte donors diagnosed with chronic cystic ovarian disease. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Theriogenology, v. 172, p. 133-141, 2021. |
DOI: |
https://doi.org/10.1016/j.theriogenology.2021.06.014 |
Idioma: |
Inglês |
Conteúdo: |
The aim of this study was to evaluate the effect of active immunization against GnRH in Nelore (Bos indicus) cows repeatedly used as oocyte donors that developed chronic cystic ovarian disease (COD). In the first experiment, ovarian and uterine features were first compared between COD cows (n ¼ 15) and healthy cows (n ¼ 22, cycling control group) from the same breed and herd. Cows with COD had a greater number of large (P < 0.0001) and medium follicles (P < 0.01) but lesser small follicles (P < 0.05) than cycling controls. Mucometra was diagnosed in 73.3% of COD cows, but in none of the controls. No difference in average thickness of the endometrium was detected between groups; however, endometrial thickness and mucometra score were negatively correlated (R ¼ 0.73, P ¼ 0.0029) in COD cows. In the second experiment, COD cows were randomly allocated into two experimental groups, which received two 1.0 mL SC injections of either an anti-GnRH vaccine (COD immunized group, n ¼ 8) or saline (COD control group, n ¼ 7), given 28 days apart. Cows were examined weekly by transrectal ultrasonography during nine consecutive weeks after the first injection to evaluate the number and distribution of follicles among size classes, endometrial thickness, and presence of clinical mucometra. Vaccination against GnRH resulted in a progressive suppression of follicle growth and a reduction in the average size of the largest follicle, as well as in the number of large follicles (P < 0.01) in COD immunized cows compared with COD controls from week 7 onwards. Conversely, the number of small follicles in the COD immunized group increased after week 5 and was greater (P ¼ 0.0023) than controls on week 9. Endometrial thickness and mucometra score were not affected (P > 0.05) by immunization against GnRH. In the third experiment, the COD immunized cows with effective suppression of follicle growth four weeks after the 2nd injection (n ¼ 6) were submitted to three consecutive ovum pick-up (OPU) sessions (weeks 10, 11, and 12) for in vitro embryo production (IVEP). Cumulus-oocyte complexes (COC) collected from slaughterhouse ovaries were used as controls for IVEP. COD cows with produced 25.0 ± 3.8 COC per OPU session with no apparent detrimental effect of anti-GnRH vaccine on oocyte developmental potential in vitro, i.e., we observed similar cleavage rate (P ¼ 0.5914) and greater blastocyst rate (P ¼ 0.0177) in immunized cows compared with COC from slaughterhouse controls. Finally, in the fourth experiment wave emergence and follicular dynamics after follicle ablation were compared between COD immunized cows with effective suppression of follicle growth and a subset (n ¼ 6) of the cycling, control group. No follicles grew beyond 4 mm diameter after follicle ablation in the COD immunized group, whereas a normal follicular wave emergence occurred in cycling controls. Antral follicle count was similar between cycling controls and COD immunized groups at 24 h and 96 h post-follicle ablation (P > 0.05), but greater in cycling controls at 48 h and 72 h post-follicle ablation (P < 0.05). In summary, our results suggest that active immunization against GnRH is effective to induce the regression of follicular cysts as well as increase the number of small follicles growing on the ovaries, in oocyte donors diagnosed with chronic COD, with no apparent negative effect on oocyte developmental potential in vitro. MenosThe aim of this study was to evaluate the effect of active immunization against GnRH in Nelore (Bos indicus) cows repeatedly used as oocyte donors that developed chronic cystic ovarian disease (COD). In the first experiment, ovarian and uterine features were first compared between COD cows (n ¼ 15) and healthy cows (n ¼ 22, cycling control group) from the same breed and herd. Cows with COD had a greater number of large (P < 0.0001) and medium follicles (P < 0.01) but lesser small follicles (P < 0.05) than cycling controls. Mucometra was diagnosed in 73.3% of COD cows, but in none of the controls. No difference in average thickness of the endometrium was detected between groups; however, endometrial thickness and mucometra score were negatively correlated (R ¼ 0.73, P ¼ 0.0029) in COD cows. In the second experiment, COD cows were randomly allocated into two experimental groups, which received two 1.0 mL SC injections of either an anti-GnRH vaccine (COD immunized group, n ¼ 8) or saline (COD control group, n ¼ 7), given 28 days apart. Cows were examined weekly by transrectal ultrasonography during nine consecutive weeks after the first injection to evaluate the number and distribution of follicles among size classes, endometrial thickness, and presence of clinical mucometra. Vaccination against GnRH resulted in a progressive suppression of follicle growth and a reduction in the average size of the largest follicle, as well as in the number of large follicles (P < 0.01) in COD ... Mostrar Tudo |
Palavras-Chave: |
Follicle growth suppression; GnRH vaccine; In vitro embryo production; Nelore breed. |
Thesagro: |
Bovino; Doença Animal; Gado Nelore. |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 04414naa a2200289 a 4500 001 2133972 005 2021-08-31 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.theriogenology.2021.06.014$2DOI 100 1 $aVIANA, J. H. M. 245 $aActive immunization against GnRH as an alternative therapeutic approach for the management of Bos indicus oocyte donors diagnosed with chronic cystic ovarian disease.$h[electronic resource] 260 $c2021 520 $aThe aim of this study was to evaluate the effect of active immunization against GnRH in Nelore (Bos indicus) cows repeatedly used as oocyte donors that developed chronic cystic ovarian disease (COD). In the first experiment, ovarian and uterine features were first compared between COD cows (n ¼ 15) and healthy cows (n ¼ 22, cycling control group) from the same breed and herd. Cows with COD had a greater number of large (P < 0.0001) and medium follicles (P < 0.01) but lesser small follicles (P < 0.05) than cycling controls. Mucometra was diagnosed in 73.3% of COD cows, but in none of the controls. No difference in average thickness of the endometrium was detected between groups; however, endometrial thickness and mucometra score were negatively correlated (R ¼ 0.73, P ¼ 0.0029) in COD cows. In the second experiment, COD cows were randomly allocated into two experimental groups, which received two 1.0 mL SC injections of either an anti-GnRH vaccine (COD immunized group, n ¼ 8) or saline (COD control group, n ¼ 7), given 28 days apart. Cows were examined weekly by transrectal ultrasonography during nine consecutive weeks after the first injection to evaluate the number and distribution of follicles among size classes, endometrial thickness, and presence of clinical mucometra. Vaccination against GnRH resulted in a progressive suppression of follicle growth and a reduction in the average size of the largest follicle, as well as in the number of large follicles (P < 0.01) in COD immunized cows compared with COD controls from week 7 onwards. Conversely, the number of small follicles in the COD immunized group increased after week 5 and was greater (P ¼ 0.0023) than controls on week 9. Endometrial thickness and mucometra score were not affected (P > 0.05) by immunization against GnRH. In the third experiment, the COD immunized cows with effective suppression of follicle growth four weeks after the 2nd injection (n ¼ 6) were submitted to three consecutive ovum pick-up (OPU) sessions (weeks 10, 11, and 12) for in vitro embryo production (IVEP). Cumulus-oocyte complexes (COC) collected from slaughterhouse ovaries were used as controls for IVEP. COD cows with produced 25.0 ± 3.8 COC per OPU session with no apparent detrimental effect of anti-GnRH vaccine on oocyte developmental potential in vitro, i.e., we observed similar cleavage rate (P ¼ 0.5914) and greater blastocyst rate (P ¼ 0.0177) in immunized cows compared with COC from slaughterhouse controls. Finally, in the fourth experiment wave emergence and follicular dynamics after follicle ablation were compared between COD immunized cows with effective suppression of follicle growth and a subset (n ¼ 6) of the cycling, control group. No follicles grew beyond 4 mm diameter after follicle ablation in the COD immunized group, whereas a normal follicular wave emergence occurred in cycling controls. Antral follicle count was similar between cycling controls and COD immunized groups at 24 h and 96 h post-follicle ablation (P > 0.05), but greater in cycling controls at 48 h and 72 h post-follicle ablation (P < 0.05). In summary, our results suggest that active immunization against GnRH is effective to induce the regression of follicular cysts as well as increase the number of small follicles growing on the ovaries, in oocyte donors diagnosed with chronic COD, with no apparent negative effect on oocyte developmental potential in vitro. 650 $aBovino 650 $aDoença Animal 650 $aGado Nelore 653 $aFollicle growth suppression 653 $aGnRH vaccine 653 $aIn vitro embryo production 653 $aNelore breed 700 1 $aPEREIRA, N. E. S. 700 1 $aFARIA, O. A. C. 700 1 $aDIAS, L. R. O. 700 1 $aOLIVEIRA, E. R. 700 1 $aFERNANDES, C. A. C. 700 1 $aSIQUEIRA, L. G. B. 773 $tTheriogenology$gv. 172, p. 133-141, 2021.
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Embrapa Gado de Leite (CNPGL) |
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Registros recuperados : 164 | |
61. | | CHEN, Z.; HAGEN, D. E.; WANG, J.; ELSIK, C. G.; JI, T.; SIQUEIRA, L. G. B.; HANSEN, P. J.; RIVERA, R. M. Global assessment of imprinted gene expression in the bovine conceptus by next generation sequencing. Epigenetics, v. 11, n. 7, p. 501-516, 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Gado de Leite. |
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62. | | FONSECA, J. F. da; VERGANI, G. B.; CÔRTES, L. R.; RODRIGUES, J. N. D.; SIQUEIRA, L. G. B.; SOUZA-FABJAN, J. M.; OLIVEIRA, M. E. F. Induction of accessory corpus luteum in goats and sheep: from physiological basis to reproductive effects. Revista Brasileira de Reprodução Animal, v. 45, n. 4, p. 309-317, out./dez. 2021. Edição dos anais do XXIV Congresso Brasileiro de Reprodução Animal e VIII International Symposium on Animal Biology of Reproduction, Belo Horizonte, 2021.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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63. | | FONSECA, J. F. da; VERGANI, G. B.; CÔRTES, L. R.; RODRIGUES, J. N. D.; SIQUEIRA, L. G. B.; SOUZA-FABJAN, J. M.; OLIVEIRA, M. E. F. Induction of accessory corpus luteum in goats and sheep: From physiological basis to reproductive effects. Revista Brasileira de Reprodução Animal, v. 45, n. 4, p. 309-317, out./dez. 2021. Anais do XXIV Congresso Brasileiro de Reprodução Animal (CBRA-2021) e VIII International Symposium on Animal Biology of Reproduction - Joint Meeting, Belo Horizonte, MG, 19 a 22 de outubro de 2021.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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64. | | WOHLRES-VIANA, S.; ARASHIRO, E. K. N; MACHADO, M. A.; CAMARGO, L. S. de A.; SIQUEIRA, L. G. B.; PALHÃO, M. P.; VIANA, J. H. M. Intrafollicular oestradiol production, expression of the LH receptor (LHR) gene and its isoforms, and early follicular deviation in Bos indicus. Reproduction Fertility and Development, v. 29, n. 10, p. 1958-1970, 2017. Publicado online em 19 nov. 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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65. | | SIQUEIRA, L. G. B.; TORRES, C. A. A.; AMORIM, L. S.; SOUZA, E. D.; CAMARGO, L. S. de A.; FERNANDES, C. A. C.; VIANA, J. H. M. Interrelationships among morphology, echotexture, and function of the bovine corpus luteum during the estrous cycle. Animal Reproduction Science, v. 115, n. 1/4, p. 18-28, 2009.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Gado de Leite. |
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67. | | ARASHIRO, E. K. N.; VIEIRA, D. S.; PFEIFER, L. F. M.; SIQUEIRA, L. G. B.; CAMARGO, L. S. de A.; FERNANDES, C. A. C.; VIANA, J. H. M. Induction of ovulation with estradiol benzoate affects the progression of vascularization in preoculatory follicles. n: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 2013, Hannover. Proceedings... Hannover: [s.n.], 2013.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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69. | | SCHWARZ, D. G. G.; OLIVEIRA, T. S. A. M. de; MALAQUIAS, J. V.; BRAVO, R. P. B.; SIQUEIRA, L. G. B.; MARTINS, C. F. Influência da idade e condição corporal no comportamento em pastejo de ovinos Santa Inês no Bioma Cerrado. In: ENCONTRO DE JOVENS TALENTOS DA EMBRAPA CERRADOS, 4., 2009, Planaltina, DF. Resumos apresentados... Planaltina, DF: Embrapa Cerrados, 2009. p. 169-170 (Embrapa Cerrados. Documentos, 243).Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Cerrados. |
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71. | | SIQUEIRA, L. G. B.; VIANA, J. H. M.; DINIZ, E. S.; CAMARGO, L. S. A.; OLIVEIRA, A. P.; FERNANDES, C. A. C.; TORRES, C. A. A. Luteal tissue area in embryo recipients synchronized and with spontaneous or estradiol benzoate induced ovulation. Acta Scientiae Veterinariae, v. 34, s494, 2006. Supl. 1. Edição dos resumos da 20ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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72. | | CAMARGO, L. S. de A.; QUINTAO, C. C. R.; DENICOL, A. C.; IGUMA, L. T.; CARVALHO, B. C. de; SIQUEIRA, L. G. B.; VIANA, J. H. M. Efeito da tricostatina A na produção de embriões bovinos por transferência nuclear com células somáticas e subsequente gestações. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, 25., 2011, Cumbuco. Resumos... p. 436Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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73. | | AMORIM, L. S.; TORRES, C. A. A.; AMORIM, E. A. M.; GUIMARÃES, J. D.; FONSECA, J. F. da; SIQUEIRA, L. G. B.; SEIDEL, G. E. Effect of dietary urea on embryonic viability and development of Toggenburg goats. Reproduction, Fertility and Development, v.21, n. 1, p. 151, 2009. Edição dos proceedings da Annual Conference or the International Embryo Transfer Society, San Diego, California, 2009.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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74. | | VIEIRA, A. C.; QUINTAO, C. C. R.; SIQUEIRA, L. G. B.; OLIVEIRA, C. S.; SILVA, T. A.; VIEIRA, C. D.; CAMARGO, L. S. de A. Effect of epigenetic modulators on development of bovine embryos derived from heatshocked oocytes. Animal Reproduction, v. 15, n. 3, p. 483, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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75. | | SIQUEIRA, L. G. B.; SEQUETO, R.; ANUNCIAÇÃO, R.; CAMARGO, L. S. de A.; FERNANDES, C.; FONSECA, J. F. da; PFEIFER, L. F. M.; VIANA, J. H. M. Hormonal profile during resynchronisation using oestradiol benzoate and progesterone-based protocols associated or not with flunixin meglumine in cattle. Reproduction, Fertility, and Development, v. 35, n. 1/2, p. 218-219, 2023. Edição dos Proceedings of the 49th Annual Conference of the International Embryo Technology, Lima, Peru, 2023.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Rondônia. |
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76. | | MAIA, A. L. R. S.; BRANDÃO, F. Z.; SOUZA-FABJAN, J. M. G.; ARAÚJO, M. C. C.; SIQUEIRA, L. G. B.; FACO, O.; FONSECA, J. F. da. Hydrosalpinx in dairy goats: occurrence, ultrasound diagnosis, macro- and microscopic characterization. Small Ruminant Research, v. 160, p. 5-11, 2018.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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77. | | CAMARGO, L. S. de A.; GONÇALVES, F. S.; QUINTAO, C. C. R.; IGUMA, L. T.; SOUZA, E. D.; SIQUEIRA, L. G. B.; VIANA, J. H. M. Genetic modification of bovine embryos by lentiviral vectors. Animal Reproduction, v.10, n.3, p. 359, Jul./Sept. 2013.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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78. | | GRAZIA, J. G. V.; LACERDA, L. G.; SIQUEIRA, L. G. B.; PELLEGRINO, C. A. G.; GRAPIUNA, L. S.; SOLLECITO, N. V.; VIANA, J. H. M. Effects of Gas Tension During Culture upon Development of Bovine Embryos from Beef (Nellore) and Dairy (Girolando) Breeds. In: International Embryo Technology Society and the Society for Reproductive Biology, v. 30 n. 1. Resumo em Anais. Bangkok, Tailandia. IETS, 2018. 207.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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79. | | CAMARGO, L. S. de A.; SIQUEIRA, L. G. B.; SARAIVA, N. Z.; MAIA, G.; NOGUEIRA, B.; QUINTAO, C. C. R.; OLIVEIRA, C. S. Effect of physiological heat stress on HSP70 and CASPASE 3 levels in bovine oocytes. In: ANNUAL CONFERENCE, 52., 2019, San Jose. Beyond possible: remarkable transformation of reproductive biology: abstracts. San Jose: Society for the Study of Reproduction, 2019.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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80. | | MOURA, R. M. de; MARTINS, L. P.; SIQUEIRA, L. G. B.; FERNANDES, C. A. C.; MARTINS, C. F.; FIGUEIREDO, R. A.; VIANA, J. H. M. Effect of the route of administration of recombinant human FSH on ovarian superestimulatory response in calves. Animal Reproduction, v. 19, n. 2, 2022. e22084. Proceedings of the 35rd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, August 2022. Abstracts.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 164 | |
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